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| Id: | 19661
| | Author: | Roche, Paul W; Failbus, Sarah S; Britton, Warwick J; Cole, Robert. | | Title: | Rapid method for diagnosis of leprosy by measurements of antibodies to the M. leprae 35-kDa protein: comparison with PGL-1 antibodies detected by ELISA and "Dipstick" methods.
| | Source: | Int. J. Lepr;67(3):279-286, Sept., 1999. ilus, graf.
| | Abstract: | A new rapid immuno-chromatographic test card for the detection of antibodies to the Mycobacterium leprae 35-kD protein is described. The new assay is compared in the same group of subjects with a direct enzyme ELISA method for 35-kD antibodies and with assays for anti-phenolic glycolipid-I (PGL-I) antibodies using a standard ELISA as well as the recently described [quot ]dipstick[quot ] method. Good concordance was found between the rapid methods and the corresponding ELISA methods. The detection of untreated paucibacillary leprosy by the 35-kD test card was 59% compared with 27% for the PGL-I dipstick; however, the specificity for the 35-kD test card was 90% compared with 100% for the PGL-I dipstick in an endemic population. The potential application of these new, rapid serologic methods for the diagnosis of leprosy under field conditions is discussed. (AU)^ien.
| | Descriptors: | Mycobacterium leprae/genet
Mycobacterium leprae/fisiol
Anexina A3/imunol
ELISA/métodos
| | Limits: | Humanos
| | Electronic Medium: | http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a07.pdf / en
| | Location: | BR191.1 |
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