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| Id: | 25524
| | Autor: | Patrocinio, Lucas Gomes; Goulart, Isabela Maria Bernardes; Goulart, Luiz Ricardo; Patrocinio, Jose Antonio; Ferreira, Frederico Regerio; Fleury, Raul Negrao
| | Título: | Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction
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| | Fonte: | s.l; s.n; 2005. 6 p. ilus, tab.
| | Resumo: | Several discoveries about leprosy indicate that Mycobacterium leprae transmission mainly accurs by inhalation, and the nose is major port of entry and exit. The present study evaluated the clinical application of PCR for detection of M. leprae DNA in nasal mucosa biopsies in untreated leprosy patients (52) and their contacts (99) from the State Reference Center in Sanitary Dermatology and Leprosy, Uberlandia, MG, Brazil. PCR detection of a 372-base pair DNA fragment from M. leprae was accomplished in 36 (69.2%) patients, from which 34 (91.9%) of them were multibacillaries. Furhermore, PCR was positive in 3 (16.7%) of 18 slit-skin smear negative, 4 (25.0%) of 16 skin lesion BI negative, 8 (33.3%) of 24 nasal mucosa BI negative patients, and 10 of 99 contacts (10.1%). The presence of bacilli in 10.1% of the contats may potentially reflect an occult leprosy, and reflect an occult leprosy, and these patients must be accompanied, followed by a chemoprophylaxy treatment. Considering all PCR results against clinical and BI classification of patients and controls, we have found a sensitivity of 69.2%, a specificity of 89.9% and an accuracy of 82.8%. It has been demonstrated here through PCR of nasal biopsies that the bacillus invades the mucosa, passing through the nasal inferior turbinate to reach peripheral blood. Therefore, the molecular investigation of invasive nasal biopsies by PCR tests has proven to be useful in defining patients of higher risk of transmission and risk-group contacts, which is an important step ti reach the World Health Organization objective towards the elimination of leprosy as a public health problem (AU).
| | Descritores: | MUCOSA NASAL/imunol
MUCOSA NASAL/les
MUCOSA NASAL/microbiol
MUCOSA NASAL/patol
MYCOBACTERIUM LEPRAE/isol
MYCOBACTERIUM LEPRAE/ultraest
REACAO EM CADEIA DA POLIMERASE/estatíst
REACAO EM CADEIA DA POLIMERASE/tend
REACAO EM CADEIA DA POLIMERASE/util
- BIOPSIA/estatíst
BIOPSIA/tend
BIOPSIA/util
HANSENIASE/clas
HANSENIASE/diag
HANSENIASE/microbiol
HANSENIASE/patol
| | Limites: | HUMANO
| | Localização: | BR191.a; 09307/s |
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