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| Id: | 21230
| | Autor: | Tanimura, Tadayasu; Honda, Hajime; Oshima, Tomoyuki. | | Título: | Studies on the serology of leprosy the complement-fixation reaction by a modified antigen. The Handai methos (Honda).
| | Fonte: | Int. J. Lepr;24(4):434-446, Oct.-Dec. 1956. tab, graf.
| | Resumo: | From the point of departure indicated in the introduction of this report, further studies have been carried out on cardiolipin, cholesterol and cephalin in an effort to obtain an antigen composition with high sensitivity with sera of various types of leprosy but low sensitivity with other sera. Ordinary crude cephalin was fractioned by Folch's method into phosphatidyl-ethanolamine, diphospho-inositide, and phosphatidyl-serine, and these were combined in various ratios. In order to obtain the optimal mixture-ratio of cardiolipin, cholesterol and cephalin, two were kept constatnt and the other changed serially in quantity. The optimal ratio of the three cephalin fractions (in the other stated above) for high sensitivity with pooled sera from lepromatous cases was found to be 29:10:1. The process was repeated with this cephalin mixture and varying quantities of cholesterol and cardiolipin. The least reactive amounts of these various substances were selected as optimal amounts, as prevention of the development of nonspecific reactions was desired. The optimal combination proved to be 0.2 cc. of o.2 per cent cardiolipin 0.2 cc. of 1 per cent cholesterol, and 0.4 cc. of 1 per cent cephalin fractions (0.29 cc. of phosphatidyl-ethenolamine, 0.1 cc. of diphosphoinositide, and 0.01 cc. of phosphatidyl-serine), plus 0.2 cc. of absolute alcohol. The cardiolipin: cholesterol: cephalin ratio of this antigen is 1:5:10...(AU).
| | Descritores: | HANSENIASE
HANSENIASE/compl
HANSENIASE/diag
SEROLOGIA/métodos
| | Meio Eletrônico: | - .
| | Localização: | BR191.1 |
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