Base de dados : HANSEN Pesquisa : CITOCINAS [Descritor de assunto] Referências encontradas : 73 [refinar] Mostrando: 1 .. 20   no formato [Detalhado]

página 1 de 4

ir para página

1 / 73

HANSEN

seleciona para imprimir

Id: 27283 Autor: Yogi, Yasuko; Nomaguchi, Hiroko; Sakamoto, Yoshiki; Matsuoka, Masanori; Fujimura, Takao; Okamura, Haruki; Hioki, Kyoji; Saito, Muneo; Nomura, Tatsuji. Título: Cytokine gene expression in the foot pad and spleen of BALB/cAJcI mice infected with M. leprae. Fonte: Int. J. Lepr;65(1):80-89, Mar., 1997. ilus, tab. Resumo: The cytokine mRNAs expressed in the foot pads and spleens of BALB/cAJcl mice infected with Mycobacterium leprae were studied by the reverse transcriptase-polymerase chain reaction (RT-PCR) method using cytokine-specific primers for interleukin-1 alpha (IL-1 alpha), -2, -4, -6, -10, -12-(p40), gamma interferon (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and TNF-beta, and then for CD4 and CD8 markers. The pattern of cytokine gene expression in the foot pad which supports M. leprae growth was different from the expression in the spleen which does not permit M. leprae multiplication in mice. Before BALB/cAjcl mice were infected with M. leprae, IL-1 alpha and TNF-beta mRNAs were expressed physiologically in the foot pad while all of the cytokine genes examined were expressed in the spleen. In the foot pads of mice inoculated with M. leprae, in addition to the physiological appearance of IL-1 alpha and TNF-beta mRNAs, these signals were intensified. TNF-alpha expression was induced by the infection. On the other hand, in the spleens of mice inoculated with M. leprae, CD4 mRNA expression disappeared on day 1 of the infection, which was accompanied by the reduced expression of IL-2, -4, -6, and -12 mRNAs. The recovery of CD4 mRNA expression at a latter stage was accompanied by a corresponding increase of the cytokine mRNA expression. It was suspected that these results might permit restricted growth of M. leprae in the foot pads of normal mice. Furthermore, our study suggests that tissue-specific, local, immunologic characteristics are important in M. leprae growth. (AU)^ien. Descritores: Citocinas/bios Citocinas/genet Mycobacterium leprae/imunol Localização: BR191.1

2 / 73

HANSEN

seleciona para imprimir

Id: 27281 Autor: Nakamura, Chikako; Fukutomi, Yasuo; Kashiwabara, Yoshiko; Oomoto, Yasukazu; Kojima, Miyae; Hayashi, Hidetoshi; Onozaki, Kikuo. Título: Dharmendra antigen but not integral M. leprae is an efficient inducer of immunostimulant cytokine production by human monocytes, and M. leprae lipids inhibit the cytokine production. Fonte: Int. J. Lepr;65(1):63-72, Mar., 1997. tab. Resumo: Killed integral Mycobacterium leprae, Mitsuda antigen, and chloroform-treated M. leprae, Dharmendra antigen (Dh-Ag), have been used for the classification of leprosy patients based on cell-mediated immunity. Heat-killed M. leprae also were used as a component of the Convit vaccine. Human blood monocytes were stimulated with M. leprae or Dh-Ag and their cytokine-inducing ability was compared. Monocytes were cultured in the presence of fresh human serum because of the efficiency of cytokine induction and the phagocytosis of M. leprae have been shown to be optimal in the presence of fresh serum. M. leprae and Dh-Ag were equally phagocytosed by monocytes. Dh-Ag was more potent than M. leprae in the induction of immunostimulatory/proinflammatory cytokines, interleukin-1 (IL-1), IL-6 and tumor necrosis factor (TNF). In contrast, a comparable level of IL-1ra, an immunosuppressive cytokine, was induced by M. leprae and Dh-Ag. The lipids extracted from M. leprae induced none of these cytokines by monocytes. Nevertheless, when monocytes were pretreated with the lipids followed by stimulation with Dh-Ag, productions of IL-1, IL-6 and TNF were all inhibited in a dose-dependent manner. However, the lipids did not inhibit the cytokine production induced by other stimuli including BCG and lipopolysaccharide. Moreover the lipids did not affect the production of IL-1ra. These results suggest that the lipids from M. leprae are responsible for the poor cytokine-inducing ability of M. leprae, thus favoring their infection. These results also suggest that Dh-Ag rather than integral M. leprae may be useful as a vaccine candidate because Dh-Ag is able to induce a large amount of cytokines from monocytes. (AU)^ien. Descritores: Citocinas/metab Monócitos/imunol Monócitos/metab Mycobacterium leprae/imunol Localização: BR191.1

3 / 73

HANSEN

seleciona para imprimir Texto Completo-en

Id: 26737 Autor: Shabaana, Abdul Khader; Venkatasubramani, Rajendran; Shanker Narayan, Nallakandypanangadan; Hoessli, Daniel C; Dharmalingam, Kuppamuthu. Título: Cytokine profiles in paraffin-embedded biopsy samples of lepromatous leprosy patients: semi-quantitative measure of cytokine mRNA using RT-PCR. Fonte: Int. J. Lepr;69(3):204-214, Sept., 2001. ilus, tab. Resumo: A reproducible technique for fixation of tissue, RNA extraction and reverse transcription polymerase chain reaction (RT-PCR) analysis from paraffin-embedded leprosy biopsies, has been developed and used to study the mRNA profiles. This approach is valuable in retrospective analysis of gene expression, and the handling of infectious biopsy material is also minimized. Among the methods of RNA extraction compared, the most efficient method was found to be incubation of the tissue sections in digestion buffer followed by extraction with Trizol. The experimental conditions were optimized for first strand cDNA synthesis and PCR, and used to measure the quantity of cytokine transcripts in biopsy materials. Interleukin-10 (IL-10) mRNA was detectable in all cases examined, which correlates well with other earlier reports using frozen tissues. However, IL-5 transcripts were present in 60% of the biopsies, unlike the earlier reports which showed IL-5 mRNA in all LL cases. Transforming growth factor-beta (TGF-beta) mRNA was detected in 80% of the biopsies, and this confirmed earlier immuno-cytochemical data which showed TGF-beta protein in all cases. Tumor necrosis factor-alpha mRNA was found in about 60% of the biopsies; whereas interferon gamma mRNA was detected in 30% of the LL cases. In conclusion, the results obtained in our study confirm and extend earlier observations which examined cytokines in peripheral blood cells and dermal lesions of leprosy. The simplicity of this method would allow the examination of a large number of samples across the spectrum of leprosy.(AU)^ien. Descritores: Hanseníase Virchowiana/genet Hanseníase Virchowiana/imunol Hanseníase Virchowiana/fisiopatol Citocinas/genet RNA Mensageiro/genet Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos Limites: Humanos Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n3/v69n3a04.pdf - en. Localização: BR191.1

4 / 73

HANSEN

seleciona para imprimir

Id: 25642 Autor: Venturini, James Título: Hanseniase Dimorfa: perfil de citocinas em sobrenadante de cultura de células mononucleares e in situ Leprosy bordeline: profile of cytokines in the culture supernatant of mononuclear cells and in situ- Fonte: Botucatu; s.n; 2008. 180 p. ilus, tab, graf. Tese: Apresentada a Universidade Estadual Paulista. Faculdade de Medicina de Botucatu para obtenção do grau de Mestre. Resumo: Visando contribuir para o melhor entendimento da participação das citocinas na hanseníase dimorfa, o presente estudo investigou a produção desses mediadores in vitro e in situ em pacientes dimorfos-tuberculóides (HDT) e dimorfos-virchovianos(HDV). Foram avaliados 7 pacientes HDT e 12 HDV, virgens de tratamento, além de 19 indivíduos sádios (grupo controle). Culturas de células mononucleares do sangue periférico (PBMC) foram estimuladas ou não com estímulos inespecíficos e específicos do M. Leprae (antígeno inteiro e sonicado) e após 48 horas o sobrenadante foi recolhido para dosagens das citocinas TNF-a, IFN-y, IL-10 e TGF-B1. Biópsias das lesões cutâneas foram submetidas aos procedimentos histológicos por meio da coloração com hematoxilina-Eosina e Faraco-Fite; os cortes foram submetidos, ainda, à detecção in situ de iNOS, IL-10 e TGF-B1 por imunoistoquímica. A quantificação de citocinas em sobrenadante de PBMC revelou que pacientes HDT produziram níveis menores de TGF-B1 e pacientes HDV, níveis menores de IL-10...(AU)^ipt. Descritores: Hanseníase Dimorfa/imunol Hanseníase Dimorfa/patol Técnicas de Cultura de Células/métodos Citocinas/bios Citocinas/imunol - Interleucina-10/bios  Interleucina-10/imunol Limites: Humanos Localização: BR191.1; WC335.102.32, V568h

5 / 73

HANSEN

seleciona para imprimir

Id: 24510 Autor: Foss, Norma T. Título: Cytokines profile in the serum and in the spernatant of cultures of PMNBC from the leprosy patients. Fonte: Hansen. int;(n.esp):143-143, Jun. 1998. . Conferência: Apresentado em: Congresso da Associação Brasileira de Hansenologia, IX, Foz do Iguaçu, 04-08 junho 1997. Descritores: CITOCINAS/anal CITOCINAS/quim CITOCINAS/farmacol CITOCINAS/fisiol HANSENIASE/quimioter HANSENIASE/imunol HANSENIASE/fisiopatol Limites: RELATO DE CASO ESTUDO COMPARATIVO HUMANO Localização: BR 191.1

6 / 73

HANSEN

seleciona para imprimir Texto Completo-pt

Id: 23236 Autor: Barreto, Jaison Antonio Título: Hanseniase dimorfa reacional: estudo comparativo, em biopsias cutaneas, entre reacao tipo 1 ocorridas antes e durante a poliquimioterapia ?- Fonte: Sao Paulo; s.n; 2005. [107] p. ilus, tab. Tese: Apresentada a Sao Paulo (Estado). Secretaria de Estado da Saude. Coordenadoria de Controle de Doencas para obtenção do grau de Mestre. Resumo: A hanseniase dimorfa e a forma clinica mais frequentemente associada a ocorrencia de reaçoes de hipersensibilidade medida por celulas (reacao tipo 1), que podem ocorrer antes, durante ou depois do tratamento especifico. Ha varias teorias a respeito da patogenese dessas reacoes, as quais estao diretamente ligadas ao dano neural e as sequelas. Visando-se compreender melhor a fisiopatologia das reacoes tipo 1, foram estudadas 10 biopsias cutaneas de individuos com hanseniase dimorfa-tuberculoide reacional nao tratados e 10 de individuos dimorfos em reacao reversa (apos o inicio do tratamento especifico), comparando-se os parametros morfologicos e imunoistoquimicos. Observou-se no grupo em tratamento, maior positividade das celulas para a enzima oxida nitrico sintase induzivel (iNOS) e menor quantidade de linfocitos T CD8+(p menor 0,05). Nao houve diferenças significativas na baciloscopia e positividade para antigenos micobacterianos nos dois grupos, e nem na quantidade de celulas IL-10+, apesar de ter sido observada correlaçao negativa entre esta citocina e a proporçao CD4/CD8 nos pacientes em tratamento (p menor 0,05). Notou-se tambem, tendencia a reduçao do infiltrado especifico (linfocitos T e B) e aumento do numero de celulas citotoxicas inespecificas (NK) no grupo em tratamento. Estes resultados sao concordantes com trabalhos recentes, que surgerem que a reaçao tipo 1 representaria um desiquilibrio imunologico entre citocinas pro-inflamatorias e anti-inflamatorias...(AU). Descritores: HANSENIASE DIMORFA ANTIBIOTICOS COMBINADOS GRANULOMA CONTAGEM DE CELULAS CITOCINAS SINTASE DE OXIDO NITRICO Limites: HUMANO Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/teses/BARRETO_JAISON/PDF/BARRETO_JAISON.pdf - pt. Localização: BR191.1; WC335.102.32, B275h

7 / 73

HANSEN

seleciona para imprimir Texto Completo-pt

Id: 23091 Autor: Belone, Andrea de Faria Fernandes Título: Hanseniase na faixa tuberculoide: estudo comparativo entre manifestacoes torpidas e reacionais atraves de avaliacoes de antigenos micabcterianos e de parametros representativos de hipersensibilidade medida por celulas, em biopsias cutaneas ?- Fonte: Botucatu; s.n; 2004. 94 p. ilus, tab. Tese: Apresentada a Universidade Estadual Paulista. Faculdade de Medicina de Botucatu para obtenção do grau de Doutor. Descritores: HANSENIASE TUBERCULOIDE/microbiol HANSENIASE TUBERCULOIDE/patol ANTIGENOS CITOCINAS CELULAS/imunol Limites: RELATO DE CASO Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/teses/BELONE_ANDREA/PDF/BELONE_ANDREA.pdf - pt. Localização: BR191.1; WC335.102.34, B418h

8 / 73

HANSEN

seleciona para imprimir

Id: 22742 Autor: Stefani, Mariane M. A; Martelli, Celina M. T; Gillis, Thomas P; Krahenbuhl, James L Título: In situ type 1 cytokine gene expression and mechanism associated with early leprosy progression ..- Fonte: s.l; s.n; 2003. 8 p. ilus. Resumo: We explored the prognostic value of in situ cytokine patterns in 39 patients with single-skin-lesion paucibacillary leprosy before single-dose therapy, with 3 years of follow-up. Interferon (IFN)-gamma, interleukin (IL)-12, IL-10, IL-4, tumor necrosis factor (TNF)-alpha, and macrophage inflammatory protein (MIP)-1alpha mRNA was quantified in skin biopsy samples at diagnosis, and Mycobacterium leprae DNA was detected in 51.4% of cases. Type 1 immunity predominance with measurable IFN-gamma and undetectable IL-4, which is indicative of effective cell-mediated immunity, is compatible with both the reversal reactions (33.3%) and the resolution of lesions (64.1%) observed. A positive correlation between IL-12 and IFN-gamma indicated type 1 polarization via IL-12. The TNF-alpha/MIP-1alpha correlation implied the TNF-alpha induction of chemokines, which is important for granuloma formation. Positive correlations between key regulatory cytokines-IL-10 and IFN-gamma, IL-10 and IL-12, and IL-10 and TNF-alpha-suggests that there may be some level of an intralesional pro- or anti-inflammatory mechanism essential in avoiding immunopathology. (AU). Descritores: Anticorpos Antibacterianos/BL Biópsia Estudos de Coortes Citocinas/BI/*GE/IM Regulação Bacteriana da Expressão Gênica/*IM Hanseníase/DT/*GE/IM Minociclina/TU Mycobacterium leprae/*GE/IM Ofloxacino/TU Prognóstico RNA Mensageiro/BI/GE RNA Viral/BI/GE Reação em Cadeia da Polimerase Via Transcriptase Reversa Rifampina/TU Limites: Adolescente Adulto Criança Feminino Humano Masculino Células Th1/IM Localização: BR191.1; 01884/s

9 / 73

HANSEN

seleciona para imprimir

Id: 22687 Autor: Moraes, M. O; Sarno, E. N; Almeida, A. S; Saraiva, B. C. C; Nery, J. A. C; Martins, R. C. L; Sampaio, E. P Título: Cytokine mRNA expression in leprosy; a possible role for interferon-gamma and interleukin-12 in reaction (RR and ENL) ..- Fonte: s.l; s.n; 1999. 9 p. tab, graf, ilus. Resumo: Leprosy patients during the natural course of the disease may develop reactional episodes, namely reversal reaction (RR) and erythema nodosum leprosum (ENL). Immunological events described as occurring during RR indicate up-regulation of the immune response, whereas in ENL the events are not fully understood. The aim of this study was to analyse the in vivo pattern of cytokine gene expression in the reactional states of leprosy. Peripheral blood mononuclear cells (PBMC, n = 14) and tissue samples (n = 17) obtained from patients with ENL and RR were obtained and assayed by RT-PCR. PBMC obtained from unreactional patients (n = 15) and normal individuals (n = 5) were also assessed. Expression of interferon (IFN)gamma, granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin (IL)-2Rp55, perforin and IL-1beta mRNA in PBMC were detected mostly in ENL/RR patients, but not in unreactional patients. Likewise, cytokines such as IL-6, IL-8, tumour necrosis factor (TNF)alpha and TNFbeta were also present in reactional and tuberculoid patients as opposed to lepromatous leprosy (BL/LL). Interestingly, the majority of ENL/RR patients showed messages for IL-6, IL-10, IL-12 and TNFalpha in the skin. IFNgamma was detected in 84.6% (ENL) and 100% (RR) of the patients, whereas IL-4 was detected only in few individuals (38.5 and 25%, respectively). Although mRNA expression and protein levels may be different, the data reported in this study suggest a cytokine mRNA profile that seems to be indistinguishable for RR and ENL. In addition, it shows up-regulation of immuno-inflammatory cytokines in the blood and tissue of the same patient examined before and during reaction. Furthermore, it is suggested that this pattern of response results from an immunological reactivation that might lead to an acute inflammatory response in both reactional episodes. (AU). Descritores: Sequência de Bases Estudos de Casos e Controles Citocinas/*GE Primers do DNA/GE Expressão Gênica Fator Estimulador de Colônias de Granulócitos-Macrófagos/GE Interferon Tipo II/*GE Interleucina-12/*GE Hanseníase/*GE/*IM Glicoproteínas de Membrana/GE Reação em Cadeia da Polimerase RNA Mensageiro/BL/*GE/*ME Limites: Humano Support, Non-U.S. Gov't Localização: BR191.1; 09183/s

10 / 73

HANSEN

seleciona para imprimir

Id: 22674 Autor: Bleharski, Joshua R; Li, Huiying; Meinken, Cristoph; Graeber, Thomas G; Ochoa, Maria-Teresa; Yamamura, Masahiro; Burdick, Anne; Sarno, Euzenir N; Wagner, Manfred; Röllinghoff, Martin; Rea, Thomas H; Colonna, Marco; Stenger, Steffen; Bloom, Barry R; Eisenberg, David; Modlin, Robert L Título: Use of genetic profiling in leprosy to discriminate clinical forms of the disease ..- Fonte: s.l; s.n; 2003. 4 p. . Resumo: Leprosy presents as a clinical and immunological spectrum of disease. With the use of gene expression profiling, we observed that a distinction in gene expression correlates with and accurately classifies the clinical form of the disease. Genes belonging to the leukocyte immunoglobulin-like receptor (LIR) family were significantly up-regulated in lesions of lepromatous patients suffering from the disseminated form of the infection. In functional studies, LIR-7 suppressed innate host defense mechanisms by shifting monocyte production from interleukin-12 toward interleukin-10 and by blocking antimicrobial activity triggered by Toll-like receptors. Gene expression profiles may be useful in defining clinical forms of disease and providing insights into the regulation of immune responses to pathogens. (AU). Descritores: Algoritmos Análise por Conglomerados Contagem de Colônia Microbiana Citocinas/GE/ME Perfilação da Expressão Gênica/* Regulação da Expressão Gênica/* Genes de Imunoglobulinas Imunidade Celular Imunidade Natural Hanseníase Virchowiana/*CL/*GE/IM/PP Hanseníase Tuberculóide/*CL/*GE/IM/PP Macrófagos Alveolares/MI Glicoproteínas de Membrana/IM Mycobacterium tuberculosis/GD/IM Análise de Sequência com Séries de Oligonucleotídeos Reação em Cadeia da Polimerase Análise de Componente Principal Receptores da Superfície Celular/IM Receptores Imunológicos/GE/ME Limites: Humano Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Regulação para Cima Localização: BR191.1; 09174/s

11 / 73

HANSEN

seleciona para imprimir

Id: 18986 Autor: Krutzik, Stephan R; Ochoa, Maria Teresa; Sieling, Peter A; Uematsu, Satoshi; Ng, Yolanda W; Legaspi, Annaliza; Liu, Philip T; Cole, Stewart T; Godowski, Paul J; Maeda, Yumi; Sarno, Euzenir N; Norgard, Michael V; Brennan, Patrick J; Akira, Shizuo; Rea, Thomas H; Modlin, Robert L Título: Activation and regulation of toll-like receptors 2 and 1 in human leprosy ..- Fonte: s.l; s.n; 2003. 8 p. ilus, graf. Resumo: The expression and activation of Toll-like receptors (TLRs) was investigated in leprosy, a spectral disease in which clinical manifestations correlate with the type of immune response mounted toward Mycobacterium leprae. TLR2-TLR1 heterodimers mediated cell activation by killed M. leprae, indicating the presence of triacylated lipoproteins. A genome-wide scan of M. leprae detected 31 putative lipoproteins. Synthetic lipopeptides representing the 19-kD and 33-kD lipoproteins activated both monocytes and dendritic cells. Activation was enhanced by type-1 cytokines and inhibited by type-2 cytokines. In addition, interferon (IFN)-gamma and granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced TLR1 expression in monocytes and dendritic cells, respectively, whereas IL-4 downregulated TLR2 expression. TLR2 and TLR1 were more strongly expressed in lesions from the localized tuberculoid form (T-lep) as compared with the disseminated lepromatous form (L-lep) of the disease. These data provide evidence that regulated expression and activation of TLRs at the site of disease contribute to the host defense against microbial pathogens. (AU). Descritores: CITOCINAS/fisiol HANSENIASE/imunol IMUNIDADE NATURAL LIPOPROTEINAS/anal GLICOPROTEINAS DE MEMBRANA/anal GLICOPROTEINAS DE MEMBRANA/fisiol RECEPTORES DA SUPERFICIE CELULAR/anal RECEPTORES DA SUPERFICIE CELULAR/fisiol Limites: HUMANO ANIMAL CAMUNDONGOS SUPPORT, NON-U.S. GOV'T SUPPORT, U.S. GOV'T, P.H.S. Meio Eletrônico: - . Localização: BR191.1; 09113/s

12 / 73

HANSEN

seleciona para imprimir

Id: 18946 Autor: Teles, Rosane M. B; Moraes, Milton O; Geraldo, Natasha T. R; Salles, Ana M; Sarno, Euzenir N; Sampaio, Elizabeth P Título: Differential TNFalpha mRNA regulation detected in the epidermis of leprosy patients ..- Fonte: s.l; s.n; 2002. 8 p. ilus, tab, graf. Resumo: The epidermis is an important site of the immunoinflammatory response in the skin. In the present study, the expression of cytokine and ICAM-1 (intercellular adhesion molecule-1) genes was evaluated by RT-PCR in the epidermis isolated from biopsies from 25 reactional leprosy patients. TNFalpha and IL-6 mRNAs were detected in all individuals during the reactional state (reversal reaction or erythema nodosum leprosum), IL-8 message was detected in 66.6 per cent and 62.5 per cent of the patients, IL-12 mRNA was present in 91.6 per cent and 62.5 per cent and ICAM-1 in 100 per cent and 71.4 per cent, respectively. In addition, when skin biopsies were obtained from the same patients before and during the reactional episode, an enhancement in cytokine mRNA, but not in ICAM-1 mRNA, was observed. Seven patients were also evaluated at the onset of reaction and during antiinflammatory treatment. In contrast to a preferential decrease in the TNFalpha gene detected in the dermis, during the treatment phase, persistent/enhanced TNFalpha mRNA expression was detected in the epidermis in six out of the seven patients assessed. This peculiar pattern of expression might reflect a differential impact that in vivo antiinflammatory therapy has on the epidermis. The present findings indicate that the epidermis plays an important role in the local inflammatory response in leprosy and that the profile of response detected in the epidermis during the reactions may be regulated differently from that in the dermis. (AU). Descritores: CITOCINAS/genet CITOCINAS/metab ANTIINFLAMATORIOS ESTERÓIDES/uso terap DERME/metab HANSENOSTATICOS/uso terap EPIDERME/metab MOLECULA 1 DE ADESAO INTERCELULAR/genet MOLECULA 1 DE ADESAO INTERCELULAR/metab HANSENIASE/quimioter HANSENIASE/metab PENTOXIFILINA/uso terap ANTIINFLAMATORIOS/uso terap RNA MENSAGEIRO/metab TALIDOMIDA/uso terap PREDNISONA/uso terap FATOR DE NECROSE TUMORAL/genet FATOR DE NECROSE TUMORAL/metab Limites: HUMANO MASCULINO FEMININO ADULTO MEIA-IDADE IDOSO SUPPORT, NON-U.S. GOV'T Meio Eletrônico: - . Localização: BR191.1; 09157/s

13 / 73

HANSEN

seleciona para imprimir

Id: 18575 Autor: Cheadle, Eleanor J; Selby, Peter J; Jackson, Andrew M Título: Mycobacterium bovis bacillus Calmette-Guérin-infected dendritic cells potently activate autologous T cells via a B7 and interleukin-12-dependent mechanism ..- Fonte: s.l; s.n; 2003. 10 p. graf. Resumo: Mycobacteria are potent adjuvants, can survive intracellularly and have been safely used for many years as vaccines against tuberculosis and leprosy. They are thus important potential vectors for recombinant vaccines. Many of their adjuvant properties are mediated following phagocytosis by dendritic cells (DC), which are in turn critical for priming naïve T cells. Although the maturation of DC in response to mycobacteria, such as Mycobacterium bovis bacillus Calmette-Guérin (BCG), is well described the subsequent responses of autologous T cells to mycobacterium-infected DC remains uncharacterized. In our experiments DC infected with BCG expressed more co-stimulatory molecules than tumour-necrosis factor-alpha (TNF-alpha) -treated DC and stimulated more potent mixed leucocyte reactions. When autologous T cells were co-cultured with BCG-exposed DC they became highly activated, as determined by display of CD25, CD54 and CD71 on both CD4+ and CD8+ cells. In contrast, the response of T cells to TNF-alpha-matured DC was significantly less. Cytokine production from T cells cultured with BCG-exposed DC was enhanced with elevated secretion of interleukin-2 (IL-2), IL-10 and interferon-gamma (IFN-gamma) and was produced by both CD4+ and CD8+ lymphocytes as determined by intracellular staining. In particular, IFN-gamma secretion was increased from 50 pg/ml to 25 000 pg/ml and IL-10 secretion increased from 20 pg/ml to 300 pg/ml in BCG-exposed DC co-cultures. Blocking antibodies to B7.1 and B7.2 or IL-12 significantly reduced the secretion of IFN-gamma and reductions were also seen in the expression of CD25 and CD71 by CD4+ cells. These data demonstrate that mycobacterially infected DC are particularly potent activators of autologous T cells compared to TNF-alpha-exposed DC and that the resultant T cells are functionally superior. (AU). Descritores: ANTIGENOS CD/metab ANTIGENOS CD80/imunol ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS B/metab LINFOCITOS T CD4-POSITIVOS/imunol LINFOCITOS T CD8-POSITIVOS/imunol CITOCINAS/bios CELULAS DENDRITICAS/imunol CELULAS DENDRITICAS/microbiol MOLECULA 1 DE ADESAO INTERCELULAR IMUNOFENOTIPAGEM INTERLEUCINA-12/imunol TRANSFORMACAO LINFOCITICA/imunol MYCOBACTERIUM BOVIS/imunol RECEPTORES DA INTERLEUCINA-2/metab LINFOCITOS T/imunol FATOR DE NECROSE TUMORAL/imunol REGULACAO PARA CIMA/imunol Limites: HUMANO SUPPORT, NON-U.S. GOV'T Meio Eletrônico: - . Localização: BR191.1; 08996/s

14 / 73

HANSEN

seleciona para imprimir

Id: 18504 Autor: Bleharski, Joshua R; Li, Huiying; Meinken, Christoph; Graeber, Thomas G; Ochoa, Maria-Teresa; Yamamura, Masahiro; Burdick, Anne; Sarno, Euzenir N; Wagner, Manfred; Röllinghoff, Martin; Rea, Thomas H; Colonna, Marco; Stenger, Steffen; Bloom, Barry R; Eisenberg, David; Modlin, Robert L Título: Use of genetic profiling in leprosy to discriminate clinical forms of the disease ..- Fonte: s.l; s.n; Sep. 2003. 4 p. graf. Resumo: Leprosy presents as a clinical and immunological spectrum of disease. With the use of gene expression profiling, we observed that a distinction in gene expression correlates with and accurately classifies the clinical form of the disease. Genes belonging to the leukocyte immunoglobulin-like receptor (LIR) family were significantly up-regulated in lesions of lepromatous patients suffering from the disseminated form of the infection. In functional studies, LIR-7 suppressed innate host defense mechanisms by shifting monocyte production from interleukin-12 toward interleukin-10 and by blocking antimicrobial activity triggered by Toll-like receptors. Gene expression profiles may be useful in defining clinical forms of disease and providing insights into the regulation of immune responses to pathogens. (AU). Descritores: HANSENIASE VIRCHOWIANA/clas HANSENIASE VIRCHOWIANA/genet HANSENIASE VIRCHOWIANA/imunol HANSENIASE VIRCHOWIANA/fisiopatol HANSENIASE TUBERCULOIDE/clas HANSENIASE TUBERCULOIDE/genet HANSENIASE TUBERCULOIDE/imunol HANSENIASE TUBERCULOIDE/fisiopatol CITOCINAS/genet CITOCINAS/metab PERFILACAO DA EXPRESSÃO GÊNICA ANALISE POR CONGLOMERADOS CONTAGEM DE COLÔNIA MICROBIANA REGULACAO DA EXPRESSÃO GÊNICA IMUNIDADE CELULAR IMUNIDADE NATURAL MACROFAGOS ALVEOLARES/microbiol GLICOPROTEINAS DE MEMBRANA/imunol MYCOBACTERIUM TUBERCULOSIS/cresc MYCOBACTERIUM TUBERCULOSIS/imunol REACAO EM CADEIA DA POLIMERASE RECEPTORES IMUNOLOGICOS/genet RECEPTORES IMUNOLOGICOS/metab RECEPTORES DA SUPERFICIE CELULAR/imunol - REGULACAO PARA CIMA  Análise de Componente Principal  ALGORITMOS  GENES DE IMUNOGLOBULINAS  ANALISE DE SEQUÊNCIA COM SERIES DE OLIGONUCLEOTIDIOS Limites: SUPPORT, NON-U.S. GOV'T HUMANO SUPPORT, U.S. GOV'T, P.H.S. Meio Eletrônico: - . Localização: BR191.1; 09142/s; BR191.1; 09145/s

15 / 73

HANSEN

seleciona para imprimir

Id: 16381 Autor: Adams, Linda B; Scollard, David M; Ray, Nashome A; Cooper, Andrea M; Frank, Anthony A; Orme, Ian M; Krahenbuhl, James L Título: The study of mycobacterium leprae infection in interferon-gamma gene - disrupted mice as a model to explore the immunopathologic spectrum of leprosy ..- Fonte: s.l; s.n; 2002. 8 p. ilus, graf. Descritores: LINFOCITOS T CD4-POSITIVOS LINFOCITOS T CD8-POSITIVOS CITOCINAS MODELOS ANIMAIS DE DOENÇAS CITOMETRIA DE FLUXO PELE PELE DELEÇAO DE GENES IMUNOHISTOQUIMICA INTERFERON TIPO II HANSENIASE HANSENIASE HANSENIASE TRANSFORMAÇAO LINFOCITICA MACROFAGOS PERITONEAIS CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS BALB C CAMUNDONGOS KNOCKOUT MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE Limites: ANIMAL Localização: BR191.1; 08655/s

16 / 73

HANSEN

seleciona para imprimir

Id: 16319 Autor: Sampaio, E. P; Hernandez, M. O; Carvalho, D. S; Sarno, E. N Título: Management of erythema nodosum leprosum by thalidomide: thalidomide analogues inhibit M. leprae-induced TNFalpha production in vitro ..- Fonte: s.l; s.n; 2002. 7 p. ilus, graf. Descritores: APOPTOSE CITOCINAS CITOCINAS ERITEMA NODOSO IMUNOSSUPRESSORES IMUNOSSUPRESSORES INTERLEUCINA-12 MONOCITOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE RNA MENSAGEIRO RNA MENSAGEIRO RNA MENSAGEIRO TALIDOMIDA TALIDOMIDA TALIDOMIDA FATOR DE NECROSE TUMORAL/AI/GE Localização: BR191.1; 08709/s

17 / 73

HANSEN

seleciona para imprimir

Id: 16185 Autor: Machado, Paulo Título: Produção de citocinas por células mononucleares do sangue periférico na hanseníase ?- Fonte: Salvador; s.n; 1997. vii,55 p. ilus, tab, graf. Tese: Apresentada a Universidade Federal da Bahia. Faculdade de Medicina para obtenção do grau de Mestre. Resumo: A hanseníase é uma doença espectral que apresenta grande variação no quadro clínico, diretamente relacionada com a resposta imune celular do hospedeiro dirigida contra os antígenos do Mycobacterium Leprae. Assim, no pólo tuberculóide, o quadro clínico de poucas lesões associa-se a uma baixa carga bacilar e à presença da resposta imune celular contra o bacilo de Hansen. No outro pólo da doença, a forma virchowiana, o quadro clínico de lesões disseminadas está associado a uma alta bacilífera e ausência de resposta imune celular. A produção de citocinas pelo sistema imune parece de fundamental importância na regulação da resposta imune humoral e celular, sendo por sua vez modulada por antígenos micobacterianos diversos. Portanto, as duas formas 'polares da hanseníase devem se diferenciar quanto ao perfil de produção de citocinas em resposta ao M. leprae, e difrentes antígenos micobacterianos devem modificar este perfil.(AU). Descritores: Citocinas Imunologia HANSENIASE Interferon Tipo II Localização: BR191.1; WC335.500, M119p

18 / 73

HANSEN

seleciona para imprimir

Id: 15813 Autor: Adams, Linda B; Scollard, David M; Ray, Nashone A; Cooper, Andrea M; Frank, Anthony A; Orne, Ian M; Krahenbuhl, James L Título: The study of Mycobacterium leprae infection in interferon-y gene-disrupted mice as a model to explore the immunopathologic spectrum of leprosy ..- Fonte: s.l; s.n; 2002. 8 p. ilus, graf. Descritores: LINFOCITOS T CD4-POSITIVOS LINFOCITOS T CD8-POSITIVOS CITOCINAS MODELOS ANIMAIS DE DOENÇAS CITOMETRIA DE FLUXO PÉ PÉ DELEÇAO DE GENES IMUNOHISTOQUIMICA INTERFERON TIPO II HANSENIASE HANSENIASE HANSENIASE TRANSFORMAÇAO LINFOCITICA MACROFAGOS PERITONEAIS CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS BALB C CAMUNDONGOS KNOCKOUT MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE ANIMAL LINFONODOS/IM Limites: ANIMAL Localização: BR191.1; 08511/s

19 / 73

HANSEN

seleciona para imprimir

Id: 15781 Autor: Moraes, M. O; Sampaio, E. P; Nery, J. A. C; Saraiva, B. C. C; Alvarenga, F. B. F; Sarna, E. N Título: Sequential erythema nodosum leprosum and reversal reaction with similar lesional cytokine mRNA pattern in a borderline leprosy patient ..- Fonte: s.l; s.n; 2001. 7 p. ilus, tab, graf. Descritores: HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA CITOCINAS CITOCINAS ERITEMA NODOSO ERITEMA NODOSO EXPRESSAO GENICA RNA MENSAGEIRO REAÇAO EM CADEIA POR POLIMERASE DNA POLIMERASE DIRIGIDO POR RNA FATOR DE NECROSE DE TUMOR Limites: RELATO DE CASO HUMANO MASCULINO Localização: BR191.1; 07353/s

20 / 73

HANSEN

seleciona para imprimir

Id: 15759 Autor: Moraes, Milton O; Sarno, Euzenir N; Teles, Rosane M. B; Almeida, Alexandre S; Saraiva, Bruno C. C; Nery, José A. C; Sampaio, Elizabeth P Título: Anti-inflammatory drugs block cytokine mRNA accumulation in the skin and improve the clinical condition of reactional leprosy patients ..- Fonte: s.l; s.n; 2000. 6 p. tab. Descritores: HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA ANTIINFLAMATORIOS ANTIINFLAMATORIOS BIOPSIA CITOCINAS CITOCINAS ERITEMA NODOSO ERITEMA NODOSO EXPRESSAO GENICA INTERFERON TIPO II INTERLEUCINA-4 REAÇAO EM CADEIA POR POLIMERASE PELE PELE FATOR DE NECROSE DE TUMOR Localização: BR191.1; 08424/s

---

página 1 de 4

ir para página

Refinar a pesquisa

Base de dados : HANSEN

Formulário avançado

Pesquisar por : Formulário livre

		Pesquisar	no campo
1			PalavrasPalavras do títuloPalavras do resumoAutorAutor institucionalDescritor de assuntoLimitesIdiomaPaís, ano de publicaçãoRevistaTipo de literaturaNível bibliográficoMonografia em sérieTipo de publicaçãoConferênciaIdentificador únicoLocalizaçãoSuporte eletrônicoData de entradaTipo de registro
2	and or and not		PalavrasPalavras do títuloPalavras do resumoAutorAutor institucionalDescritor de assuntoLimitesIdiomaPaís, ano de publicaçãoRevistaTipo de literaturaNível bibliográficoMonografia em sérieTipo de publicaçãoConferênciaIdentificador únicoLocalizaçãoSuporte eletrônicoData de entradaTipo de registro
3	and or and not		PalavrasPalavras do títuloPalavras do resumoAutorAutor institucionalDescritor de assuntoLimitesIdiomaPaís, ano de publicaçãoRevistaTipo de literaturaNível bibliográficoMonografia em sérieTipo de publicaçãoConferênciaIdentificador únicoLocalizaçãoSuporte eletrônicoData de entradaTipo de registro

Search engine: iAH v2.6.1 powered by WWWISIS

BIREME/PAHO/WHO - Latin American and Caribbean Center on Health Sciences Information