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| Id: | 18643
| | Autor: | Hagge, Deanna A; Robinson, Sandra Oby; Scollard, David; McCormick, Gregory; Williams, Diana L
| | Título: | A new model for studyng the effects of Mycobacterium leprae on Schwann cell and neuron interactions
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| | Fonte: | s.l; s.n; 2002. 14 p. ilus, tab.
| | Resumo: | Millions of patients with leprosy suffer from nerve damage resulting in disabilities as a consequence of Mycobacterium leprae infection. However, mechanisms of nerve damage have not been elucidated because of the lack of a model that maintains M. leprae viability and mimics disease conditions. A model was developed using viable M. leprae, rat Schwann cells, and Schwann cell-neuron cocultures incubated at 33 degrees C. M. leprae retained 56 per cente viability in Schwann cells for 3 weeks after infection at 33 degrees C, compared with 3.6 per cente viability at 37 degrees C. Infected Schwann cells had altered morphology and expression of genes encoding cellular adhesion molecules at 33 degrees C but were capable of interacting with and myelinating neurons. Cocultures, infected after myelination occurred, showed no morphological changes in myelin architecture after 1 month of incubation at 33 degrees C, and M. leprae retained 53 per cente viability. This article describes a new model for studying the effects of M. leprae on Schwann cells. (AU).
| | Descritores: | CAMUNDONGOS NUS
HANSENIASE/microbiol
HANSENIASE/patol
MODELOS NEUROLOGICOS
MICROSCOPIA ELETRÔNICA DE VARREDURA
CELULAS CULTIVADAS
NEURÔNIOS/microbiol
NEURÔNIOS/patol
NEURÔNIOS/fisiol
COMUNICACAO CELULAR/fisiol
MYCOBACTERIUM LEPRAE/cresc
MYCOBACTERIUM LEPRAE/fisiol
CELULAS DE SCHWANN/microbiol
CELULAS DE SCHWANN/patol
CELULAS DE SCHWANN/fisiol
CELULAS DE SCHWANN/ultraest
| | Limites: | HUMANO
ANIMAL
CAMUNDONGOS
RATOS
SUPPORT, NON-U.S. GOV'T
| | Meio Eletrônico: | - .
| | Localização: | BR191.1; 09030/s |
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