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| Id: | 13274
| | Author: | Rasbridge, Marian M; Scott, G. L
| | Title: | The haemolytic action of dapsone: changes in the red-cell membrane
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| | Source: | s.l; s.n; feb. 1973. 11 p. tab, graf.
| | Abstract: | Some aspects of red-cell membrane structure and function have been investigated in patients with haemolysis due to dapsone (4,4' diaminodiphenylsulfone). Osmotic fragility and potassium flux were not increased but red-cell membrane changes occurred which suggest instability. These included loss of phospholipid, decrease in acetylcholinesterase activity and increased autohaemolysis. In addition, there was evidence for lipid peroxidation, which might have been the primary lesion. Red-cell membrane sulphydryl group activity was either reduced or altered because these cells were abnormally sensivite to the action of the sulphydryl group inhibitor p-chloromercuribenzoate, as measured by increased potassium leakage and haemolysis. Dapsone also caused abnormal peroxide lysis in the presence of normal hydrogen peroxide detoxification mechanisms and in the absence of vitamin E deficiency. This was prevented by excess vitamin E but not by the sulphydryl compounds, reduced glutathione or cysteine, and it was increased by previous treatment with p-chloromercuribenzoate. It is suggested that the reduced membrane sulphydryl group activity, probably due to the formation of mixed disulphides with precipitated haemoglobin, and lipid proxidation may be responsible for in vitro and, possibly, in vivo dapsone-induced haemolysis.(AU).
| | Descriptors: | ACETILCOLINESTERASE/sangue
MEMBRANA CELULAR/ef drogas
DAPSONA/ef adv
DERMATITE HERPETIFORME/quimioter
HEMOLISE/ef drogas
LIPIDIOS/metab
FRAGILIDADE OSMOTICA
PEROXIDOS/metab
FOSFOLIPIDIOS/sangue
COMPOSTOS DE SULFIDRILA
| | Limits: | MASCULINO
FEMININO
MEIA-IDADE
| | Electronic Medium: | http://www.ilsl.br
| | Location: | BR191.1; 01857/s |
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