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| Id: | 26596
| | Autor: | Wu, Qinxue; Yin, Yueping; Zhang, Liangfen; Chen, Xiaohong; Yu, Yanhau; Li, Zhicheng; Yu, Hua; Lu, Chengzhi; Feng, Suying; Li, Xiaojie; Huo, Wei; Ye, Ganyun. | | Título: | A study on a possibility of predicting early relapse in leprosy using a ND-O-BSA based ELISA.
| | Fonte: | Int. J. Lepr;70(1):1-8, Mar.,2002. tab.
| | Resumo: | Serological methods have been used for detecting infection with Mycobacterium leprae. We have applied a serological test to explore the possibility it could detect a bacterial relapse among patients who have been cured with chemotherapy. More specifically we used an indirect enzyme-linked immunosorbant assay (ELISA) using the natural disaccharide (ND) of the phenolic glycolipid antigen of M. leprae linked to bovine serum albumin as antigen. Antibody levels were measured in sera from normal controls, active leprosy cases, cured leprosy patients, and relapsing leprosy patients. We correlated antibody levels with the type of leprosy, the bacterial index, and with relapse among cured leprosy patients. In our hands, the ND-ELISA, when applied to screening for infection with M. leprae, had excellent sensitivity, specificity, positive and negative predictive values, and both a low false positive rate and a low false negative rate. Antibody levels gradually increased among active patients from the tuberculoid to the lepromatous end of the leprosy spectrum. There was a year-by-year fall in antibody levels in patients responding to chemotherapy. Antibody levels and the bacterial index were correlated using the Spearman's rank correlation method. Serial antibody levels were measured in 666 leprosy patients after being cured with dapsone monotherapy. Over a three year follow up, 95 multibacillary patients became antibody positive and 12 of them had bacterial relapses of their disease. In contrast, among 335 cases that remained antibody negative, only one relapse was seen. Among 44 paucibacillary cured patients who became antibody positive, there was one relapse. There were 192 such patients who remained antibody negative and one relapsed. The risk of relapse is 6.7 times higher among cured multibacillary patients compared to cured paucibacillary patients. Overall, the cumulative relapse rate among antibody positive cases was 13.7%, compared to 0.4% among antibody negative patients. We conclude that the ND-ELISA is a useful tool both for screening for early infection with M. leprae and for predicting a relapse in cured patients, particularly in cured multibacillary patients. (AU)^ien.
| | Descritores: | Hanseníase/imunol
Hanseníase/fisiopatol
ELISA/instrum
ELISA/métodos
| | Limites: | Humanos
| | Meio Eletrônico: | http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n1/v70n1a01.pdf - en.
| | Localização: | BR191.1 |
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