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| Id: | 19533
| | Author: | Gormus, Bobby J; Baskin, Gary B; Xu, Keyu; Ratterree, Marion S; Martin, Louis N; Mack, Pamela A; Bohm Jr, Rudolf P; Meyers, Wayne M; Walsh, Gerald P. | | Title: | Antileprosy protective vaccination of rhesus monkeys with BCG or BCG plus heat-killed mycobacterium leprae: immunologic observations.
| | Source: | Int. J. Lepr;68(1):27-39, Mar., 2000. graf.
| | Abstract: | Groups of rhesus monkeys were vaccinated and boosted with Mycobacterium bovis bacillus Calmette Guerin (BCG) or BCG plus low-dose (LD) or high-dose (HD) heat-killed M. leprae (HKML), or were unvaccinated. Prior to and following vaccination-boosting and subsequent M. leprae (ML) challenge, these and unvaccinated, unchallenged control monkeys were observed longitudinally for approximately 3 years. Vaccination with BCG plus HKML initially stimulated significant in vitro blood mononuclear cell blastogenic responses to lepromin, which returned to baseline post-boosting and post-live-ML-challenge, minimally reappearing significantly 2 years post-ML-challenge. Vaccination with BCG failed to stimulated positive blastogenic responses to lepromin before ML-challenge but small, marginally positive, intermittent responses were seen post-ML-challenge. Compared to the unvaccinated ML-challenged group, significant increases in the numbers of blood CD4+ and CD8+ T-cell subsets and an increased CD4+:CD8+ ratio were observed in both BCG plus HKML-vaccinated, ML-challenged groups, but not in the BCG-only-vaccinated, ML-challenged group. CD4+CD29+ and CD4+CD45RA+ subset numbers increased significantly over time in only the BCG plus LD HKML-vaccinated, ML-challenged group. Compared to unvaccinated, ML-challenged groups, vaccination with BCG or BCG plus HKML followed by ML-challenge produced lower IgM:IgG antiphenolic glycolipid-I (PGL-I) serum antibody ratios and protected rhesus monkeys from clinical leprosy, consistent with prior observations that low IgM:IgG anti-PGL-I responses correlated with resistance to and protection from leprosy. (AU)^ien.
| | Descriptors: | Mycobacterium leprae/imunol
Macaca mulatta/genet
Macaca mulatta/imunol
Vacina BCG/imunol
Vacina BCG/uso terap
| | Limits: | Animais
| | Electronic Medium: | http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2000/pdf/v68n1/v68n1a05.pdf / en
| | Location: | BR191.1 |
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