Database : HANSEN Search on : CAMUNDONGOS [Subject descriptor] References found : 122 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19552 Author: Aarestrup, Fernando Monteiro; Sampaio, Elizabeth Pereira; Moraes, Milton Ozorio de; Albuquerque, Edson C. A; Castro, Ana Paula V; Sarno, Euzenir Nunes. Title: Experimental mycobacterium leprae infection in BALB/c Mice: effect of BCG administration on TNF-a production and granuloma development. Source: Int. J. Lepr;68(2):156-166, Jun., 2000. ilus, graf. Abstract: In the present study, the experimental model of Mycobacterium leprae infection in the foot pads of BALB/c mice was used to investigate the effects of BCG administration on tumor necrosis factor-alpha (TNF-alpha) production and granuloma development. It was observed that mice intravenously infected with BCG 7 months after M. leprae inoculation into the foot pads presented a more effective mycobacteria clearance, revealed by a significant reduction of BCG-colony forming units in the spleen and by the reduction of acid-fast bacilli (AFB) in the foot pads. BCG infection at the peak of M. leprae infection also modulated the granulomatous response to M. leprae by converting mononuclear granulomas into an epithelioid-cell granuloma. Furthermore, lower TNF-alpha serum levels were detected in M. leprae-infected mice when compared to mice infected with M. leprae + BCG. An analysis of the TNF-alpha gene expression in the spleen by semiquantitative reverse transcription-polymerase chain reactions (RT-PCR) demonstrated that co-infection with BCG induced an earlier expression of TNF-alpha mRNA than in M. leprae-infected mice. The numbers of TNF-alpha-positive cells and apoptotic cells were also enhanced in epithelioid versus non-epithelioid granulomas. As a whole, the data suggest that co-infection of M. leprae-infected mice with BCG modulates TNF-alpha synthesis which, in turn, leads to induction of protective epithelioid granuloma formation in the foot pads and subsequent mycobacterial clearance. Macrophage differentiation into epithelioid cells, in association with the enhancement of TNF-alpha production at the granuloma site, may represent a triggering signal that induced apoptosis in these cells, leading to mycobacterial elimination. Moreover, the rate of apoptosis in epithelioid granulomas may well be related to the extent of immunopathologically mediated tissue damage. (AU)^ien. Descriptors: Mycobacterium leprae/imunol Camundongos Endogâmicos BALB C/genet Camundongos Endogâmicos BALB C/imunol Vacina BCG/uso terap Fatores de Necrose Tumoral/genet Fatores de Necrose Tumoral/imunol Limits: Animais Camundongos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2000/pdf/v68n2/v68n2a06.pdf / en Location: BR191.1

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Id: 18425 Author: Belone, A. F. F; Madeira, S; Rosa, P. S; Opromolla, Diltor Vladimir Araujo Title: Experimental reproduction of the Jorge Lobo's disease in BALB/c mice inoculated with Lacazia loboi obtained from a previously infected mouse ..- Source: s.l; s.n; 2001. ^f191^l194 p. ilus, graf. Abstract: Long-term maintenance of Lacazia loboi in the laboratory has not been reported. We report here the use BALB/c mice to maintain the Lacazia loboi for extended period of time. Eight to ten week-old mice were inoculated intradermally in both hind footpads with a fungal suspension from a macerated footpad obtained from an original mouse previous infected with the fungi and sacrificed 8 months after inoculation. The inoculated animals were sacrificed at different time intervals, footpads were excised, the right one was submitted to histopathological examination and the left one was macerated in sterile saline for fungal count and viability index determination. The inoculated animals presented the histopathological picture identical to the mice previously inoculated with material from human lesion. Granulomatous infiltrates with predominance of macrophages and giant cells were observed. The granulomas evolved progressively as observed in the different times of sacrifice. After 7 months of inoculation, macroscopic lesions were observed, and the number of fungi obtained from macerated footpads was higher than the number of inoculated fungi. The pattern of lesion development was similar to what was observed in animals infected with a fungal suspension obtained from a human lesion. Considering the histopathological findings, the clinical manifestations, and the finding of a higher number of fungi obtained than the inoculated into footpads of each mice, we believe the BALB/c mice strain is as an excellent way to amintain L. loboi in laboratory. Moreover, even after serial passages of the funfi, the granulomatous lesions are reproduced consistently in laboratory conditions. (AU). Descriptors: BLASTOMICOSE/clas BLASTOMICOSE/etiol BLASTOMICOSE/imunol BLASTOMICOSE/microbiol BLASTOMICOSE/patol BLASTOMICOSE/vet CAMUNDONGOS ENDOGÂMICOS BALB C/imunol CAMUNDONGOS ENDOGÂMICOS BALB C/microbiol - DOOENÇA DE JORGE LOBO  DOOENÇA DE JORGE LOBO  LACAZIA LOBOI Limits: ANIMAL Location: BR191.1; 08748/s

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Id: 18225 Author: Ebenezer, G. J; Norman, G; Joseph, G. A; Daniel, S; Job, C. K Title: Drug resistant-Mycobacterium leprae--results of mouse footpad studies from a laboratory in south India ..- Source: s.l; s.n; 2002. 12 p. tab. Abstract: Out of 265 biopsies of leprosy patients received at the Experimental Pathology Laboratory of Schieffelin Leprosy Research and Training Centre from 1987 to 1997 for evaluating resistant strains of M. leprae, using the mouse footpad technique, 49 showed resistant strains of M leprae to varying concentrations of dapsone, rifampicin and clofazimine. 23 (47%) of these were from a control area. With 369 skin-smear positive multibacillary (MB) patients as the risk group (denominator), 23 (6.23%) were resistant to one or more drugs. 18 (4.88%) had dapsone resistance, 5 (1.36%) were resistant to rifampicin and 9 (2.44%) had resistance to low concentrations of clofazimine (0.0001%). Out of the 23 biopsies with drug resistance from the control area, primary dapsone resistance was seen in 7 (30%) biopsies and secondary dapsone resistance in 11 (48%). Primary rifampicin resistance was seen in 4 (17.4%) patients, secondary rifampicin resistance in 1 (4.35%) and primary clofazimine resistance in 7 (30%). 3 (13%) of the strains showed secondary clofazimine resistance. One biopsy had resistant strains to all the three drugs. In a control area where properly supervised effective multidrug therapy (MDT) was regularly administered over the years, the emergence of drug resistance is negligible. It may not be the case if the content, duration and regularity of the drug regimen were not satisfactory. Aware of the possible shortcomings in mass administration of MDT, it is emphasized that mouse footpad studies on drug resistance should be made available at least in endemic areas where the incidence of the disease has not changed despite good MDT coverage in order to monitor the emergence of drug resistance. Research into molecular biological identification of drug resistant-M.leprae should be intensified. These steps would help to institute timely measures to check the spread of any drug-resistant organisms in the community. (AU). Descriptors: Clofazimina/PD Dapsona/PD Farmacorresistência Bacteriana Farmacorresistência Bacteriana Múltipla Índia Hansenostáticos/*PD Hanseníase/*DT/MI Camundongos Camundongos Endogâmicos CBA Testes de Sensibilidade Microbiana Mycobacterium leprae/*DE Rifampina/PD Limits: HUMANO ANIMAL MASCULINO FEMININO Location: BR191.1; 09179/S

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Id: 17687 Author: Tapinos, Nikos; Ohnishi, Makoto; Rambukkana, Anura Title: ErbB2 receptor tyrosine kinase signaling mediates early demyelination induced by leprosy bacilli ..- Source: s.l; s.n; 2006. 6 p. ilus, tab, graf. Abstract: Demyelination is a common pathologic feature in many neurodegenerative diseases including infection with leprosy-causing Mycobacterium leprae. Because of the long incubation time and highly complex disease pathogenesis, the management of nerve damage in leprosy, as in other demyelinating diseases, is extremely difficult. Therefore, an important challenge in therapeutic interventions is to identify the molecular events that occur in the early phase before the progression of the disease. Here we provide evidence that M. leprae-induced demyelination is a result of direct bacterial ligation to and activation of ErbB2 receptor tyrosine kinase (RTK) signaling without ErbB2-ErbB3 heterodimerization, a previously unknown mechanism that bypasses the neuregulin-ErbB3-mediated ErbB2 phosphorylation. MEK-dependent Erk1 and Erk2 (hereafter referred to as Erk1/2) signaling is identified as a downstream target of M. leprae-induced ErbB2 activation that mediates demyelination. Herceptin (trastuzumab), a therapeutic humanized ErbB2-specific antibody, inhibits M. leprae binding to and activation of ErbB2 and Erk1/2 in human primary Schwann cells, and the blockade of ErbB2 activity by the small molecule dual ErbB1-ErbB2 kinase inhibitor PKI-166 (ref. 11) effectively abrogates M. leprae-induced myelin damage in in vitro and in vivo models. These results may have implications for the design of ErbB2 RTK-based therapies for both leprosy nerve damage and other demyelinating neurodegenerative diseases. (AU). Descriptors: Anticorpos Monoclonais/PD Butadienos/PD Células COS Células Cultivadas Cercopithecus aethiops Técnicas de Cocultura Doenças Desmielinizantes/*ME/PA Ativação Enzimática/DE Inibidores Enzimáticos/PD Células Hela Hanseníase/*ME/MI Camundongos Knockout Proteína Quinase 1 Ativada por Mitógeno/ME Proteína Quinase 3 Ativada por Mitógeno/ME Mycobacterium leprae/GE/*ME Nitrilos/PD Pirimidinas/PD Pirróis/PD Receptor erbB-2/*ME Research Support, N.I.H., Extramural Células de Schwann/EN/ME Nervo Ciático/ME/MI/UL Transdução de Sinal Limits: Ratos Camundongos Nus Camundongos Humanos Estudo Comparativo Animais Location: BR191.1; 09361/s

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Id: 17563 Author: Zhang, Liangfen; Budiawan, Teky; Matsuoka, Masanori Title: Diversity of potential short tandem repeats in Mycobacterium leprae and application for molecular typing ..- Source: s.l; s.n; Oct. 2005. 9 p. tab. Abstract: A recent advance in molecular typing for tracing the transmission of leprosy is the discovery of short tandem repeats (STRs) in Mycobacterium leprae. To substantiate polymorphic loci from STR as promising candidates for molecular typing tools in leprosy epidemiology, 44 STR loci including 33 microsatellites and 11 minisatellites were investigated among 27 laboratory strains by sequencing PCR products. Not all STRs were necessarily polymorphic. Thirty-two out of the 44 loci were polymorphic. Nine polymorphic loci were suitable for identifying genotypes according to the discriminatory capacity, stability, and reproducibility. All the strains were classified into independent genotypes by the selected nine loci. Three multi-case households were subjected to molecular typing. M. leprae obtained from household cases showed identical copy numbers by TTC triplet alone, but the isolates from one family contact case were divided into different genotypes by adding eight other polymorphic loci. The combination of information from multiple loci allows increasing levels of discrimination and it is likely that the generation and documentation of data will result in the choice of a potential molecular typing tool for leprosy epidemiology. (AU). Descriptors: Técnica de Tipagem Bacteriana/* DNA Bacteriano/AN Pé/MI Marcadores Genéticos/*GE Hanseníase/EP/MI Camundongos Endogâmicos BALB C Camundongos Nus Repetições de Microssatélites/GE Repetições Mini-Satélites/GE Mycobacterium leprae/*CL/*GE Análise de Sequência de DNA Sequências Repetidas em Tandem/*GE Variação (Genética)/* Limits: Research Support, Non-U.S. Gov't Camundongos Humanos Animais Location: BR191.1; 09342/s

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Id: 17562 Author: Kang, Tae Jin; Yeum, Chung Eun; Kim, Byoung Chul; You, Eun-Young; Chae, Gue-Tae Title: Differential production of interleukin-10 and interleukin-12 in mononuclear cells from leprosy patients with a Toll-like receptor 2 mutation ..- Source: s.l; s.n; 2004. 7 p. ilus, tab, graf. Abstract: Toll-like receptor 2 (TLR2) is a key mediator of the immune response to mycobacterial infections, and mutations in TLR2 have been shown to confer susceptibility to infection with mycobacteria. This study investigated the profiles of cytokines, such as interferon (IFN)-gamma, interleukin (IL)-10, IL-12 and tumour necrosis factor (TNF)-alpha in response to Mycobacterium leprae in peripheral blood mononuclear cells (PBMC) with the TLR2 mutation Arg677Trp, a recently reported polymorphism that is associated with lepromatous leprosy. In leprosy patients with the TLR2 mutation, production of IL-2, IL-12, IFN-gamma, and TNF-alpha by M. leprae-stimulated PBMC were significantly decreased compared with that in groups with wild-type TLR2. However, the cells from patients with the TLR2 mutation showed significantly increased production of IL-10. There was no significant difference in IL-4 production between the mutant and wild-type during stimulation. Thus, these results suggest that the TLR2 signal pathway plays a critical role in the alteration of cytokine profiles in PBMC from leprosy patients and the TLR2 mutation Arg677Trp provides a mechanism for the poor cellular immune response associated with lepromatous leprosy. (AU). Descriptors: Sequência de Bases Interleucina-10/*BI Interleucina-12/*BI Hanseníase/GE/*IM Leucócitos Mononucleares/IM Glicoproteínas de Membrana/*GE/ME Camundongos Nus Dados de Sequência Molecular Mutação Puntual/* Receptores da Superfície Celular/*GE/ME Receptor 2 Toll-Like Receptores Toll-Like Fator de Necrose Tumoral alfa/BI Limits: Adulto Idoso Animais Feminino Humanos Masculino Camundongos Research Support, Non-U.S. Gov't Meia-Idade Location: BR191.1; 09341/s

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Id: 17555 Author: Welch, Theodosia M; Gelber, Robert H; Murray, Lydia P; Ng, Herman; O'Neill, Sheila M; Levy, Louis Title: Viability of Mycobacterium leprae after multiplication in mice ..- Source: s.l; s.n; Nov. 1980. 4 p. tab. Abstract: To measure the rate at which Mycobacterium leprae are killed in the course of the mouse footpad infection after the maximum of multiplication has been achieved, M. leprae were harvested shortly before and at intervals after multiplication had reached the level of 10(6) organisms per footpad, serially diluted, and inoculated into the footpads of passage mice. Beginning 1 year later, foot-by-foot harvests of M. leprae were performed from passage mice, and the proportion of viable organisms in the passage inocula was calculated by means of a most-probable-number calculation. In addition, the proportion of solidly staining M. leprae was measured in the passage inocula. The proportion of viable M. leprae in the passage inocula was found to decrease with the time after multiplication to 10(6) organisms per footpad of donor mice; the half-time of loss of viable M. leprae was 25 days. The proportion of solidly staining organisms appeared to be directly related to the proportion of viable organisms, as measured by mouse passage, and inversely proportional to the time after multiplication to 10(6) organisms per footpad. (AU). Descriptors: Hanseníase/IM/*MI Camundongos Endogâmicos BALB C Mycobacterium leprae/*GD/IM Limits: ANIMAL CAMUNDONGOS Research Support, U.S. Gov't, P.H.S. Location: BR191.1; 09334/s

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Id: 13962 Author: Maslov, A. K; Luzhnova, S. A; Kalyanina, O. V Title: Effects of horseradish root on functional activity of phagocytes, total blood cell count, and state of the liver in mice with experimental leprosy ..- Source: s.l; s.n; 2002. 3 p. tab. Abstract: Therapy of experimental leprosy with dried and grated horseradish root administered perorally in a dose of 300 mg/kg mixed food and treatment with purified horseradish peroxidase increased myeloperoxidase activity of blood neutrophils, enhanced antimicrobial functions of phagocytes, decreased leukocytosis, normalized total blood cell count, and produced no adverse effects on the functional state of the liver in mice. (AU). Descriptors: CONTAGEM DE CELULAS SANGUINEAS RELACAO DOSE-RESPOSTA A DROGA HANSENIASE/imunol HANSENIASE/fisiopatol FAGOCITOS/ef drogas FAGOCITOS/imunol CAMUNDONGOS ENDOGÂMICOS CBA FIGADO/ef drogas FIGADO/imunol FIGADO/fisiopatol RAIZES DE PLANTA/quim ARMORACIA/quim Limits: ANIMAL CAMUNDONGOS Electronic Medium: http://www.ilsl.br Location: BR191.1; 09146/s

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Id: 13944 Author: Bochud, Pierre-Yves; Hawn, Thomas R; Aderem, Alan Title: Cutting edge: a toll-like receptor 2 polymorphism that is associated with lepromatous leprosy is unable to mediate mycobacterial signaling ..- Source: s.l; s.n; 2003. 4 p. graf. Abstract: Toll-like receptors (TLRs) are key mediators of the innate immune response to microbial pathogens. We investigated the role of TLRs in the recognition of Mycobacterium leprae and the significance of TLR2Arg(677)Trp, a recently discovered human polymorphism that is associated with lepromatous leprosy. In mice, TNF-alpha production in response to M. leprae was essentially absent in TLR2-deficient macrophages. Similarly, human TLR2 mediated M. leprae-dependent activation of NF-kappaB in transfected Chinese hamster ovary and human embryonic kidney 293 cells, with enhancement of this signaling in the presence of CD14. In contrast, activation of NF-kappaB by human TLR2Arg(677)Trp was abolished in response to M. leprae and Mycobacterium tuberculosis. The impaired function of this TLR2 variant provides a molecular mechanism for the poor cellular immune response associated with lepromatous leprosy and may have important implications for understanding the pathogenesis of other mycobacterial infections. (AU). Descriptors: CELULAS CHO LINHAGEM CELULAR IMUNIDADE NATURAL/genet ARGININA/genet HANSENIASE VIRCHOWIANA/genet HANSENIASE VIRCHOWIANA/imunol HANSENIASE VIRCHOWIANA/microbiol GLICOPROTEINAS DE MEMBRANA/defic GLICOPROTEINAS DE MEMBRANA/genet GLICOPROTEINAS DE MEMBRANA/fisiol CAMUNDONGOS ENDOGÂMICOS C57BL CAMUNDONGOS KNOCKOUT MYCOBACTERIUM LEPRAE/imunol MYCOBACTERIUM TUBERCULOSIS/imunol POLIMORFISMO DE UM UNICO NUCLEOTIDIO/imunol TRANSDUCAO DE SINAL/imunol TRIPTOFANO/genet RECEPTORES DA SUPERFICIE CELULAR/defic RECEPTORES DA SUPERFICIE CELULAR/genet RECEPTORES DA SUPERFICIE CELULAR/fisiol Limits: HUMANO ANIMAL HAMSTERS CAMUNDONGOS SUPPORT, NON-U.S. GOV'T SUPPORT, U.S. GOV'T, P.H.S. Electronic Medium: http://www.ilsl.br Location: BR191.1; 09085/s

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Id: 13790 Author: Hagge, Deanna A; Robinson, Sandra Oby; Scollard, David; McCormick, Gregory; Williams, Diana L Title: A new model for studyng the effects of Mycobacterium leprae on Schwann cell and neuron interactions ..- Source: s.l; s.n; 2002. 14 p. ilus, tab. Abstract: Millions of patients with leprosy suffer from nerve damage resulting in disabilities as a consequence of Mycobacterium leprae infection. However, mechanisms of nerve damage have not been elucidated because of the lack of a model that maintains M. leprae viability and mimics disease conditions. A model was developed using viable M. leprae, rat Schwann cells, and Schwann cell-neuron cocultures incubated at 33 degrees C. M. leprae retained 56 per cente viability in Schwann cells for 3 weeks after infection at 33 degrees C, compared with 3.6 per cente viability at 37 degrees C. Infected Schwann cells had altered morphology and expression of genes encoding cellular adhesion molecules at 33 degrees C but were capable of interacting with and myelinating neurons. Cocultures, infected after myelination occurred, showed no morphological changes in myelin architecture after 1 month of incubation at 33 degrees C, and M. leprae retained 53 per cente viability. This article describes a new model for studying the effects of M. leprae on Schwann cells. (AU). Descriptors: CAMUNDONGOS NUS HANSENIASE/microbiol HANSENIASE/patol MODELOS NEUROLOGICOS MICROSCOPIA ELETRÔNICA DE VARREDURA CELULAS CULTIVADAS NEURÔNIOS/microbiol NEURÔNIOS/patol NEURÔNIOS/fisiol COMUNICACAO CELULAR/fisiol MYCOBACTERIUM LEPRAE/cresc MYCOBACTERIUM LEPRAE/fisiol CELULAS DE SCHWANN/microbiol CELULAS DE SCHWANN/patol CELULAS DE SCHWANN/fisiol CELULAS DE SCHWANN/ultraest Limits: HUMANO ANIMAL CAMUNDONGOS RATOS SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 09030/s

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Id: 13735 Author: Ngamying, Maeya; Sawanpanyalert, Pathom; Butraporn, Raywadee; Nikasri, Junjira; Cho, Sang-Nae; Levy, Louis; Brennan, Patrick J Title: Effect of vaccination with refined components of the organism on infection of mice with Mycobacterium leprae ..- Source: s.l; s.n; 2003. 3 p. tab. Abstract: Only native products of Mycobacterium leprae, whether cell wall, cytosol, or membrane derived, can confer protective immunity against challenge in the mouse footpad. Previously, recombinant proteins were shown to be ineffective. The cell wall skeleton-the mycolyl-arabinogalactan-peptidoglycan complex-devoid of proteins is not protective. (AU). Descriptors: HANSENIASE/prev VACINAS BACTERIANAS/imunol ESQUELETO DA PAREDE CELULAR/imunol CAMUNDONGOS ENDOGÂMICOS BALB C MYCOBACTERIUM BOVIS/imunol MYCOBACTERIUM LEPRAE/imunol VACINACAO Limits: ANIMAL FEMININO CAMUNDONGOS SUPPORT, U.S. GOV'T, P.H.S. Electronic Medium: http://www.ilsl.br Location: BR191.1

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Id: 13530 Author: Hagge, Deanna; Robinson, Sandra Oby; Scollard, David; McCormick, Gregory; Williams, Diana L Title: A new model for studying the effects of Mycobacterium leprae on Schwann cell and neuron interactions ..- Source: s.l; s.n; 2002. 4 p. tab. Abstract: Millions of patients with leprosy suffer from nerve damage resulting in disabilities as a consequence of Mycobacterium leprae infection. However, mechanisms of nerve damage have not been elucidated because of the lack of a model that maintains M. leprae viability and mimics disease conditions. A model was developed using viable M. leprae, rat Schwann cells, and Schwann cell-neuron cocultures incubated at 33 degrees C. M. leprae retained 56% viability in Schwann cells for 3 weeks after infection at 33 degrees C, compared with 3.6% viability at 37 degrees C. Infected Schwann cells had altered morphology and expression of genes encoding cellular adhesion molecules at 33 degrees C but were capable of interacting with and myelinating neurons. Cocultures, infected after myelination occurred, showed no morphological changes in myelin architecture after 1 month of incubation at 33 degrees C, and M. leprae retained 53% viability. This article describes a new model for studying the effects of M. leprae on Schwann cells. (AU). Descriptors: COMUNICACAO CELULAR/fisiol CELULAS CULTIVADAS HANSENIASE/microbiol HANSENIASE/patol CAMUNDONGOS NUS MODELOS NEUROLOGICOS NEURÔNIOS/microbiol NEURÔNIOS/patol NEURÔNIOS/fisiol CELULAS DE SCHWANN/microbiol CELULAS DE SCHWANN/patol CELULAS DE SCHWANN/fisiol CELULAS DE SCHWANN/ultraest MICROSCOPIA ELETRÔNICA DE VARREDURA MYCOBACTERIUM LEPRAE/cresc MYCOBACTERIUM LEPRAE/fisiol Limits: ANIMAL HUMANO CAMUNDONGOS RATOS SUPPORT, NON-U.S. GOV'T Location: BR191.1; 08982/s

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Id: 13527 Author: Lancaster, R. D; McDougall, A. C; Hilson, G. R. F; Colston, M. J Title: Leprosy in the nude mouse ..- Source: s.l; s.n; 1984. 4 p. tab, graf. Abstract: The continued failure to grow the leprosy bacillys in vitro and the limited and localized nature of the infection resulting from the inoculation of Mycobacterium leprae into normal mice emphasise the need for an experimental animal which readily permits growth of the bacillus. (AU). Descriptors: HANSENIASE/fisiopatol CAMUNDONGOS NUS/microbiol MODELOS ANIMAIS DE DOENCAS Limits: ANIMAL CAMUNDONGOS SUPPORT, NON-U.S. GOV'T Location: BR191.1; 08972/s

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Id: 13475 Author: Das, P. K; Ibrahim, A. A; Binkhuysen, F; Thi Hué, P; Qui, T. H; Rangarajan R;; Ruitenberg EJ Title: The use of subcellular components of BCG for studying host-Mycobacterium interaction in relation to leprosy ..- Source: s.l; s.n; Jul.-Aug. 1982. 19 p. ilus, tab, graf. Abstract: Mycobacterium bovis-BCG was sonified and centrifuged at 90,000g for 2h to obtain pellet (P90) and supernatant (S90), and bacilli broken by chilled X-press were fractionated into cell wall (CW), plasma membrane and cytosol. Rabbits were immunized with P90, S90 and whole sonified-broken BCG. The antigenic make-up of these antisera. These analysed by cross-immunoelectrophoresis using the antisera. These subcellular preparations were also used for detecting circulating antibodies in the sera of 45 leprosy patients by Ouchterlony´s technique and immunoelectrophoresis. Although there were many antigenic determinants common to more than one fraction, some components were found in only one fraction. Furthermore, different leprosy patients showed different patterns of antibody response to the antigens in the different fractions. In addition, these fractions were also used to assess cell-mediated immunity in mice sensitized with lyophilized whole BCG as well as with irradiated Mycobacterium. it was found that BCG, intraperitoneally injected, engedered different specifically antigen-sensitized populations of lymphocytes in the spleen and lymph node. Moreover, a certain degree of antigenic cross-reactivity was observed between BCG and M. leprae, possibly at the T-cell level. In vitro experiments suggested that the CW stimulated B cells, indicating a mitogenic ctivity leading to polyclonal activation. Finally, in vitro priming experiments established that these subcellular fractions could sensitize human peripheral lymphocytes, and upon secondary culture all primed cells were able to respond to homologous preparations but not always to heterologous preparations, thus offering a means to distinguish antigens of interest from those antigenically less complicated fractions at the T-cell level.(AU). Descriptors: MYCOBACTERIUM LEPRAE/imunol CAMUNDONGOS ENDOGÂMICOS CBA MYCOBACTERIUM BOVIS/imunol IMUNOELETROFORESE BIDIMENSIONAL HANSENIASE/imunol ANTICORPOS ANTIBACTERIAS/anal ANTIGENOS DE BACTERIAS/anal TATUS FRACOES SUBCELULARES/imunol Limits: HUMANO ANIMAL FEMININO CAMUNDONGOS COELHOS SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 01556/s

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Id: 13451 Author: Young, Douglas B; Khanolkar, Saroj R; Barg, Linda L; Buchanan, Thomas M Title: Generation and characterization of monoclonal antibodies to the phenolic glycolipid of Mycobacterium leprae ..- Source: s.l; s.n; jan. 1984. 6 p. ilus, tab, graf. Abstract: Nine cloned cell lines producing antibodies to the unique phenolic glycolipid of Mycobacterium leprae have been established as a result of fusions with spleens from mice immunized with the glycolipid complexed with methylated bovine serum albumin. One of the antibodies was relatively nonspecific, binding to a related glycolipid from Mycobacterium kansasii, but the remaining antibodies were specific for the M. leprae lipid. Some of the antibodies required the intact (trisaccharide) carbohydrate portion for recognition of the glycolipid antigen, whereas others recognized partially hydrolyzed forms lacking one or two sugar residues. Monoclonal antibodies directed at the terminal saccharide of the glycolipid showed the greatest specificity for M. leprae in enzyme-linked immunoassays. These antibodies brightly labeled whole mycobacteria in indirect immunofluorescence experiments, demonstrating the surface location of M. leprae-specific determinants of the glycolipid antigen. In addition to their use in providing information about the antigenic properties of the phenolic glycolipid, these antibodies have potential applications for elucidating the roles of glycolipid in the pathogenesis of leprosy.(AU). Descriptors: COMPLEXO ANTIGENO-ANTICORPO ELISA IMUNOFLUORESCÊNCIA GLICOLIPIDIOS/anal GLICOLIPIDIOS/imunol MYCOBACTERIUM LEPRAE CAMUNDONGOS ENDOGÂMICOS BALB C FENOIS PLASMOCITOMA/imunol ESPECIFICIDADE DE ESPECIES Limits: ESTUDO COMPARATIVO ANIMAL CAMUNDONGOS SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 01417/s

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Id: 13414 Author: Closs, O; Kronvall, G Title: Experimental murine leprosy ..- Source: s.l; s.n; 1975. 6 p. ilus, tab. Abstract: Sera from C3H and C57BL mice infected with Mycobacterium lepraemurium (MLM) and from human leprosy patients were examined for antibodies against MLM by a crossed immunoelectrophoresis (CIE) technique. Altogether antibodies against six or seven MLM antigens were found in the mouse sera. After a small inoculum of MLM, C3H mice produced more antibodies than C57BL mice. After a large inoculum both strains produced about the same amount of antibodies but showed qualitative differences in their response patterns. A serum pool from patients with lepromatous leprosy contained antibodies against six MLM antigens; five of these were identical with the antigens against which antibodies were found in infected mice. C57BL mice that had lost their delayed-type hypersensitivity to MLM during the course of a disseminated infection showed a fairly strong antibody response to three antigens and a weak response to three others. Since almost the same spectrum of antibodies, although in lower titres, could be demonstrated in C57BL mice with intact delayed-type hypersensitivity to the bacilli, these antibodies did not seem to interfere with the expression of cell-mediated immunity.(AU). Descriptors: MODELOS ANIMAIS DE DOENCAS ANTIGENOS DE BACTERIAS ANTICORPOS ANTIBACTERIAS HIPERSENSIBILIDADE TARDIA/imunol IMUNIDADE CELULAR IMUNOELETROFORESE BIDIMENSIONAL HANSENIASE/imunol CAMUNDONGOS ENDOGÂMICOS C3H/imunol CAMUNDONGOS ENDOGÂMICOS C57BL/imunol MYCOBACTERIUM LEPRAEMURIUM/imunol Limits: HUMANO ANIMAL FEMININO CAMUNDONGOS Electronic Medium: http://www.ilsl.br Location: BR191.1; 00024/s

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Id: 13279 Author: Fieldsteel, A. Howard; Levy, Louis Title: Dapsone chemotherapy of Mycobacterium leprae infection of the neonatally thymectomized Lewis rat ..- Source: s.l; s.n; nov. 1976. 6 p. tab. Abstract: In order to learn whether the neonatally thymectomized Lewis rat (NTLR) infected with Mycobacterium leprae could serve as a model for chemotherapeutic studies in a situation resembling that found in human lepromatous leprosy, NTLR inoculated with M. leprae either locally or intravenously 9 to 16 months earlier were treated for from 1.5 to 8.5 months with dapsone (4,4´-diaminodiphenylsulfone, DDS) incorporated in the rat chow in the concentration providing the minimal inhibitory concentration of the drug for M. leprae and in the 100-fold larger concentration. NTLR were killed at intervals; the M. leprae were counted and passed to mice. Treatment with the smaller dosage of dapsone neither killed M. leprae nor reduced the number of organisms in the bacterial populations, whereas treatment with the larger dosage both killed M. leprae and reduced their numbers. The rate at which the organisms were killed (i.e., rendered noninfective for mice) was much the same as that in patients treated with dapsone in comparable dosage. The dead organisms were removed from the rat tissues at a faster rate than encountered in patients. The NTLR may indeed be suitable for chemotherapeutic studies relevant to man. In addition, the more rapid diappearance of dead M. leprae from the rat tissues may facilitate the study of treatment regimens designed to eradicate persisting viable organisms.(AU). Descriptors: ANIMAIS RECEM-NASCIDOS DAPSONA/admin MODELOS ANIMAIS DE DOENCAS HANSENIASE/quimioter HANSENIASE/parasitol CAMUNDONGOS ENDOGÂMICOS BALB C MYCOBACTERIUM LEPRAE RATOS ENDOGÂMICOS LEW RATOS DE CEPAS ENDOGÂMICAS TIMECTOMIA Limits: ANIMAL MASCULINO FEMININO CAMUNDONGOS RATOS SUPPORT, U.S. GOV'T, P.H.S. Electronic Medium: http://www.ilsl.br Location: BR191.1; 00545/s

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Id: 13220 Author: Rees, R. J; McDougall, A. C;; Weddell, A. G Title: The testis in mice infected with Mycobacterium leprae ..- Source: s.l; s.n; feb. 1975. 7 p. ilus, tab. Abstract: Following inoculation either locally or intravenously with Mycobact. leprae of human origin, the histopathology and bacteriology of the testis in experimental mice is described. Normal mice, and mice rendered immunologically deficient by thymectomy and whole-body irradiation, were studied. Attention is drawn to a heavy bacillation of the testis in mice from both groups. Bacilli were found in and beneath the tunica albuginea, but mainly in interstitial cells and in macrophages surrounding the tubules. The percentage of solidly staining bacilli was high, and globi were frequent. The study showed that the testis in mice is particularly favourable for the lodgement and multiplication of Mycobact. laprae following either local or intravenous inoculation. The significance of this in relation to the metabolism of the leprosy bacillus and to the frequent occurrence of testicular damage in the lepromatous male patient is discussed. This work was supported by grants to A. G. M. Weddell and A. C. McDougall from the Medical Research Council and the British Leprosy Relief Association (LEPRA).(AU). Descriptors: HANSENIASE/microbiol HANSENIASE/patol IMUNOSSUPRESSAO CAMUNDONGOS ENDOGÂMICOS CBA CAMUNDONGOS NUS MYCOBACTERIUM LEPRAE/isol TESTICULO/microbiol TESTICULO/patol TIMECTOMIA IMUNIDADE/ef rad Limits: ANIMAL MASCULINO CAMUNDONGOS Electronic Medium: http://www.ilsl.br Location: BR191.1; 01582/s

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Id: 13217 Author: Portaels, F; Ridder, K. de; Pattyn, S. R Title: Cultivable mycobacteria isolated from organs of armadillos uninoculated and inoculated with Mycobacterium leprae ..- Source: s.l; s.n; Mar.-Apr. 1985. 10 p. tab. Abstract: Mycobacteria were cultivated from 16 out of 32 samples of tissues from armadillos inoculated with Mycobacterium leprae. Three out of 7 samples from non-inoculated armadillos held in captivity were also positive for cultivable mycobacteria. Some isolated strains belonged to the M. avium-intracellulare-scrofulaceum complex, while others were identified as M. gordonae and M. terrae. Unclassified mycobacteria were isolated from M. leprae-inoculated armadillos only. Taxonomic studies confirmed that these new armadillo-derived mycobacteria (ADM) are different from all presently known species of mycobacteria, including M. leprae. The same new species were isolated from different armadillo colonies and were never found in non-inoculated animals. Different factors influence the isolation of these ADM in primary culture. Analysis of some specific markers of these ADM has been suggested in order to quantitatively determine the proportion of these ADM to M. leprae in armadillo tissues. No mycobacteria were cultivated from Nude mouse footpads infected with M. leprae.(AU). Descriptors: TATUS/microbiol HANSENIASE/microbiol CAMUNDONGOS NUS MYCOBACTERIUM/clas MYCOBACTERIUM/cresc MYCOBACTERIUM/isol LINFONODOS/microbiol MYCOBACTERIUM LEPRAE Limits: ANIMAL CAMUNDONGOS SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 02168/s

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Id: 13199 Author: Chandi, S. M; Job, CK Title: The early cellular response to M. leprae ..- Source: s.l; s.n; jul. 1978. 13 p. . Abstract: The ultrastructural changes that develop in mouse peritoneal macrophages from 10 minutes up to 14 weeks after exposure to Mycobacterium leprae are presented. Phagocytosis occurred by a process of engulfment by cytoplasmic processes and incorporation into a phagosome, into which lysosomal enzymes were subsequently introduced. Electron transparent zones (E.T.Z.) were not observed around phagocytosed bacilli in this study, however discrete droplets of lipid-like material appeared in the cytoplasm of macrophages, between 2 and 4 weeks after ingestion of the micro-organisms. Phagosomes with double limiting membranes were observed in macrophages harvested as early as 40 minutes after exposure to M. leprae, contrary to the observations of Evans and Levy (1972).(AU). Descriptors: HANSENIASE/fisiopatol CAMUNDONGOS ENDOGÂMICOS CBA MYCOBACTERIUM LEPRAE/cresc FAGOCITOSE FATORES DE TEMPO MACROFAGOS/microbiol MACROFAGOS/ultraest Limits: ANIMAL CAMUNDONGOS Electronic Medium: http://www.ilsl.br Location: BR191.1; 02143/s

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