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| Id: | 13722
| | Author: | Maeda, Yumi; Makino, Masahiko; Crick, Dean C; Mahapatra, Sebabrata; Srisungnam, Sopa; Takii, Takemasa; Kashiwabara, Yoshiko; Brennan, Patrick J
| | Title: | Novel 33-kilodalton lipoprotein from Mycobacterium leprae
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| | Source: | s.l; s.n; 2002. 6 p. tab, graf.
| | Abstract: | A novel Mycobacterium leprae lipoprotein LpK (accession no. ML0603) was identified from the genomic database. The 1,116-bp open reading frame encodes a 371-amino-acid precursor protein with an N-terminal signal sequence and a consensus motif for lipid conjugation. Expression of the protein, LpK, in Escherichia coli revealed a 33-kDa protein, and metabolic labeling experiments and globomycin treatment proved that the protein was lipidated. Fractionation of M. leprae demonstrated that this lipoprotein was a membrane protein of M. leprae. The purified lipoprotein was found to induce production of interleukin-12 in human peripheral blood monocytes. The studies imply that M. leprae LpK is involved in protective immunity against leprosy and may be a candidate for vaccine design. (AU).
| | Descriptors: | SEQUENCIA DE AMINOACIDOS
ANTIGENOS DE BACTERIAS/GE/*IM/IP
PROTEINAS DE BACTERIAS/GE/*IM/IP
CLONAGEM MOLECULAR
ESCHERICHIA COLI
EXPRESSAO GENICA
GENES BACTERIANOS
INTERLEUCINA-12/*BI
LIPOPROTEINAS/GE/*IM/IP
PROTEINAS DE MEMBRANA/GE/*IM/IP
DADOS DE SEQUENCIA MOLECULAR
PESO MOLECULAR
MONOCITOS/IM
MYCOBACTERIUM LEPRAE/GE/*IM/IP
PRECURSORES DE PROTEINAS/GE/*IM/IP
ANALISE DE SEQUENCIA DE DNA
| | Limits: | HUMANO
SUPPORT, NON-U.S. GOV'T
SUPPORT, U.S. GOV'T, P.H.S.
| | Location: | BR191.1; 09059/s |
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