Database : HANSEN Search on : RECEPTORES DO FATOR DE CRESCIMENTO TRANSFORMADOR BETA/IMUNOL [Subject descriptor] References found : 2 [refine] Displaying: 1 .. 2   in format [Detailed]

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Id: 13951 Author: Kiszewski, C. A; Becerril, E; Baquera, J; Aguilar, L. D; Hernández-Pando, R Title: Expression of transforming growth factor-beta isoforms and their receptors in lepromatous and tuberculoid leprosy ..- Source: s.l; s.n; 2003. 7 p. ilus, tab, graf. Abstract: Leprosy is an infectious disease with two polar forms, tuberculoid leprosy (TT) and lepromatous leprosy (LL), that are characterized by strong cell-mediated immunity (CMI) and CMI anergy, respectively. Transforming growth factor-beta (TGF-beta) belongs to a family of pleiotropic cytokines (TGF-beta1, TGF-beta2 and TGF-beta3) that participate in the control of cell differentiation and proliferation, as well as tissue repair. This cytokine family is unique because it suppresses CMI. In this study, we compared the expression of the three TGF-beta isoforms and their receptors in skin biopsies from LL and TT patients (LL = 20; TT = 20) using immunohistochemistry and automated morphometry. The percentage of cells immunostained for the three TGF-beta isoforms and cells positive for the three TGF-beta receptors in the inflammatory infiltrate located in the papillary dermis, reticular dermis and periadnexal tissue were significantly higher in LL than that in TT, with macrophages being the most common and strongest immunoreactive cells. Some lymphocytes, fibroblasts, keratinocytes and epithelial cells from sweat glands and hair roots were also positive. In situ reverse-transcription polymerase chain reaction corroborated the capacity of these cells to synthesize TGF-beta1 and TGF-beta receptor 2. This high expression of TGF-beta isoforms and their receptors could contribute to CMI anergy and other clinical characteristic features of leprosy, like skin atrophy. (AU). Descriptors: HANSENIASE VIRCHOWIANA/imunol HANSENIASE VIRCHOWIANA/metab HANSENIASE TUBERCULOIDE/imunol HANSENIASE TUBERCULOIDE/metab MYCOBACTERIUM LEPRAE ISOFORMAS DE PROTEINAS HIBRIDIZACAO IN SITU RNA MENSAGEIRO/quim RNA MENSAGEIRO/genet RECEPTORES DO FATOR DE CRESCIMENTO TRANSFORMADOR BETA/bios RECEPTORES DO FATOR DE CRESCIMENTO TRANSFORMADOR BETA/imunol PELE/citol PELE/imunol REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE REVERSA IMUNOHISTOQUIMICA BIOPSIA FATOR DE CRESCIMENTO TRANSFORMADOR BETA/bios FATOR DE CRESCIMENTO TRANSFORMADOR BETA/genet FATOR DE CRESCIMENTO TRANSFORMADOR BETA/imunol Limits: HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09092/s

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Id: 7961 Author: Goulart, Isabela Maria Bernardes Title: Produçao de TGF-B1 por células mononucleares do sangue periférico de portadores de hanseníase e seu papel na regulaçao da resposta inflamatória ?- Source: Ribeirao Preto; s.n; 1999. 155 p. ilus, tab, graf. Thesis: Present for Universidade de Sao Paulo. Faculdade de Medicina de Ribeirao Preto presented for the degree Doutor. Abstract: Investigou-se, no presente trabalho, os níveis de TGF-B1 em sobrenadantes de cultura de células aderentes, obtidas de células mononucleares do sangue periférico de voluntários sadios e pacientes com hanseníase, virgens de tratamento. Estas células foram separadas pelo Ficoll-Hypaque após remoçao das plaquetas por lavagem em tampao Versene e centrifugaçao diferencial. Os monócitos foram aderidos em placas de cultura em presença de meio RPMI 1640 suplementado com soro humano AB inativo a 2,5 por cento e cultivados, posteriormente, em meio livre de soro, na presença de LPS ou PGL-1. O TGF-B1 foi mensurado por Elisa nos sobrenadantes colhidos após 48 horas de cultura. Demonstrou-se que as células aderentes dos doentes e dos controles(AU). Descriptors: HANSENIASE HANSENIASE RECEPTORES DO FATOR DE CRESCIMENTO TRANSFORMADOR BETA Location: BR191.1; WC335.500, G729p

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