Database : HANSEN Search on : SEQUENCIAS REPETITIVAS DO ACIDO NUCLEICO [Subject descriptor] References found : 2 [refine] Displaying: 1 .. 2   in format [Detailed]

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Id: 13719 Author: Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P Title: Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..- Source: s.l; s.n; 2003. 5 p. tab. Abstract: To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue PROTEINAS DE BACTERIAS/genet HANSENIASE/diag HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/isol REACAO EM CADEIA DA POLIMERASE/métodos PELE/microbiol - SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO  PRIMERS DO DNA Limits: ESTUDO COMPARATIVO HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09117/s

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Id: 13639 Author: Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P Title: Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..- Source: s.l; s.n; 2003. 5 p. ilus, tab. Abstract: To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue PROTEINAS DE BACTERIAS/genet PRIMERS DO DNA HANSENIASE/diag HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/isol REACAO EM CADEIA DA POLIMERASE/métodos SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO PELE/microbiol Limits: ESTUDO COMPARATIVO HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09054/s

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