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Id: 26659 Autor: Shimizu, Toshiaki; Tomioka, Haruaki; Matsuoka, Masanori; Sano, Chiaki. Título: Profiles of the mRNA expression by macrophages infected with mycobacterium leprae and mycobacterium avium complex. Fonte: Int. J. Lepr;70(4):250-259, Dec., 2002. ilus, tab. Resumo: In the present study, we examined profiles of the interaction of Mycobacterium leprae and Mycobacterium avium complex (MAC) with murine peritoneal macrophages (M phi s) in terms of up-regulation of M phi expression of proinflammatory and immunosuppressing cytolines (CKs) after infection. First, the reverse transcription polymerase chain reaction (RT-PCR) assay revealed that both MAC and M. leprae infections up-regulated M phi mRNA expression IL-12, TNF-alpha, IL-10, and transformating growth factor-beta (TGF-beta), except that M. leprae-infected M phi s showed no increase in the IL-12 mRNA expression. Second, the RT-PCR assay also showed some differences between M. leprae- and MAC-infected M phi s with respect to the modes of IL-10 and inducible nitric oxide synthase (iNOS) mRNA expression. That is MAC, but not M. leprae, infection caused a prolonged increase in the expression of IL-10 and iNOS mRNAs. Third, a ribonuclease protection assay revealed that M phi s co-infected with MAC and M. leprae showed the Il-12, TNF-alpha and IL-10 mRNA expression in an intermediate mode of those of M phi s infected with either M. leprae or MAC alone. This implies that the CK expression of M. leprae-infected M phi s may be modified by co-infection with MAC. These findings may suggest differential interactions of M. leprae and MAC organisms with murine peritoneal M phi s in terms of the activation of signal transduction pathways for expression of some kinds of immunoregulatory cytokines and immunoprotective enzymes.(AU)^ien. Descritores: RNA/genet RNA/imunol Macrófagos/citol Macrófagos/fisiol Mycobacterium avium/citol Mycobacterium avium/genet Mycobacterium leprae/citol Mycobacterium leprae/genet Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n4/v70n4a02.pdf - en. Localização: BR191.1

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Id: 25575 Autor: Sunderkotter, Cord H; Tomimori-Yamashita, Jane; Nix, Verena; Maeda, Solange M; Sindrilaru, Anca; Mariano, Mario; Sorg, Clemens; Roth, Johannes Título: High expression of myeloid-related proteins 8 and 14 characterizes an inflammatory active but ineffective response of macrophages during leprosy ..- Fonte: s.l; s.n; 2004. 9 p. ilus, tab, graf. Resumo: Macrophages are decisive cells for the course of leprosy as they phagocytose Mycobacterium leprae and have the potential to influence the specific immune response. Expression and release of the myeloid-related protein (MRP) 8 and MRP14 (S100A8 and S100A9) characterize a proinflammatory subtype of macrophage that is prominent in, for example, murine infection with lack of a T helper 1 cell response and in certain highly active chronic inflammations of mice and humans. We investigated cutaneous biopsies of the different forms of leprosy (41 untreated patients) including leprosy reaction type 1 (reversal reaction) and type 2 (erythema nodosum leprosum) (n=18) for expression of MRP8 and MRP14 by subtupes of macrophages. Concomitantly we determined serum levels of MRP8 and MRP14 by sandwich enzyme-linked immunosorbent assay. Expression of MRP8 and MRP14 by CD68-positive macrophages was low in tuberculoid leprosy and rose significantly in borderline tuberculoid leprosy and especially in multibacillary forms, there being expressed by mycobacteria-loaded foam cells. A significant rise of MRP8 and MRP14 expression also occurred in lepra reactions compared to the corresponding non-reactional forms. In type 2 reactions this additional increased was associated with a significant elevation of serum levels. In type 1 it was associated with expression of MRP8 and MRP14 by epitheloid and giant cells, which so far were considered not to express both proteins. In conclusion, we present evidence taht the two prominent proteins MRP8 and MRP14 can be re-expressed in vivo by tissue macrophages in chronic infection, that their increased expression is characteristic for a macrophage subtype associated with high inflammatory but low antimycobacterial activity in the absence of a T helper 1 response, and that their significant rise in serum during erythema nodosum leprosum bears diagnostic and pathophysiological relevance. (AU). Descritores: MYCOBACTERIUM LEPRAE/imunol CELULAS MIELOIDES/citol CELULAS MIELOIDES/imunol - MACROFAGOS/citol  MACROFAGOS/imunol  MACROFAGOS/microbiol  MACROFAGOS/fisiol Limites: HUMANO Localização: BR191.1; 00690/s

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