Database : HANSEN Search on : RADIACAO [Subject descriptor] References found : 5 [refine] Displaying: 1 .. 5   in format [Detailed]

page 1 de 1

1 / 5

HANSEN

select to print Texto Completo-en

Id: 19529 Author: Adams, Linda B; Soileau, Nashone A; Battista, John R; Krahenbuhl, James L. Title: Inhibition of metabolism and growth of mycobacterium leprae by Gamma irradiation. Source: Int. J. Lepr;68(1):1-10, Mar., 2000. ilus, graf. Abstract: Mycobacterium leprae is uncultivable on artificial medium, but viability can be maintained without multiplication for a limited time in vitro. In this study, we evaluated gamma-irradiation (gamma-irr) as a means to kill this slowly growing organism. Freshly harvested, viable, athymic, nu/nu mouse-derived M. leprae were exposed to varying doses of gamma-irr from a 60Co source. Two indicators of bacterial viability were determined: metabolism, measured by oxidation of 14C-palmitic acid to 14CO2 in the BACTEC 460 system, and multiplication, measured by titration in the mouse foot pad. gamma-Irr of both M. leprae and M. lufu, a cultivable control mycobacterium, resulted in a dose-dependent inhibition of viability. gamma-Irr of up to 10(3) rad had little effect on the metabolic activity of either organism. For M. leprae, 10(4)-10(5) rad caused an intermediate inhibitory effect; whereas 10(6) rad yielded almost total inhibition. In the mouse foot pad assay, up to 10(4) rad had little effect on M. leprae growth; however, 10(5) rad resulted in at least a 2-log reduction in the number of bacilli recovered and no M. leprae growth was measurable after exposure to 10(6) rad. With M. lufu, 10(5) rad inhibited metabolic activity by 99% and caused > or = 2-log reduction in the number of colony forming units (CFU). No CFU of M. lufu were recovered after exposure to 10(6) rad. Scanning electron microscopy revealed the presence of some aberrant protrusions on the cell surface of lethally irradiated M. leprae; whereas boiling and autoclaving caused obvious morphological denaturation. These data suggest that gamma-irr is an effective way to kill M. leprae without causing extensive damage to the cell architecture. Killing M. leprae by gamma-irr may be preferable when comparing cellular responses to live versus dead bacilli in vitro and in vivo. (AU)^ien. Descriptors: Mycobacterium leprae/metab Mycobacterium leprae/fisiol Usos da Radiação Raios gama/uso terap Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2000/pdf/v68n1/v68n1a01.pdf / en Location: BR191.1

2 / 5

HANSEN

select to print

Id: 17967 Author: Rabe JH; Mamelak AJ; McElgunn PJ; Morison WL; Sauder DN Title: Photoaging: mechanisms and repair Photoaging: mechanisms and repair-v.55 Source: s.l; s.n; 2006. 19 p. ilus, tab, graf. Abstract: Aging is a complex, multifactorial process resulting in several functional and esthetic changes in the skin. These changes result from intrinsic as well as extrinsic processes, such as ultraviolet radiation. Recent advances in skin biology have increased our understanding of skin homeostasis and the aging process, as well as the mechanisms by which ultraviolet radiation contributes to photoaging and cutaneous disease. These advances in skin biology have led to the development of a diversity of treatments aimed at preventing aging and rejuvenating the skin. The focus of this review is the mechanism of photoaging and the pathophysiology underlying the treatments specifically designed for its prevention and treatment. LEARNING OBJECTIVES: At the conclusion of this learning activity, participants should be familiar with the mechanism of photoaging, the treatments for photoaging, and the data that supports the use of these treatments...(AU). Descriptors: PELE/anat PELE/citol PELE/les PELE/ef rad IMUNOSSUPRESSAO/instrum IMUNOSSUPRESSAO/métodos USOS DA RADIACAO Limits: ESTUDO COMPARATIVO HUMANO Location: BR191.1; 00322/cme

3 / 5

HANSEN

select to print

Id: 11572 Author: França, Emmanuel Rodrigues de Title: A UVB-suscetibilidade na hanseníase ?- Source: Rio de Janeiro; s.n; 1996. 142 p. ilus, tab. Thesis: Present for Universidade Federal do Rio de Janeiro. Faculdade de Medicina presented for the degree Doutor. Abstract: Fundamentos - A radiação ultravioleta é capaz de modificar as respostas imunológicas mediadas por células nos modelos animais e no homem. Está bem estabelecido que ela pode interferir no comportamento das infecções virais, fúngicas, helmínticas e bacterianas. As células de Langerhans representam a forma inicial e principal de contato do sistema imunológico com os antígenos ambientais, dentre eles os bacterianos. A radiação ultravioleta promove uma depleção quantitativa e funcional destas células, possibilitando uma resposta orgânica alterada à invasão de microrganismos. Existe possibilidade de que a infecção pelo M. leprae seja afetada por este fenômeno. Objetivos - Os objetivos deste trabalho foram: determinar através do uso do dinitroclorobenzano (DNCB) a freqüência de indivíduos UVB-suscetíveis (UVB-S) e UVB-resistentes (UVB-R) nos diferentes grupos clínicos de hansenianos e no grupo controle; tentar estabelecer se existe relação entre UVB-suscetibilidade e a resposta à reação de Mitsuda nos indivíduos estudados; detectar a incidência de lesões hansênicas nas formas clínicas I, TT e BT em áreas normalmente expostas e não expostas à radiação ultravioleta. Metodologia - Foram selecionados 186 voluntários divididos em 2 grupos. Um grupo controle com 64 indivíduos e um grupo de 122 hansenianos. Os hansenianos foram identificados de acordo com os critérios clínicos e histopatológicos da classificação de Ridley e Jopling e foram submetidos ao teste de Mitsuda. Em todos, foi determinada a dose eritematosa mínima (DEM) de UVB. Vinte e quatro horas após foi aplicada uma dose de 2000 ug de DNCB numa área média ou baixa do dorso irradiada com 4 DEM, sendo realizada a leitura deste procedimento 48 horas após. Depois de trinta dias foram aplicados em uma área não irradiada 50 ug de DNCB , sendo a leitura feita 48 horas após a aplicação. Em 3 indivíduos foi verificado o comportamento das células de Langerhans, após identificação pela proteína S-100, na pele normal e na pele contralateral irradiada com 4,5 Kj/m2 de UVB. Resultados - O grupo TT mostrou 67,25 por cento de indivíduos UVB-R e 32,25 por cento de indivíduos UVB-S. O grupo BT revelou que 75 por cento dos indivíduos eram UVB-R e 25 por cento UVB-S. Os grupos I e BB também apresentaram percentuais próximos ao grupo controle, ou seja, no grupo I havia 69,23 por cento de indivíduos UVB-R e 30,77 por cento de indivíduos UVB-S e no grupo BB 66,67 por cento eram UVB-R e 33,33 popr cento UVB-S. (AU). Descriptors: HANSENIASE RAIOS ULTRAVIOLETA SUSCETIBILIDADE A DOENÇA CÉLULAS DE LANGERHANS INFECÇAO RADIAÇAO Location: BR191.1; WC335.500, F844u

4 / 5

HANSEN

select to print

Id: 439 Author: Congdon, C. C; Payne, H; Gengozian, N. Title: Immune status at 12 months in thymectomized radiation chimeras. Source: In: Annual Leprosy Research Conference, 7.Annual Leprosy Research Conference, 7/Abstracts. California, National Institute of Health, 1972. p.14. Conference: Present in: Annual Leprosy Research Conference, 7, California, 29 Feb.-01 Mar. 1972. Descriptors: CAMUNDONGOS NUDE QUIMERA DE RADIAÇAO Limits: RELATO DE CASO CAMUNDONGOS Location: BR191.1; WC335.3, 78a

5 / 5

HANSEN

select to print

Id: 438 Author: Fieldsteel, A. Howard. Title: Mycobacterium leprae infection in neonatally thymectomized lewis rats: effect of radiation and antithymocytic screen. Source: In: Annual Leprosy Research Conference, 7.Annual Leprosy Research Conference, 7/Abstracts. California, National Institute of Health, 1972. p.13. Conference: Present in: Annual Leprosy Research Conference, 7, California, 29 Feb.-01 Mar. 1972. Descriptors: MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE TIMO TIMO EFEITOS DE RADIAÇAO RATOS ENDOGAMICOS LEW Limits: RATOS Location: BR191.1; WC335.3, 78a

---

page 1 de 1

Refine the search

Database : HANSEN

Advanced form

Search for : Free form

		Search	in field
1			WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type
2	and or and not		WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type
3	and or and not		WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type

Search engine: iAH powered by WWWISIS

IAH - © BIREME/PAHO/WHO Latin American and Caribbean Center on Health Sciences Information