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| Id: | 13450
| | Author: | Modlin, Robert L; Hofman, Florence M; Horwitz, David A; Husmann, Linda A; Gillis, Steven; Taylor, Clive R; Rea, Thomas H
| | Title: | In situ identification of cells in human leprosy granulomas with monoclonal antibodies to interleukin 2 and its receptor
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| | Source: | s.l; s.n; Jun. 1984. 6 p. ilus, tab, graf.
| | Abstract: | Leprosy is a chronic granulomatous disease with an immunologic spectrum in which lepromatous leprosy patients have defective cell-mediated immune responses, in comparison to tuberculoid leprosy patients. Immunoregulatory aspects of this spectrum were investigated by using monoclonal antibodies to interleukin 2 (IL 2), IL 2 receptors (Tac), and T lymphocyte subpopulations with immunoperoxidase techniques on frozen sections of skin biopsy specimens from 10 tuberculoid and 10 lepromatous patients. A comparison of IL 2+ cells revealed markedly fewer IL 2+ cells in lepromatous specimens (lep. 0.028% +/- 0.02 vs tub. 0.46% +/- 0.28, p less than 0.001). These IL 2+ cells were large, exhibited cytoplasmic staining, and on double immunostaining were Leu-4+, Leu-3a+, Leu-2a-, Tac-, and OKT6-, consistent with the fact they are IL 2 producers. Equivalent numbers of Tac+ cells were observed in both lepromatous and tuberculoid granulomas (lep. 1.5% +/- 0.5 vs tub. 2.1% +/- 0.7, p, NS), suggesting that the responder cells are present in both conditions. The tuberculoid granuloma was highly organized, composed of a central core of mature macrophages, Leu-3a+ and Tac+ cells with a surrounding mantle of Leu-2a+, Leu-3a+, IL 2+, Tac+, and OKT6+ cells. In lepromatous granulomas, Leu-2a+, Leu-3a+, Tac+, and rare IL 2+ cells were randomly admixed with bacilli-laden macrophages. The defective cell-mediated immune responses in lepromatous leprosy appears to be associated with diminished IL 2 production and disorganization of the granuloma.(AU).
| | Descriptors: | GRANULOMA/imunol
GRANULOMA/metab
HISTOCITOQUIMICA
INTERLEUCINA-2/bios
INTERLEUCINA-2/imunol
HANSENIASE/imunol
HANSENIASE/metab
LINFOCITOS T/clas
LINFOCITOS T/imunol
LINFOCITOS T/metab
| | Limits: | HUMANO
SUPPORT, NON-U.S. GOV'T
| | Electronic Medium: | http://www.ilsl.br
| | Location: | BR191.1; 01415/s |
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