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| Id: | 13942
| | Author: | Maeda, Yumi; Gidoh, Masaichi; Ishii, Norihisa; Mukai, Chifumi; Makino, Masahiko
| | Title: | Assessment of cell mediated immunogenicity of Mycobacterium leprae-derived antigens
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| | Source: | s.l; s.n; 2003. 9 p. tab, graf.
| | Abstract: | The antigenicity of Mycobacterium leprae (M. leprae)-derived cell membrane fraction was examined using human dendritic cells (DCs). Immature DCs internalized and processed the cell membrane components, and expressed M. leprae-derived antigens (Ags) on their surface. The expression of MHC class II, CD86, and CD83 Ags on DCs and CD40 ligand (L)-associated IL-12 p70 production from DCs were up-regulated by the membrane Ags. Moreover these stimulated DCs induced significantly higher level of interferon-gamma (IFN-gamma) production by autologous CD4(+) and CD8(+) T cells than those pulsed with equivalent doses of live M. leprae or its cytosol fraction. Both subsets of T cells from tuberculoid leprosy patients also produced several fold more IFN-gamma than those from normal individuals. Furthermore, the intracellular perforin production in CD8(+) T cells was up-regulated in an Ag-dose dependent manner. These results suggest that M. leprae membrane Ags might be useful as the vaccinating agents against leprosy. (AU).
| | Descriptors: | ANTIGENOS DE BACTERIAS/imunol
ANTIGENOS DE SUPERFÍCIE/imunol
LINFOCITOS T CD4-POSITIVOS/imunol
LIGANTE A CD40/fisiol
LINFOCITOS T CD8-POSITIVOS/imunol
CELULAS DENDRITICAS/imunol
INTERFERON TIPO II/bios
INTERLEUCINA-12/bios
TRANSFORMACAO LINFOCITICA
GLICOPROTEINAS DE MEMBRANA/bios
MYCOBACTERIUM LEPRAE/imunol
| | Limits: | HUMANO
SUPPORT, NON-U.S. GOV'T
SUPPORT, U.S. GOV'T, P.H.S.
| | Electronic Medium: | http://www.ilsl.br
| | Location: | BR191.1; 09083/s |
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