|
Id: | 22742
| Autor: | Stefani, Mariane M. A; Martelli, Celina M. T; Gillis, Thomas P; Krahenbuhl, James L
| Título: | In situ type 1 cytokine gene expression and mechanism associated with early leprosy progression ..-
| Fonte: | s.l; s.n; 2003. 8 p. ilus.
| Resumo: | We explored the prognostic value of in situ cytokine patterns in 39 patients with single-skin-lesion paucibacillary leprosy before single-dose therapy, with 3 years of follow-up. Interferon (IFN)-gamma, interleukin (IL)-12, IL-10, IL-4, tumor necrosis factor (TNF)-alpha, and macrophage inflammatory protein (MIP)-1alpha mRNA was quantified in skin biopsy samples at diagnosis, and Mycobacterium leprae DNA was detected in 51.4% of cases. Type 1 immunity predominance with measurable IFN-gamma and undetectable IL-4, which is indicative of effective cell-mediated immunity, is compatible with both the reversal reactions (33.3%) and the resolution of lesions (64.1%) observed. A positive correlation between IL-12 and IFN-gamma indicated type 1 polarization via IL-12. The TNF-alpha/MIP-1alpha correlation implied the TNF-alpha induction of chemokines, which is important for granuloma formation. Positive correlations between key regulatory cytokines-IL-10 and IFN-gamma, IL-10 and IL-12, and IL-10 and TNF-alpha-suggests that there may be some level of an intralesional pro- or anti-inflammatory mechanism essential in avoiding immunopathology. (AU).
| Descritores: | Anticorpos Antibacterianos/BL Biópsia Estudos de Coortes Citocinas/BI/*GE/IM Regulação Bacteriana da Expressão Gênica/*IM Hanseníase/DT/*GE/IM Minociclina/TU Mycobacterium leprae/*GE/IM Ofloxacino/TU Prognóstico RNA Mensageiro/BI/GE RNA Viral/BI/GE Reação em Cadeia da Polimerase Via Transcriptase Reversa Rifampina/TU
| Limites: | Adolescente Adulto Criança Feminino Humano Masculino Células Th1/IM
| Localização: | BR191.1; 01884/s |
|