Database : HANSEN Search on : IMUNOGLOBULINA G/IMUNOL [Subject descriptor] References found : 4 [refine] Displaying: 1 .. 4   in format [Detailed]

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Id: 19589 Author: Desforges, Sylvie; Bobin, Pierre; Brethes, Bernard; Huerre, Michel; Moreau, Jean-Paul; Bach, Marie-Ann. Title: Specific anti M. Leprae PHL-1 antibodies and mitsuda reactions in the management of household contacts in New Caledonia. Source: Int. J. Lep;57(4):794-800, dec. 1989. ^btab, ^bgraf. Abstract: Household contacts of leprosy patients have been tested for anti-phenolic glycolipid-I IgM antibodies (anti-PGL-I IgM) by an ELISA using the natural disaccharide (NDO) and natural trisaccharide (NTO) synthetic antigens. A group of healthy subjects without known exposure to Mycobacterium leprae served as controls. The percentages of positivity observed in multibacillary patients, paucibacillary patients, and household contacts were significantly higher than those of the negative controls. The absorbance values using NDO and NTO correlated well (range 0.59-0.91) when analysis of each subject group was performed. As reported here, NDO and NTO antigens seem to be equal in detecting leprosy cases; 100% of multibacillary and 21.43% of paucibacillary cases were detected as seropositive. For the screening of household contacts, NDO appears to be more sensitive and NTO more specific. There were more seropositive cases in the young age groups of household contacts, suggesting a higher rate of transmission of M. leprae infection in those age groups. Lepromin and anti-PGL-I IgG tests were also performed in contacts who were followed. The 2 paucibacillary subjects (1 borderline tuberculoid, 1 indeterminate) were Mitsuda negative. At diagnosis, their anti-PGL-I IgM levels were much higher than those of previous results; their anti-PGL-I IgG levels showed an increase in one and a decrease in the other. However, for the entire group anti-PGL-I IgM and anti-PGL-I IgG levels were positively correlated. The data reported here suggest that an increase in specific anti-M. leprae IgM levels in Mitsuda-negative household contacts could reveal the development of overt disease. Descriptors: Imunoglobulina G/imunol Imunoglobulina M/imunol Glicolipídeos/imunol Mycobacterium leprae/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n4/v57n4a07.pdf / en Location: Br191.1

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Id: 19586 Author: Hasan, Rumina; Dockrell, Hazel M; Chiang, Thomas; Hussain, Rabia. Title: Quantitative antibody ELISA for leprosy. Source: Int. J. Lep;57(4):766-776, dec. 1989. ^btab, ^bgraf. Abstract: Quantitative enzyme-linked immunosorbent assays (ELISAs) were established to measure IgM and IgG antibody levels to soluble Mycobacterium leprae sonicate (CD60) and to the synthetic disaccharide antigen based on the phenolic glycolipid-I antigen of M. leprae coupled to bovine serum albumin in 46 leprosy patients. Separate reference pools for IgM and IgG antibody were established. The reciprocal of the antibody titer was expressed as the number of arbitrary units in the reference pools which was subsequently used as the calibrator for assessment of units in individual test sera. The dose-response relationship for both IgM and IgG was highly specific and reproducible for both isotypes, as indicated by the intra- and inter-assay coefficients of variation. The distribution of antibody levels are in general agreement with the results from previous studies against different M. leprae antigens. The lepromatous group showed 10- to 100-fold higher IgM antibodies to both the soluble sonicate antigen and the disaccharide as compared to the control group. Very low to undetectable levels of IgM antibodies were observed in the tuberculoid group of leprosy patients. IgG antibodies, on the other hand, were not only present but showed considerable overlap with the lepromatous patient group. Optimized ELISAs, such as the one described in this study, would allow one to address issues such as antibody changes with treatment, antigen clearance, and correlation with other immune parameters associated with disease pathogenesis and protection. Descriptors: Anticorpos Antibacterianos/imunol Imunoglobulina G/imunol Imunoglobulina M/imunol Mycobacterium leprae/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n4/v57n4a04.pdf / en Location: Br191.1

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Id: 18234 Author: Das, Pranab K; Rambukkan, Anura; Baas, J. G; Groothuuis, Dick G; Halperin, Moshe Title: Enzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis ..- Source: s.l; s.n; Feb. 1990. 4 p. ilus, tab. Abstract: Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and 28 tuberculoid leprosy patient reacted in a significant amnner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy pateints, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguihing two polar types of leprosy and for diagnosing leprosy in general (AU). Descriptors: HANSENIASE VIRCHOWIANA/imunol HANSENIASE TUBERCULOIDE/imunol MYCOBACTERIUM TUBERCULOSIS/imunol ELISA/util IMUNOGLOBULINA G/imunol IMUNOGLOBULINA G/ultraest - HANSENIASE/imunol  MICOBACTERIOSE/imunol Limits: HUMANO Location: BR191.1; 09295/s

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Id: 13500 Author: Mehra, Vijay; Mason, Llewellyn H; Fields, James P; Bloom, Barry R Title: Lepromin-induced suppressor cells in patients with leprosy ..- Source: s.l; s.n; oct. 1979. 5 p. tab, graf. Abstract: The possibility of an active mechanism of immunologic suppression in leprosy was explored by assessing the in vitro lymphocyte responses of 61 leprosy patients and 30 normal individuals to the mitogen Con A in the presence or absence of Dharmendra lepromin. Lepromin-induced suppression of Con A stimulation was found in 32 of 35 lepromatous patients and 15 of 15 borderline patients, but only 2 of 15 tuberculoid patients and 2 of 30 normal controls. Cell fractionation studies indicated at least two cell populations involved in the in vitro lepromin-induced suppressor activity, adherent cells and T gamma-cells.(AU). Descriptors: LINFOCITOS/clas TRANSFORMACAO LINFOCITICA HANSENIASE/imunol FORMACAO DE ROSETA LINFOCITOS T SUPRESSORES-EFETORES/imunol IMUNOGLOBULINA G/imunol ANTIGENO DE MITSUDA/imunol RECEPTORES FC/imunol CONCANAVALINA A/farmacol ADERÊNCIA CELULAR Limits: HUMANO SUPPORT, U.S. GOV'T, P.H.S. Electronic Medium: http://www.ilsl.br Location: BR191.1; 01400/s

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