Database : HANSEN Search on : REACAO EM CADEIA DA POLIMERASE/*MT [Subject descriptor] References found : 3 [refine] Displaying: 1 .. 3   in format [Detailed]

page 1 de 1

1 / 3

HANSEN

select to print

Id: 17564 Author: Kramme, Stefanie; Bretzel, Gisela; Panning, Marcus; Kawuma, Joseph; Drosten, Christian Title: Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR ..- Source: s.l; s.n; 2004. 5 p. tab, graf. Abstract: Real-time PCR technology has improved molecular diagnostics of many pathogens, but no such test is available for Mycobacterium leprae. In this report we describe the establishment and the pre-clinical evaluation of such an assay. The test achieved a theoretical analytical sensitivity limit of 194 M. leprae cells per skin biopsy specimen and facilitated quantification of mycobacteria in tissue over a range of 54-54,000,000 cells per sample. In punch skin biopsies from 39 untreated Ugandan patients with newly diagnosed leprosy, the clinical diagnosis could be confirmed in 88.9% of multibacillary and 33.3% of paucibacillary (microscopically negative) patients. Real-time detection thus did not increase the clinical sensitivity of PCR as compared to conventional protocols, in spite of its evidently high analytical sensitivity. On the other hand, as still no culture system exists for M. leprae, the assay appears to be a robust tool for detection of the bacterium in selected clinical situations, as well as for quantitation in experimental settings. (AU). Descriptors: Antígenos de Bactérias/GE Proteínas de Bactérias/GE Biópsia DNA Bacteriano/CH/IP Hanseníase/*DI/MI Dados de Sequência Molecular Mycobacterium leprae/GE/*IP Reação em Cadeia da Polimerase/*MT Sensibilidade e Especificidade Alinhamento de Sequência Análise de Sequência de DNA Pele/MI Uganda Limits: Research Support, Non-U.S. Gov't Humanos Location: BR191.1; 09344/s

2 / 3

HANSEN

select to print

Id: 17311 Author: Montiel, Rafael; Gacia, Carlos; Canadas, Maria P; Isidro, Albert; Guijo, Jose M; Malgosa, Assumpcio Title: DNA sequences of Mycobacterium leprae recovered from ancient bones ..- Source: s.l; s.n; 2003. 2 p. . Descriptors: Osso e Ossos/*MI DNA Bacteriano/*AN Sequências Repetitivas Dispersas Hanseníase/*HI/MI Mycobacterium leprae/*GE/*IP Paleopatologia/MT Reação em Cadeia da Polimerase Mapeamento por Restrição Homologia de Sequência do Ácido Nucleico Espanha Limits: Adulto História da Medicina Medieval Humano Location: BR191.1; 01905/s

3 / 3

HANSEN

select to print

Id: 17276 Author: Qinxue, Wu; Xinyu, Li; Wei, Hou; Tao, Li; Yaoping, Ying; Jinping, Zhang; Xiuling, Cai; Ganyun, Ye Title: A study on PCR for detecting infection with M. leprae ..- Source: s.l; s.n; 1999. 5 p. tab. Abstract: OBJECTIVE: So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point. MATERIALS AND METHODS: Based on the M. leprae gene coding groEL, 65 kD and 16S rRNA, three polymerase chain reactions were developed by using Plikaytis', Woods' and Pattyn's procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose. RESULTS AND CONCLUSION: For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practical purpose, the Woods' PCR optimized by us ought to be chosen firstly. (AU). Descriptors: Estudo Comparativo Primers do DNA DNA Bacteriano/GE Proteína GroEL/GE Hanseníase/*DI/GE/MI Mycobacterium leprae/*GE Mycobacterium tuberculosis/GE Reação em Cadeia da Polimerase/*MT RNA Ribossômico 16S/GE Sensibilidade e Especificidade Limits: Humano Location: BR191.1; 09175/s

---

page 1 de 1

Refine the search

Database : HANSEN

Advanced form

Search for : Free form

		Search	in field
1			WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type
2	and or and not		WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type
3	and or and not		WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type

Search engine: iAH powered by WWWISIS

IAH - © BIREME/PAHO/WHO Latin American and Caribbean Center on Health Sciences Information