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Id: 19872 Author: Stefani, Mariane M. A; Martelli, Celina M. T; Morais-Neto, Otaliba L; Martelli, Pierpaolo; Costa, Mauricio B; Andrade, Ana Lucia S. S de. Title: Assessment of anti-PGL-I as a prognostic marker of leprosy reaction. Source: Int. J. Lepr;66(3):356-364, Sept. 1998. tab, graf. Abstract: The anti-phenolic glycolipid-I (PGL-I) assay as currently applied for leprosy is conceived as an early marker of asymptomatic infection, early disease diagnosis and cure monitoring. Its use as a prognostic marker of reaction is still a matter of controversy. We conducted a case-control study to investigate whether IgM and IgG anti-PGL-I antibodies could discriminate patients at increased risk of developing reactions. Eligible cases were untreated leprosy patients at the onset of type 1 and type 2 reactions recruited from among 600 concurrent, newly detected, untreated leprosy patients attending an outpatient clinic in central Brazil. For the patients with reaction, approximately the same number of leprosy cases without reaction matched as to bacterial index (BI), age and gender were randomly selected. Individuals without clinical leprosy were evaluated as healthy controls. Sera from type 1 reaction (N = 43) and type 2 reaction (N = 26) patients were tested by an ELISA using PGL-I synthetic disaccharide-BSA antigen and 1:300 sera dilution (cut-off point > or = 0.2 OD). Antibody profiles were evaluated by exploratory data analysis and reverse cumulative distribution curves. The IgG anti-PGL-I response did not have a defined pattern, being detected only at low levels. Our results indicate that leprosy patients, independently of their reactional status, produce high levels of IgM anti-PGL-I, demonstrating a strong correlation between the magnitude of antibody response and the BI. Patients with a higher BI were at least 3.4 times more prone to produce an antibody response compared to healthy controls. (AU)^ien. Descriptors: Hanseníase/quimioter Hanseníase/imunol ELISA/instrum Imunoglobulina G/sangue Imunoglobulina M/sangue Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1998/pdf/v66n3/v66n3a06.pdf / en Location: BR191.1

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Id: 19661 Author: Roche, Paul W; Failbus, Sarah S; Britton, Warwick J; Cole, Robert. Title: Rapid method for diagnosis of leprosy by measurements of antibodies to the M. leprae 35-kDa protein: comparison with PGL-1 antibodies detected by ELISA and "Dipstick" methods. Source: Int. J. Lepr;67(3):279-286, Sept., 1999. ilus, graf. Abstract: A new rapid immuno-chromatographic test card for the detection of antibodies to the Mycobacterium leprae 35-kD protein is described. The new assay is compared in the same group of subjects with a direct enzyme ELISA method for 35-kD antibodies and with assays for anti-phenolic glycolipid-I (PGL-I) antibodies using a standard ELISA as well as the recently described [quot ]dipstick[quot ] method. Good concordance was found between the rapid methods and the corresponding ELISA methods. The detection of untreated paucibacillary leprosy by the 35-kD test card was 59% compared with 27% for the PGL-I dipstick; however, the specificity for the 35-kD test card was 90% compared with 100% for the PGL-I dipstick in an endemic population. The potential application of these new, rapid serologic methods for the diagnosis of leprosy under field conditions is discussed. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/fisiol Anexina A3/imunol ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a07.pdf / en Location: BR191.1

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Id: 19245 Author: Wu, Qinxue; Yin, Yueping; Zhang, Liangfen; Chen, Xiaohong; Yu, Yanhau; Li, Zhicheng; Yu, Hua; Lu, Chengzhi; Feng, Suying; Li, Xiaojie; Huo, Wei; Ye, Ganyun. Title: A study on a possibility of predicting early relapse in leprosy using a ND-O-BSA based ELISA. Source: Int. J. Lepr;70(1):1-8, Mar.,2002. tab. Abstract: Serological methods have been used for detecting infection with Mycobacterium leprae. We have applied a serological test to explore the possibility it could detect a bacterial relapse among patients who have been cured with chemotherapy. More specifically we used an indirect enzyme-linked immunosorbant assay (ELISA) using the natural disaccharide (ND) of the phenolic glycolipid antigen of M. leprae linked to bovine serum albumin as antigen. Antibody levels were measured in sera from normal controls, active leprosy cases, cured leprosy patients, and relapsing leprosy patients. We correlated antibody levels with the type of leprosy, the bacterial index, and with relapse among cured leprosy patients. In our hands, the ND-ELISA, when applied to screening for infection with M. leprae, had excellent sensitivity, specificity, positive and negative predictive values, and both a low false positive rate and a low false negative rate. Antibody levels gradually increased among active patients from the tuberculoid to the lepromatous end of the leprosy spectrum. There was a year-by-year fall in antibody levels in patients responding to chemotherapy. Antibody levels and the bacterial index were correlated using the Spearman's rank correlation method. Serial antibody levels were measured in 666 leprosy patients after being cured with dapsone monotherapy. Over a three year follow up, 95 multibacillary patients became antibody positive and 12 of them had bacterial relapses of their disease. In contrast, among 335 cases that remained antibody negative, only one relapse was seen. Among 44 paucibacillary cured patients who became antibody positive, there was one relapse. There were 192 such patients who remained antibody negative and one relapsed. The risk of relapse is 6.7 times higher among cured multibacillary patients compared to cured paucibacillary patients. Overall, the cumulative relapse rate among antibody positive cases was 13.7%, compared to 0.4% among antibody negative patients. We conclude that the ND-ELISA is a useful tool both for screening for early infection with M. leprae and for predicting a relapse in cured patients, particularly in cured multibacillary patients. (AU)^ien. Descriptors: Hanseníase/imunol Hanseníase/fisiopatol ELISA/instrum ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n1/v70n1a01.pdf / en Location: BR191.1

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Id: 18511 Author: Bakker, M. I; Hatta, M; Kwenang, A; Van Mosseveld, P; Faber, W. R; Klatser, P. R; Oskam, L. Title: Risk factores for developing leprosy -A population-based cohort study in Indonesia. Source: In: Bakker, Mirjam.Epidemiology and prevention of leprosy: a cohort study in Indonesia^ien. s.l, The Netherlands Leprosy Relief, 2005. p.106-124^btab, ^bgraf. Abstract: This study identified risk factors for developing leprosy through yearly incidence rates in five island populations. Personal factors, like age, sex, household size and the presence of M.leprae-specific antibodies as well as contact were studied. Of the 94 index patients (patients diagnosed in 2000) 43 (46%) were classified as multibacillary (MB), 17 (19%) were seropositive and 6 (7%) presented M.leprae DNA in nasal swabs as determined by polumerase chain reaction (PCR). All PCR positive patients were also seropositive. Forty-four of the 4903 persons initially without symptoms of leprosy developed leprosy in almost four years follow-up, giving an incidence rate of 2.98 per 1000 person-years. Men had a 2.2 times higher risk (95% Confidence Interval [CI]: 1.2-4.1) to developd leprosy than women. Persons living in households of more than 7 household members. Persons who were seropositive in 2000 had a 3.7 times higher risk (95% CI:1.1-12.4) than seronegative persons. Household contacts of MB patients had an adjusted hazard ratio (aHR) of 4.6 (95% CI:1.6-12.9) and household contacts of PCR positive patients an aHR of 9.36 (95% CI: 2.5-34.9) compared to non-contacts. Patients with PCR positive nasal swabs, suggesting nasal excretion of M.leprae, are probably the patients with the highest transmission patential. Since all index patients who were PCR positive were also seropositive, serology semms an adequate tool to identify these patients. Preventing seropositive persons to become seropositive patients and thus the main source of infection may break the chain of transmission (AU)^ien. Descriptors: HANSENIASE/congen HANSENIASE/compl HANSENIASE/diag ELISA/normas ELISA/util REACAO EM CADEIA DA POLIMERASE/métodos REACAO EM CADEIA DA POLIMERASE/util ANALISE ESTATISTICA Limits: ESTUDO COMPARATIVO Humanos Location: BR191.1; WC335.300, B179e

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Id: 18509 Author: Bakker, M. I; Hatta, M; Kwenang, A; Faber, W. R; Van Beers, S. M; Klatser, P. R; Oskam, L. Title: Population survey to determine risk factors for Mycobacterium leprae transmission and infection. Source: In: Bakker, Mirjam.Epidemiology and prevention of leprosy: a cohort study in Indonesia^ien. s.l, The Netherlands Leprosy Relief, 2005. p.72-86^bmapas, ^btab. Abstract: Background: Not every leprosy patient is equally effective in transmitting Mycobacterium leprae. We studied the spatial distribution of infection (using seropositivity as a marker) in the population to identifity which disease characteristics of leprosy patients are important in transmission. Methods: Clinical data and blood samples for anti-M.leprae ELISA were collected during a cross-sectional survey on five Indonesian islands highly endemic for leprosy. A geographic information system (GIS) was used to define contacts of patients. We investigated spatial clustering of patients and seropositive people and used logist regression to determine risk factors for seropositivity. Results: Of the 3986 people examined for leprosy, 3271 gave blood. Seroprevalence varied between islands (1.7-8.7%) and correlated significantly with leprosy prevalence. Five clusters of patients and two clusters of seropositives were detected. In multivariate analysis, seropositivity significantly differed to be the best discriminator of contact groups with higher seroprevalence: contacts of seropositive patients had an adjusted odds ratio (aOR) of 1.75 (95% CI: 0.92-3,31). This increased seroprevalence was strongest for contact groups living _< 75 metres of two seropositive patients (aOR:3.07;95%CI:1.74-5.42). Conclusions: In this highly endemic area for leprosy, not only household contacts of seropositive patients, but also persons living in the vicinity of seropositive patient were more likely to harbour antibodies against M.leprae. Through measuring the serological status of patients and using a broader definition of contacts, higher risk groups can be more specifically identified (AU)^ien. Descriptors: HANSENIASE/epidemiol MYCOBACTERIUM LEPRAE/cresc ELISA/normas ELISA/util MODELOS LOGISTICOS Limits: ESTUDO COMPARATIVO Humanos Location: BR191.1; WC335.300, B179e

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Id: 18234 Author: Das, Pranab K; Rambukkan, Anura; Baas, J. G; Groothuuis, Dick G; Halperin, Moshe Title: Enzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis ..- Source: s.l; s.n; Feb. 1990. 4 p. ilus, tab. Abstract: Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and 28 tuberculoid leprosy patient reacted in a significant amnner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy pateints, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguihing two polar types of leprosy and for diagnosing leprosy in general (AU). Descriptors: HANSENIASE VIRCHOWIANA/imunol HANSENIASE TUBERCULOIDE/imunol MYCOBACTERIUM TUBERCULOSIS/imunol ELISA/util IMUNOGLOBULINA G/imunol IMUNOGLOBULINA G/ultraest - HANSENIASE/imunol  MICOBACTERIOSE/imunol Limits: HUMANO Location: BR191.1; 09295/s

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Id: 18159 Author: Arruda, Maria Sueli P; *.Coelho, Kunie I.R; *.Montenegro, Mario R*. Title: Experimental paracoccidioidomycosis of hamster inoculated in the cheek pouch ..- Source: Netherlands; s.n; 1994. 7 p. ilus, tab, graf. Abstract: We comparaed the granuloma morphology and immune response of hamsters inoculated with Paracoccidioides brasiliensis (Pb) into the cheek pouch, which lacks lymphatic drainage, and the ffotpad, which is rich in lymphatics. Our objective was to better understand the modulation of Pb granuloma in an immunocompetent animal inoculated in an immunologically privileged site. The humoral immune response (ELISA) and cell mediated immunity (footpad test) became positive on days 7 and 14, respectively in animal inoculated into footpad and on days 35 and 60 in animals inoculated into the pouch. Typical epithelioid granulomas were observed at both sites on day 14. The number of fungi gradually decreased from the beginning of the experiment in footpad lesions, but only after day 35 in pouch granulomas, when cell mediated immunity was detectable. The results indicate that typical epithelioid paracoccidioidomycotic granulomas may develop in the absence of a detectable immune response; however, they are incapable of controlling fungal reproduction. Lack of lymphatic drainage delays the appearance of a detectable immune response, but with time fungi escape from the pouch, elicit an immune response and reach other organs. Our results further indicate the importance of the lymphatics in the pathogenesis of paracoccidioidomycosis. (AU). Descriptors: PARACOCCIDIOIDOMICOSE/compl PARACOCCIDIOIDOMICOSE/diag PARACOCCIDIOIDOMICOSE/microbiol PARACOCCIDIOIDOMICOSE/fisiopatol GRANULOMA/microbiol GRANULOMA/fisiopatol BOCHECHA/anorm BOCHECHA/les BOCHECHA/microbiol - ELISA/instrum  ELISA/métodos  ELISA/tend  ELISA/vet Limits: ESTUDO COMPARATIVO CAMUNDONGOS Location: BR191.1; 09421/S

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Id: 17550 Author: Dugue, Christophe; Perraut, Ronald; Youinou, Pierre; Renaudineau, Yves Title: Effects of anti-endothelial cell antibodies in leprosy and malaria ..- Source: s.l; s.n; Jan. 2004. 9 p. ilus, tab, graf. Abstract: As a result of damaging endothelial cells (ECs), Mycobacterium leprae triggers the production of antibodies (Abs). These anti-EC Abs (AECAs) can be divided into two types. The first type nonspecifically reacts with components of the cytosol (CY) and can be detected by enzyme-linked immunosorbent assay (ELISA). The second specifically reacts with the EC membrane (MB) and requires fluorescence-activated cell sorter (FACS) analysis to be detected. The presence of both types of AECAs was determined in 68 leprosy patients. The ELISA was positive for 35 of them but also for 30 of 34 malaria patients and 17 of 50 healthy African controls. However, whereas FACS analysis showed MB reactivity in only three malaria patients and four controls, this reactivity was found in 27 leprosy patients, more of those having the lepromatous than the tuberculoid form. Specificity for MB, which we failed to absorb by incubation with CY lysates, predominated over that for CY in leprosy, unlike malaria, where the EC reactivity was restricted to the CY. Western blot analysis and two-dimensional electrophoresis revealed that calreticulin, vimentin, tubulin, and heat shock protein 70 were targeted by AECAs from leprosy patients, but other proteins remained unidentified. These auto-Abs, but not those from malaria patients, did activate ECs, as indicated by the E-selectin and intercellular adhesion molecule 1 upregulation, and/or induced them into apoptosis, as documented by four different methods. Our findings suggest that, in some but not all leprosy patients, AECAs may play a role in pathogenesis. (AU). Descriptors: Auto-Anticorpos/*IM Auto-Antígenos/IM Linhagem Celular Citossol/IM Células Endoteliais/*IM/MI/PS ELISA Citometria de Fluxo Hanseníase/*IM/*PP Malária Falciparum/*IM/*PP Limits: HUMANO MASCULINO FEMININO ADOLESCENCIA ADULTO MEIA-IDADE SUPPORT, NON-U.S. GOV'T Location: BR191.1; 09329/s

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Id: 17307 Author: Beyene, D; Aseffa, A; Harboe, M; Kidane, D; MacDonald, M; Klatser, P. R; Bjune, G. A; Smith, W. C. S Title: Nasal carriage of Mycobacterium leprae DNA in healthy individuals in Lega Robi village, Ethiopia ..- Source: s.l; s.n; 2003. 8 p. ilus, tab. Abstract: The number of registered leprosy patients world-wide has decreased dramatically after extensive application of WHO recommended Multiple Drug Therapy (MDT). The annual number of new cases has, however, been almost unchanged in several populations, indicating that the infection is still present at community level. Nasal carriage of Mycobacterium leprae DNA was studied in Lega Robi village in Ethiopia. MDT had been applied for more than ten years, and 718 residents over 5 years old were eligible for the study. During the first survey nasal swab samples were collected from 664 (92.5%) individuals. The results of a Peptide Nucleic Acid-ELISA test for M. leprae DNA interpreted by stringent statistical criteria were available for 589 (88.7%) subjects. Thirty-five (5.9%) individuals without clinical signs of leprosy were positive for M. leprae DNA. Seven PCR positive individuals lived in a household where one or two other members were also positive for M. leprae DNA. During a second survey 8 (46%) of 175 interpretable PNA-ELISA tests were positive. Of 137 individuals tested twice, only two were positive on both occasions whereas 10 were PCR positive only once. The study confirms the widespread distribution of M. leprae DNA in healthy individuals. The feasibility of curbing possible transmission of subclinical infection needs further consideration. (AU). Descriptors: DNA Bacteriano/*AN ELISA Etiópia/EP Mycobacterium leprae/*IP Nariz/*MI Reação em Cadeia da Polimerase Hanseníase/*EP/TM Limits: Adolescente Adulto Idoso Portador/*EP/MI Criança Feminino Humano Masculino Meia-Idade Location: BR191.1. 1946/s

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Id: 13889 Author: Carrazana Hernández, Gonzalo B; Ferrá Torres, Telma M. Title: Resultados de ELISA en la prevalencia de lepra después de monoterapia. Source: Fontilles - Revista de Leprología;17(5):449-457, May.-Ago. 1990. tab. Abstract: Se exponen y analizan los resultados de ELISA en pacientes (89,6 por ciento) de la prevalencia de lepra de 1988, en la ciudad de Camagüey, Cuba, despue´s de nomoterapia. Para realizar ELISA se usó el antígeno específico del M. leprae, el glicolípido fenólico-I. De acuerdo con el tiempo de tratamiento recibido, los resultados se correlacionaron con la clasificación sanitaria y las formas clínicas de la lepra según la clasificación de Madrid. el 77'2 por ciento de los casos multibacilares presentaron seronegatividad en ELISA, y entre ellos, el grupo de pacientes an 10 y más años de tratamiento tuvieron el porcentaje más elevado (46'3 por ciento). La seroposotividad de los casos paucibacilares (15'06 por ciento) se observó fundamentalmente en los primeros 5 años de tratamiento. Los pacientes con lepra lepromatosa tratados 10 y más años resultaron seronegativos en el 54'8 por ciento, pero la positividad persistió en el 12'2 por ciento de estos casos. Los pacientes con lepra dimorfa, tuberculoide e indeterminada bajos porcentajes de positividad (12'1; 13'4 y 14'3 respectivamente) principalmete durante los primeros 5 años de terapia. (AU). Descriptors: HANSENIASE/quimioter HANSENIASE/terap ELISA/ef adv Location: BR191.1

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Id: 13631 Author: Paige, Christopher F; Scholl, Daniel T; Truman, Richard W Title: Prevalence and incidence density of Mycobacterium leprae and trypanosoma cruzi infections within a population of wild nine-banded armadillos ..- Source: s.l; s.n; 2002. 5 p. ilus, tab, graf. Abstract: A total of 415 wild 9-banded armadillos from the East Atchafalaya River Levee (Point Coupee, LA) were collected over 4 years to estimate the incidence and prevalence of Mycobacterium leprae and Trypanosoma cruzi and to discern any relationship between the 2 agents. M. leprae infections were maintained at a high steady prevalence rate year to year averaging 19 per cent. T. cruzi antibody prevalence remained relatively low, averaging 3.9 per cent, and varied markedly between years. Prevalence rates were independent, with only 3 armadillos coinfected with both agents. M. leprae incidence density ranged from 0.47 to 3.5 cases per 1,000 animal-days, depending on case definition, confirming active intense transmission of M. leprae among armadillos. No incident T. cruzi cases were found. These infections seem to occur independently and may be used in comparisons to understand better factors that may influence transmission of these agents. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/anal ANTICORPOS ANTIPROTOZOARIOS/anal TATUS/microbiol DOENCA DE CHAGAS/epidemiol DOENCA DE CHAGAS/vet RESERVATORIOS DE DOENCAS/vet HANSENIASE/epidemiol HANSENIASE/vet INCIDÊNCIA MYCOBACTERIUM LEPRAE/isol DENSIDADE DEMOGRAFICA PREVALÊNCIA ELISA TRYPANOSOMA CRUZI/isol Limits: HUMANO ANIMAL SUPPORT, NON-U.S. GOV'T SUPPORT, U.S. GOV'T, P.H.S. Electronic Medium: http://www.ilsl.br Location: BR191.1; 09000/s

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Id: 13615 Author: Martinuzzo, M. E; Larrañaga, G. F. de; Forastiero, R. R; Pelegri, Y; Fariña, M. H; Alonso, B. S; Kordich, L. C; Carreras, L. O Title: Markers of platelet, endothelial cell and blood coagulation activation in leprosy patients with antiphospholipid antibodies ..- Source: s.l; s.n; 2002. 7 p. tab, graf. Abstract: OBJECTIVE: To evaluate plasma levels of markers of platelet, endothelial cell and blood coagulation activation in leprosy patients with or without antiphospholipid antibodies (aPL) and to compare them to those found in patients with antiphospholipid syndrome (APS). METHODS: 42 patients with leprosy (35 lepromatous and 7 borderline): 29 aPL(+) and 13 aPL(-), as well as 26 healthy subjects as normal controls (NC) and 79 control aPL patients without leprosy (59 with and 20 without APS) were included in the study. Plasma soluble P and E selectin (sPsel and sEsel), and VCAM-1 (sVCAM-1), prothrombin F1 + 2 fragment (F1 + 2), thrombin-antithrombin complexes (TAT) and D dimer (DD) were measured by ELISA. The protein C pathway was assessed by the ProC global test. RESULTS: Leprosy patients with aPL presented increased median levels of sPsel [ng/ml (82.0 vs 36.0, p smaller 0.001)] and sVCAM-1 [ng/ml (495 vs 335, p smaller 0.001)] compared to NC, as observed in control aPL patients without leprosy. Levels of sPsel in aPL(+) patients with leprosy were significantly higher than in aPL(-) ones (52.5 ng/ml), p = 0.005. However, plasma markers of thrombin generation were increased in control aPL patients without leprosy but not in those with leprosy. ProcC global test was abnormal in 24.1 per cent of leprosy patients with aPL compared to 4.4 per cent of NC (p smaller 0.024), and to 57.2 per cent of control patients with aPL without leprosy (p = 0.005). CONCLUSIONS: We demonstrated that although patients with leprosy present a high prevalence of aPL, and platelet and endothelial cell activation in vivo to the same extent than patients with APS, they do not show a procoagulant state. (AU). Descriptors: ANTICORPOS ANTIFOSFOLIPIDEOS/*BL ANTITROMBINA III MARCADORES BIOLOGICOS/BL COAGULACAO SANGUINEA/*PH PLAQUETAS/*PH MOLECULAS DE ADESAO CELULAR/BL ENDOTELIO VASCULAR/*PH ELISA PRODUTOS DE DEGRADACAO DA FIBRINA E DO FIBRINOGENIO/AN GLICOPROTEINAS/IM IMUNOGLOBULINA G/AN IMUNOGLOBULINA M/AN HANSENIASE DIMORFA/*BL HANSENIASE VIRCHOWIANA/*BL INIBIDOR DE COAGULACAO DO LUPUS/BL FRAGMENTOS DE PEPTIDEOS/BL PEPTIDEO HIDROLASES/BL PROTEINA C/AN PROTROMBINA Limits: HUMANO MASCULINO FEMININO ADULTO MEIA-IDADE IDOSO SUPPORT, NON-U.S. GOV'T ADOLESCENTE Location: BR191.1; 09034/s

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Id: 13451 Author: Young, Douglas B; Khanolkar, Saroj R; Barg, Linda L; Buchanan, Thomas M Title: Generation and characterization of monoclonal antibodies to the phenolic glycolipid of Mycobacterium leprae ..- Source: s.l; s.n; jan. 1984. 6 p. ilus, tab, graf. Abstract: Nine cloned cell lines producing antibodies to the unique phenolic glycolipid of Mycobacterium leprae have been established as a result of fusions with spleens from mice immunized with the glycolipid complexed with methylated bovine serum albumin. One of the antibodies was relatively nonspecific, binding to a related glycolipid from Mycobacterium kansasii, but the remaining antibodies were specific for the M. leprae lipid. Some of the antibodies required the intact (trisaccharide) carbohydrate portion for recognition of the glycolipid antigen, whereas others recognized partially hydrolyzed forms lacking one or two sugar residues. Monoclonal antibodies directed at the terminal saccharide of the glycolipid showed the greatest specificity for M. leprae in enzyme-linked immunoassays. These antibodies brightly labeled whole mycobacteria in indirect immunofluorescence experiments, demonstrating the surface location of M. leprae-specific determinants of the glycolipid antigen. In addition to their use in providing information about the antigenic properties of the phenolic glycolipid, these antibodies have potential applications for elucidating the roles of glycolipid in the pathogenesis of leprosy.(AU). Descriptors: COMPLEXO ANTIGENO-ANTICORPO ELISA IMUNOFLUORESCÊNCIA GLICOLIPIDIOS/anal GLICOLIPIDIOS/imunol MYCOBACTERIUM LEPRAE CAMUNDONGOS ENDOGÂMICOS BALB C FENOIS PLASMOCITOMA/imunol ESPECIFICIDADE DE ESPECIES Limits: ESTUDO COMPARATIVO ANIMAL CAMUNDONGOS SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 01417/s

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Id: 13265 Author: Bahr, G. M; Rook, G. A; Moreno, E; Lydyard, P. M; Modabber, F. Z; Stanford, J. L Title: Use of the ELISA to screen for anti-thymocyte and anti-beta 2-microglobulin antibodies in leprosy and SLE ..- Source: s.l; s.n; dec. 1980. 9 p. graf. Abstract: A report is given of the use of the enzyme-linked immunosorbent assay to measure antibody to preparations of human thymocyte membranes (HTMA) and to beta 2-microglobulin. The assay described is simple and rapid, and requires only small quantities of an easily stored membrane preparation. The advantages of this technique over conventional methods involving cytotoxicity are discussed. Raised levels of IgM antibody to beta 2-microglobulin were detected in sera from SLE patients. Raised levels of IgG and IgM antibody to HTMA were found in sera from most active lepromatous cases. Two of eight sera from SLE patients showed raised IgG anti-HMTA, but not raised IgM. An attempt was made to study the subclass of the IgG antibodies found, but when checked against purified human IgG myeloma proteins, the available anti-subclass sera were found to lack the necessary degree of specificity in this assay. (AU). Descriptors: AUTO-ANTICORPOS/anal BETA-GLOBULINAS/imunol ELISA IMUNOGLOBULINA G/anal IMUNOGLOBULINA G/clas IMUNOGLOBULINA M/anal HANSENIASE/imunol LUPUS ERITEMATOSO SISTÊMICO/imunol LINFOCITOS T/imunol MICROGLOBULINA-2 BETA/imunol Limits: HUMANO SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 0713/s

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Id: 13190 Author: Saad, M. H; Medeiros, M. A; Gallo, M. E; Gontijo, P. P; Fonseca, L. S Title: IgM immunoglobulins reacting with the phenolic glycolipid-1 antigen from Mycobacterium leprae in sera of leprosy patients and their contacts ..- Source: s.l; s.n; Apr.-Jun. 1990. 4 p. tab. Abstract: For the first time in Brazil it was investigated the occurrence of IgM anti-PGL-1 in the sera of household contacts of leprosy patients using the ELISA methodology. The sera of the multibacillary patients showed significantly more immuno-reactivity than from the paucibacillary patients. It was observed a high subclinical infection incidence among household contacts (19.4%). The percentage of leprosy development was 5% (1/21) among the seropositive contact group. This finding suggests that serology could be useful as prognostic test, but for better definition is necessary to test a population from endemic area for long period time.(AU). Descriptors: ELISA SEGUIMENTOS GLICOLIPIDIOS/anal HANSENIASE/diag HANSENIASE/imunol IMUNOGLOBULINA M/anal MYCOBACTERIUM LEPRAE/imunol PROGNOSTICO Limits: HUMANO ADOLESCENTE ADULTO IDOSO CRIANCA FEMININO MASCULINO MEIA-IDADE Electronic Medium: http://www.ilsl.br Location: BR191.1; 02104/s

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Id: 12230 Author: Salotra, P; Sreenivas, G; Nasim, A. A; Subba Raju, B. V; Ramesh, V Title: Evaluation of enzyme-linked immunosorbent assay for diagnosis of post-kala-azar dermal leishmaniasis with crude or recombinant k39 antigen ..- Source: s.l; s.n; Mar. 2002. 4 p. tab, graf. Descriptors: ANTICORPOS ANTIPROTOZOARIOS ELISA ESTUDOS DE AVALIAÇAO LEISHMANIA DONOVANI LEISHMANIA DONOVANI LEISHMANIA DONOVANI LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL PROTEINAS DE PROTOZOARIOS SENSIBILIDADE E ESPECIFICIDADE Limits: ANIMAL Location: BR191.1; 08713/s

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Id: 11641 Author: Salotra, P; Sreenivas, G; Nasim, A. A; Subba Raju, B. V; Ramesh, V Title: Evaluation of enzyme-linked immunosorbent assay for diagnosis of post-kala-azar dermal leishmaniasis with crude or recombinant k39 antigen ..- Source: s.l; s.n; 2002. 4 p. tab, graf. Descriptors: HANSENIASE LEISHMANIOSE ELISA Location: BR191.1; 08502/s

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Id: 11567 Author: Larrañaga, G. F. de; Forasteiro, P. R; Martinuzzo, M. E; Carreras, L. O; Tsariktsain, G; Sturno, M. M; Alonso, B. S Title: High prevalence of antiphospholipid antibodies in leprosy: evaluation of antigen reactivity ..- Source: s.l; s.n; 2000. 7 p. tab, graf. Descriptors: ADOLESCENCIA ANTICORPOS DE ANTICARDIOLIPINA ANTICORPOS ANTIFOSFOLIPIDIOS AUTO-ANTICORPOS ELISA GLICOPROTEINAS HANSENIASE HANSENIASE PROTROMBINA IMUNOGLOBULINA G/BL IMUNOGLOBULINA M/BL Location: BR191.1; 08403/s

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Id: 11466 Author: Cho, Sang-Nae; Cellona, Roland V; Villahermosa, Laarni G; Fajardo Junior, Tranquilino T; Balagon, M. Victoria F; Abalos, Rodolfo M; Tan, Esterlina V; Walsh, Gerald P; Kim, Joo-Deuk; Brennan, Patrick J Title: Detection of phenolic glycolipid I of mycobacterium leprae in sera fron leprosy patients before and after start of multidrug therapy ..- Source: s.l; s.n; 2001. 5 p. ilus, tab, graf. Descriptors: ANTIGENOS DE BACTÉRIAS ELISA GLICOLIPIDIOS LEPROSTATICOS HANSENIASE HANSENIASE HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE ESTUDOS RETROSPECTIVOS Location: BR191.1; 08345/s

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Id: 11463 Author: Chatterjee, Mitali; Jaffe, Charles L; Sundar, Shyam; Basu, Debasis; Sen, Sandeep; Mandal, Chitra Title: Diagnostic and prognostic potential of a competitive enzyme-linked immunosorbent assay for leishmaniasis in India ..- Source: s.l; s.n; 1999. 5 p. ilus, tab, graf. Descriptors: ANTICORPOS MONOCLONAIS ANTICORPOS ANTIPROTOZOARIOS GLUCONATO DE ANTIMONIO E SODIO ANTIPROTOZOARIOS REAÇOES CRUZADAS ELISA INDIA LEISHMANIA DONOVANI LEISHMANIOSE CUTANEA LEISHMANIOSE CUTANEA LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL HANSENIASE HANSENIASE PROGNOSTICO MALARIA MALARIA TUBERCULOSE TUBERCULOSE Limits: ANIMAL Location: BR191.1; 08342/s

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