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| Id: | 19592
| | Author: | Nomaguchi, Hiroko; Matsuoka, Masanori; Kohsaka, Kenji; Nakata, Atsuo; Ito, Tonetaro. | | Title: | Overproduction, affinity purification and characterization of 65-kDA protein of mycobacterium leprae in Escharichia coli.
| | Source: | Int. J. Lep;57(4):817-824, dec. 1989. .
| | Abstract: | The 65-kDa protein of Mycobacterium leprae was produced in an Escherichia coli strain carrying a plasmid harboring the recloned gene coding for the protein. The protein was purified through affinity chromatography prepared with the IgG fraction of a monoclonal antibody which was prepared against the 65-kDa protein. The purified 65-kDa protein also reacted immunologically with the monoclonal antibody IIIE9, which recognizes the epitope for M. leprae, prepared by Buchanan, et al. BALB/c mice were inoculated with M. leprae and 4 months later were skin tested with the purified 65-kDa protein. Gross changes were observed at the skin-test site. The role of the protein in protective immunity against M. leprae foot pad infection in mice was also studied.
| | Descriptors: | Mycobacterium leprae/imunol
Mycobacterium leprae/fisiol
Escherichia coli/imunol
| | Electronic Medium: | http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n4/v57n4a10.pdf / en
| | Location: | Br191.1 |
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