Database : HANSEN Search on : TECNICA DE TIPAGEM BACTERIANA [Subject descriptor] References found : 4 [refine] Displaying: 1 .. 4   in format [Detailed]

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Id: 17563 Author: Zhang, Liangfen; Budiawan, Teky; Matsuoka, Masanori Title: Diversity of potential short tandem repeats in Mycobacterium leprae and application for molecular typing ..- Source: s.l; s.n; Oct. 2005. 9 p. tab. Abstract: A recent advance in molecular typing for tracing the transmission of leprosy is the discovery of short tandem repeats (STRs) in Mycobacterium leprae. To substantiate polymorphic loci from STR as promising candidates for molecular typing tools in leprosy epidemiology, 44 STR loci including 33 microsatellites and 11 minisatellites were investigated among 27 laboratory strains by sequencing PCR products. Not all STRs were necessarily polymorphic. Thirty-two out of the 44 loci were polymorphic. Nine polymorphic loci were suitable for identifying genotypes according to the discriminatory capacity, stability, and reproducibility. All the strains were classified into independent genotypes by the selected nine loci. Three multi-case households were subjected to molecular typing. M. leprae obtained from household cases showed identical copy numbers by TTC triplet alone, but the isolates from one family contact case were divided into different genotypes by adding eight other polymorphic loci. The combination of information from multiple loci allows increasing levels of discrimination and it is likely that the generation and documentation of data will result in the choice of a potential molecular typing tool for leprosy epidemiology. (AU). Descriptors: Técnica de Tipagem Bacteriana/* DNA Bacteriano/AN Pé/MI Marcadores Genéticos/*GE Hanseníase/EP/MI Camundongos Endogâmicos BALB C Camundongos Nus Repetições de Microssatélites/GE Repetições Mini-Satélites/GE Mycobacterium leprae/*CL/*GE Análise de Sequência de DNA Sequências Repetidas em Tandem/*GE Variação (Genética)/* Limits: Research Support, Non-U.S. Gov't Camundongos Humanos Animais Location: BR191.1; 09342/s

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Id: 17554 Author: Young, Saroj K; Taylor, G. Michael; Jain, Suman; Suneetha, Lavanya M; Suneetha, Sujai; Lockwood, Diana N. J; Young, Douglas B Title: Microsatellite mapping of Mycobacterium leprae populations in infected humans ..- Source: s.l; s.n; Nov. 2004. 6 p. ilus, mapas, tab. Abstract: To investigate genetic diversity in a bacterial population, we measured the copy numbers of simple sequence repeats, or microsatellites, in Mycobacterium leprae from patients living in and around Hyderabad, India. Three microsatellite loci containing trinucleotide or dinucleotide repeats were amplified from infected tissues, and the copy numbers were established by sequence analysis. Extensive diversity was observed in a cross-sectional survey of 33 patients, but closely related profiles were found for members of a multicase family likely to share a common transmission source. Sampling of multiple tissues from single individuals demonstrated identical microsatellite profiles in the skin, nasal cavity, and bloodstream but revealed differences at one or more loci for M. leprae present in nerves. Microsatellite mapping of M. leprae represents a useful tool for tracking short transmission chains. Comparison of skin and nerve lesions suggests that the evolution of disease within an individual involves the expansion of multiple distinct subpopulations of M. leprae. (AU). Descriptors: Técnica de Tipagem Bacteriana/* Estudos Transversais Família Dosagem de Genes Hanseníase/EP/*MI/*TM Repetições de Microssatélites/*GE Mycobacterium leprae/*CL/*GE Reação em Cadeia da Polimerase Especificidade de Espécies Variação (Genética)/* Limits: HUMANO MASCULINO FEMININO SUPPORT, NON-U.S. GOV'T Location: BR191.1; 09333/s

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Id: 10071 Author: Hughes, M. S; Ball, N. W; Beck, L. A; Lisle, G. W. de; Skuce, R. A; Neill, S. D Title: Determination of the etiology of presumptive felione leprosy by 16S rRNA gene analysis ..- Source: s.l; s.n; 1997. 8 p. ilus, tab. Descriptors: TÉCNICA DE TIPAGEM BACTERIANA SEQUENCIA DE BASES DOENÇAS DO GATO GATOS DNA RIBOSSOMICO GENES BACTERIANOS HANSENIASE HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM AVIUM MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAEMURIUM MYCOBACTERIUM LEPRAEMURIUM MYCOBACTERIUM PARATUBERCULOSIS NOVA ZELANDIA REAÇAO EM CADEIA POR POLIMERASE RNA RIBOSSOMICO 16S HOMOLOGIA DE SEQUENCIA DE AMINOACIDOS Limits: RELATO DE CASO ANIMAL Location: BR191.1; 07036/s

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Id: 9974 Author: Kato, L; Szejtli, J; Szente, L Title: Water soluble complex of palmitic acid in media for cultivation of leprosy-derived psychrophilic mycobacteria from mycobacterium leprae infected tissues ..- Source: s.l; s.n; 1993. 12 p. tab. Descriptors: ANIMAIS TATUS TÉCNICA DE TIPAGEM BACTERIANA MEIOS DE CULTURA TESTES INTRADÉRMICOS HANSENIASE HANSENIASE CAMUNDONGOS MYCOBACTERIUM MYCOBACTERIUM MICOBACTERIOSE MICOBACTERIOSE MYCOBACTERIUM LEPRAE ACIDOS PALMITICOS SOLUBILIDADE TEMPERATURA AMBIENTE Location: BR191.1; 06618/s

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