Database : HANSEN Search on : METODOS [Subject descriptor] References found : 400 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19898 Author: Anon. Title: Monitoramento e avaliação do grupo de autocuidado / ? Source: In: Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica.Guia de apoio para grupos de autocuidado em hanseníase^ipt. Brasília, Editora do Ministério da Saúde, 2010. p.40-45tab. Descriptors: Autocuidado/instrum Autocuidado/métodos Location: BR191.1, B736g

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Id: 19896 Author: Anon. Title: Trabalhando com o grupo / ? Source: In: Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica.Guia de apoio para grupos de autocuidado em hanseníase^ipt. Brasília, Editora do Ministério da Saúde, 2010. p.16-31tab. Descriptors: Autocuidado/instrum Autocuidado/métodos Location: BR191.1, B736g

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Id: 19894 Author: Anon. Title: Grupos de apoio para o autocuidado / ? Source: In: Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica.Guia de apoio para grupos de autocuidado em hanseníase^ipt. Brasília, Editora do Ministério da Saúde, 2010. p.9-10. Descriptors: Autocuidado/métodos Autocuidado/util Location: BR191.1, B736g

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Id: 19893 Author: Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica*. Title: Guia de apoio para grupos de autocuidado em hanseníase^ipt ?- Source: Brasília; Editora do Ministério da Saúde; 2010. 47 p. tab. Descriptors: Hanseníase/prev Hanseníase/reabil Autocuidado/métodos Location: BR191.1; WC335.604, B736g

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Id: 19888 Author: Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica*. Title: Autocuidado em Hanseníase: face, mãos e pés^ipt ?- Source: Brasília; Editora do Minist´rio da Saúde; 2010. 71 p. ilus, tab. Descriptors: Hanseníase/prev Autocuidado/métodos Location: BR191.1; WC335.605, B736a

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Id: 19886 Author: São Paulo(Estado). Secretaria de Estado da Saúde. Coordenadoria de Controle de Doenças*. Title: Vigilância em Saúde: 20 anos SUS-SP^ipt ?- Source: São Paulo; s.n; 2008. 162 p. ilus, tab, graf. Descriptors: Sistema Único de Saúde/tend Saúde Pública/métodos Saúde Pública/tend Location: BR191.1; WA23, S63v

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Id: 19689 Author: Mostafa, Hamdy Mahfous; Kazda, Jindrich; Irgens, Lorents; Luesse, Hans Gerd. Title: Correspondence: Acid- Fast Bacilli from Former Leprosy Regions in Coastal Norway Showing PCR Positivity for Mycobacterium leprae. Source: Int. J. Lepr;63(1):97-99, 1995. ^bilus, ^btab. Descriptors: Mycobacterium leprae/fisiol Reação em Cadeia da Polimerase/métodos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1cor02.pdf / en Location: BR191.1

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Id: 19681 Author: Rafi, Abdolnasser; Donoghue, Helen D; Stanford, John L. Title: Application of Polymerase Chain Reaction for the Detection of Mycobacterium leprae DNA in Specimens from Treated Leprosy Patients. Source: Int. J. Lepr;63(1):42-47, 1995. ^btab. Abstract: Resumo: In this study of leprosy patients apparently cured by dapsone monotherapy, the polymerase chain reaction (PCR), one of the most reliable and sensitive DNA-based assays, was used for the specific detection of Mycobacterium leprae DNA. Sputum and slit-skin samples from 44 such patients at Baba Baghi Leprosy Sanatorium in Iran were examined. Primers for a 530-base-pair fragment of the gene encoding the 36-kDa antigen of M. leprae were used for the study. The PCR results were compared with microscopy for acid-fast bacilli. Of the 44 sputum samples, 2 were positive by PCR (4.5%) and of the 44 slit-skin swabs taken from the same patients, 10 were PCR positive (22.7%). Only one patient was PCR positive for both sputum and slit-skin specimens (2.3%). No positive results were found by acid-fast microscopy. In total, 11 of 44 (25%) patients in this study were found to be PCR positive for M. leprae, and it was thought probable that this indicated the presence of live organisms. Particularly interesting was the statistically significant association of positive results from slit-skin swabs with paucibacillary rather than multibacillary leprosy. It is suggested that whereas relapse or immunological reaction in paucibacillary disease may result from surviving organisms, in multibacillary leprosy this may be due to re-infection. Descriptors: Reação em Cadeia da Polimerase/métodos DNA/genet Hanseníase/genet Hanseníase/fisiopatol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1a07.pdf / en Location: BR191.1

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Id: 19680 Author: Misra, Namita; Ramesh, Venkatesh; Misra, Radhey S; Narayan, N. P. S; Colston, Michael Joseph; Nath, Indira. Title: Clinical Utility of LSR/A 15 Gene for Micobacterium leprae Detection in Leprosy Tissues Using the Polymerase Chain Reaction. Source: Int. J. Lepr;63(1):35-41, 1995. ^bilus, ^btab. Abstract: Resumo: Skin biopsy and slit-skin smears from 46 leprosy patients and 4 nonleprosy patients were tested for the presence of Mycobacterium leprae by the polymerase chain reaction (PCR) using primers based on the sequence of the LSR/15 kD gene. The PCR was found to be specific and sensitive, with a detection level of 10 and 100 bacilli. PCR using skin biopsies gave a higher detection rate than did slit-skin smears, probably due to the higher density of bacilli in a 4-mm punch biopsy. Dot blot hybridization with radioactive probes was 10-fold more sensitive than the ethidium bromide staining. Eight patients who did not show acid-fast bacilli in tissues by the conventional methods were shown to have PCR-amplified M. leprae DNA. False-negative results were obtained in 3 cases even though formal evidence for tissue inhibitors was absent. Descriptors: Mycobacterium leprae/genet Hanseníase/compl Hanseníase/diag Hanseníase/fisiopatol Reação em Cadeia da Polimerase/métodos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1a06.pdf / en

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Id: 19661 Author: Roche, Paul W; Failbus, Sarah S; Britton, Warwick J; Cole, Robert. Title: Rapid method for diagnosis of leprosy by measurements of antibodies to the M. leprae 35-kDa protein: comparison with PGL-1 antibodies detected by ELISA and "Dipstick" methods. Source: Int. J. Lepr;67(3):279-286, Sept., 1999. ilus, graf. Abstract: A new rapid immuno-chromatographic test card for the detection of antibodies to the Mycobacterium leprae 35-kD protein is described. The new assay is compared in the same group of subjects with a direct enzyme ELISA method for 35-kD antibodies and with assays for anti-phenolic glycolipid-I (PGL-I) antibodies using a standard ELISA as well as the recently described [quot ]dipstick[quot ] method. Good concordance was found between the rapid methods and the corresponding ELISA methods. The detection of untreated paucibacillary leprosy by the 35-kD test card was 59% compared with 27% for the PGL-I dipstick; however, the specificity for the 35-kD test card was 90% compared with 100% for the PGL-I dipstick in an endemic population. The potential application of these new, rapid serologic methods for the diagnosis of leprosy under field conditions is discussed. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/fisiol Anexina A3/imunol ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a07.pdf / en Location: BR191.1

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Id: 19595 Author: Matthews, CME. Title: Application of a health education model to obtain early and regular treatment of leprosy patients. Source: Int. J. Lep;57(4):844-846, dec. 1989. ^bgraf. Descriptors: Hanseníase/clas Hanseníase/epidemiol Educação em Saúde/métodos Educação em Saúde/util Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n4/v57n4clinot.pdf / en Location: Br191.1

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Id: 19580 Author: Gomes, Maria Katia; Stefani, Mariane; Sousa, AnaLúcia O. M; Rabello, Paula F. B; Pennini, Silmara; Narahashi, Kazuê; Ueda, Emília; Daxbacher, Egon R; Aslanian, Flávia M. N. P; Nery, José A. C; Sales, Anna Maria; Oliveira, Maria Leide W; Martelli, Celina Maria Turchi. Title: Single lesion leprosy pacients multicentric cohort treated with single dose drug therapy: findings on three-year follow-up and public health perspective in Brazil. Source: In: Universidade Federal do Rio de Janeiro.Instituto de Estudos em Saúde Coletiva.Investigações em sistema de saúde e controle da hanseníase^ipt. Rio de Janeiro, s.n, abr.-jun., 2008. p.363-376. Abstract: Single skin lesion, paucibacillary (SSL-PB) leprosy is considered and early disease manifesation. This study the clinical outcome of a cohort of 259 newly diagnosed SSL-PB treated with one dose of rifampicin, ofloxacin, minocycline (ROM) and followed-up for three-years. Patients were recruited from the North, Central West and Southeast regions in Brazil (1997-2001). The result expected with ROM therapy was disappearance or the reduction of lesion size. Manifestation that required additional intervention were considered as poor clinical outcome: type-1 reaction (T1R) with or without neuritis alone, increase in lesion size and shift from paucibacillary to multibacillary. The incidence of poor clinical outcome was calculated by person-month and with the Kaplan-Meier methods. 61.8% of the participants were females, mean age 32.2, and 67,2% had borderline tuberculoid (BT) or tuberculoid forms. T1R was the predominant event; shift from paucibacillary to multibacillaru was rare. 92.0% of the volunteers shown no events during the first year, the same occurring to 80.6% of them after 3 years of clinical monitoring. The probability of remaining event-free was highest among those 40 years old or younger. Poor outcome predominated among BT patients. Extended monitoring of SSL-PB leprosy cases under minimal therapy provided valuable case management information for reference centers. (AU)^ien. Descriptors: Hanseníase/epidemiol Hanseníase/imunol Hanseníase/fisiopatol Dose Única/métodos Saúde Pública/métodos Location: BR191.1; 01224/D.A

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Id: 19464 Author: Anon. Title: Molecular Biology. Source: Int. J. Lepr;69(2,suppl):S229-S230, Jun., 2001. . Conference: Present in: Proceedings Asian Leprosy Congress, Agra, 9-13 Nov., 2000. Descriptors: Hanseníase/fisiopatol Biologia Molecular/métodos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n2sup/v69n2supabs23.pdf / en Location: BR191.1

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Id: 19423 Author: Suneetha, Sujai; Reddy, Rajgopal. Title: Histological resolution and bacterial clearance with pulse ROM therapy in borderline lepromatous leprosy. Source: Int. J. Lepr;69(1):53-54, Mar., 2001. ilus. Descriptors: Hanseníase Virchowiana/imunol Hanseníase Virchowiana/fisiopatol Hanseníase Virchowiana/terap Pulsoterapia/métodos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1cor08.pdf / en Location: BR191.1

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Id: 19414 Author: Zumarraga, Martin Jose; Resoagli, Edmundo Hector; Civuta, Maria Helena; Martinez, Anibal Ramon; Rott, Maria Izabel Ortiz de; Millan, Sonnia Gracia de; Caimi, Karina; Gioffre, Andrea; Alito, Alicia; Bigi, Fabiana; Cataldi, Angel Adrian; Romano, Maria Isabel. Title: PCR-Restriction fragment length polymorphism analysis (PRA) of mycobacterium leprae from human lepromas and from a natural case of an armadillo of Corrientes, Argentina. Source: Int. J. Lepr;69(1):21-25, Mar., 2001. ilus. Abstract: Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) which relies on the amplification of a 439-bp portion of the hsp65 gene present in all mycobacteria, followed by two distinct digestions (with BstEII and HaeIII) of the PCR product, offers a rapid and easy alternative that allows identification of the species without the need for specialized equipment. Wild leprosy in the nine-banded armadillo (Dasypus novemcinctus) is characterized by the presence of multiple bacilli in internal organs such as lymph nodes, spleen and liver, as well as in nerves and skin. We could observe this in 9 out of 132 animals captured in Corrientes, Argentina, an area endemic for leprosy in humans. Mycobacterium leprae were recognized in those naturally infected animals through different techniques. Three samples of extracted DNA of the mycobacteria present in the spleen, liver and popliteal lymph node of a naturally infected animal during the Experimental Program in Armadillo (PEA) and three samples of human lepromas were processed by PRA. The patterns of the six samples analyzed were identical and were characteristic of M. leprae. These studies, made for the first time in Argentina, corroborate the initial discoveries in South America made by our investigative group on the detection of armadillos naturally infected with the Hansen bacillus.(AU)^ien. Descriptors: Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Limits: Animais Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1a03.pdf / en Location: BR191.1

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Id: 19412 Author: Truman, Richard W; Krahenbuhl, James L. Title: Viable M. leprae as a research reagent. Source: Int. J. Lepr;69(1):1-12, Mar., 2001. tab, graf. Abstract: Mycobacterium leprae remain a rare research resource. They cannot be cultivated on artificial media, and the only established means to quantify viability of M. leprae has been by its relative growth in the foot pads of conventional mice (MFP). The MFP method is technically difficult and requires several months to yield results. More effective methods are needed. We examined the association between M. leprae's ability to oxidize 14C-palmitate in axenic culture and the MFP growth results of a large number of suspensions. Oxidative activity was assessed by radiorespirometry (RR) using the Buddemeyer-type biphasic culture vessels containing 7H12 liquid medium and 14C-palmitate, or with commercially prepared BACTEC 12B vessels containing the same medium. The RR results were highly correlated (r = 0.71) with the growth level that each M. leprae suspension achieved by the MFP technique. In using this technique to examine the effects that many common laboratory practices have on M. leprae viability, we found that viability varies markedly between bacillary suspensions derived from different hosts and tissues. The highest viabilities were obtained with bacilli from moderately enlarged nude MFP (< 1 g). Viability tended to be lower among very large nude MFP or long-duration infections and from armadillo tissues. After their harvest from host tissues, leprosy bacilli lost viability quickly. Suspensions stored in 7H12 liquid medium retained < 1% of their viability within 3 weeks of harvest, and freezing bacillary preparations or incubating them at 37 degrees C resulted in nearly an immediate equivalent loss in metabolic activity and viability. M. leprae viability is maintained best when bacilli are stored for only short periods of time at 4 degrees C-33 degrees C. Palmitate oxidation is a rapid, reliable and objective means by which to estimate the viability of M. leprae and can be used effectively as a surrogate for the conventional MFP technique in many studies. (AU)^ien. Descriptors: Mycobacterium leprae/clas Pesquisa/instrum Pesquisa/métodos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1a01.pdf / en Location: BR191.1

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Id: 19380 Author: Shabaana, Abdul Khader; Venkatasubramani, Rajendran; Shanker Narayan, Nallakandypanangadan; Hoessli, Daniel C; Dharmalingam, Kuppamuthu. Title: Cytokine profiles in paraffin-embedded biopsy samples of lepromatous leprosy patients: semi-quantitative measure of cytokine mRNA using RT-PCR. Source: Int. J. Lepr;69(3):204-214, Sept., 2001. ilus, tab. Abstract: A reproducible technique for fixation of tissue, RNA extraction and reverse transcription polymerase chain reaction (RT-PCR) analysis from paraffin-embedded leprosy biopsies, has been developed and used to study the mRNA profiles. This approach is valuable in retrospective analysis of gene expression, and the handling of infectious biopsy material is also minimized. Among the methods of RNA extraction compared, the most efficient method was found to be incubation of the tissue sections in digestion buffer followed by extraction with Trizol. The experimental conditions were optimized for first strand cDNA synthesis and PCR, and used to measure the quantity of cytokine transcripts in biopsy materials. Interleukin-10 (IL-10) mRNA was detectable in all cases examined, which correlates well with other earlier reports using frozen tissues. However, IL-5 transcripts were present in 60% of the biopsies, unlike the earlier reports which showed IL-5 mRNA in all LL cases. Transforming growth factor-beta (TGF-beta) mRNA was detected in 80% of the biopsies, and this confirmed earlier immuno-cytochemical data which showed TGF-beta protein in all cases. Tumor necrosis factor-alpha mRNA was found in about 60% of the biopsies; whereas interferon gamma mRNA was detected in 30% of the LL cases. In conclusion, the results obtained in our study confirm and extend earlier observations which examined cytokines in peripheral blood cells and dermal lesions of leprosy. The simplicity of this method would allow the examination of a large number of samples across the spectrum of leprosy.(AU)^ien. Descriptors: Hanseníase Virchowiana/genet Hanseníase Virchowiana/imunol Hanseníase Virchowiana/fisiopatol Citocinas/genet RNA Mensageiro/genet Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n3/v69n3a04.pdf / en Location: BR191.1

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Id: 19362 Author: Gupta, U. D; Katoch, K; Sharma, R. K; Singh, H. B; Natragan, M; Singh, D; Sharma, V. D; Chauhan, D. S; Das, Ram; Srivastava, K; Katoch, V. M. Title: Analysis of quantitative relationship between viability determination in leprosy by MFP, ATP bioluminescence and gene amplification assay. Source: Int. J. Lepr;69(4):328-334, Dec., 2001. tab. Abstract: Two hundred twenty-one untreated, borderline lepromatous/lepromatous (BL/LL) leprosy patients have been investigated for viability by the mouse foot pad method (MFP), adenosine triphosphate (ATP) and polymerase chain reaction (PCR). The biopsies were collected at the beginning of and 12/24 months after treatment. The patient group was treated with a) immunotherapy (BCG/Mw) + MDT; b) MDT + pyrazinamide; c) control MDT; d) MDT + minocycline 100 mg once a month supervised + ofloxacin 400 mg once a month supervised. Biopsies were divided in three parts for use in the mouse foot pad, molecular and ATP investigations. In untreated and treated patients (at 12 and 24 months), there was a general agreement among all three techniques, and PCR and ATP showed higher positivity as compared to MFP. Further, there was good correlation among the viable biomass estimated by bacillary ATP levels, PCR assay and growth in mouse foot pads. The positivity was observed by MFP as well as PCR assay (18-kDa and 36-kDa) from all of the specimens when the ATP content was more than 3.6 pg/million. When the ATP content was below 3.5 pg/million, the positive takes in MFP decreased but the PCR positivity correlated with ATP bioluminescence up to 0.04 pg/million. When the ATP content was even lower, the uptake in the MFP was possibly a matter of chance, while PCR positivity was observed in 96% of the cases. For specimens with undetectable ATP, positivity was seen in 1% of the cases, showing the inability of ATP bioluminescence method to detect low background due to host ATP. PCR signals in some cases could be due to the higher sensitivity of the method or persistence of DNA after bacterial death in some cases. On the whole, the PCR methods even though targeting DNA have shown good correlations with biomass which confirm their usefulness in monitoring therapeutic responses in leprosy. (AU)^ien. Descriptors: Análise Quantitativa/métodos Trifosfato de Adenosina/uso diag Trifosfato de Adenosina/imunol Proteínas Luminescentes/uso diag Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n4/v69n4a04.pdf / en Location: BR191.1

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Id: 19359 Author: Jadhav, Rupendra S; Macdonald, Murdo; Bjune, G; Oskam, L. Title: Simplified PCR detection method for nasal Mycobacterium leprae. Source: Int. J. Lepr;69(4):299-307, Dec., 2001. ilus, tab. Abstract: We report here a simplified method for the detection of nasal carriage of Mycobacterium leprae. DNA extracted from nasal swabs was analyzed by PCR, and M. leprae specific amplicons detected by means of a novel peptide-nucleic-acid-ELISA (PNA-ELISA) method. Parameters for the method were established using swabs taken from untreated lepromatous leprosy patients. We have developed this method to study nasal carriage in endemic populations. However, due to the sensitivity of PCR based techniques, we wished to assess the possibility of false positive samples arising in our method. We therefore examined samples taken from individuals in Norway, a country non-endemic for leprosy, using our technique. A total of 219 nasal swabs were collected and tested in our laboratory in London. All of these were found to be negative by our criteria. In order to corroborate our results, and also to assess the specificity of the method, a small number of these samples were randomly selected, and a known amount of M. leprae DNA added to them. All 219 samples were then retested using the same techniques under [quot ]double blind[quot ] conditions in our laboratory in India. All of the samples to which M. leprae DNA had been added were successfully identified by this method whereas all other swabs were negative. Taken together, these results suggest that the technique described here is simple, sensitive, and specific for use in large-scale epidemiological studies. This study, part of the larger MILEP 2 study, represents the first use of a PNA-PCR method for an epidemiological study of infection. The method using PNA-ELISA is significantly simpler and more rapid than gel based detection methods. The supply of laboratory consumables and overall detection procedure were simplified and standardized by use of PCR Ready-to-Go beads.(AU)^ien. Descriptors: Reação em Cadeia da Polimerase/métodos Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n4/v69n4a01.pdf / en Location: BR191.1

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Id: 19245 Author: Wu, Qinxue; Yin, Yueping; Zhang, Liangfen; Chen, Xiaohong; Yu, Yanhau; Li, Zhicheng; Yu, Hua; Lu, Chengzhi; Feng, Suying; Li, Xiaojie; Huo, Wei; Ye, Ganyun. Title: A study on a possibility of predicting early relapse in leprosy using a ND-O-BSA based ELISA. Source: Int. J. Lepr;70(1):1-8, Mar.,2002. tab. Abstract: Serological methods have been used for detecting infection with Mycobacterium leprae. We have applied a serological test to explore the possibility it could detect a bacterial relapse among patients who have been cured with chemotherapy. More specifically we used an indirect enzyme-linked immunosorbant assay (ELISA) using the natural disaccharide (ND) of the phenolic glycolipid antigen of M. leprae linked to bovine serum albumin as antigen. Antibody levels were measured in sera from normal controls, active leprosy cases, cured leprosy patients, and relapsing leprosy patients. We correlated antibody levels with the type of leprosy, the bacterial index, and with relapse among cured leprosy patients. In our hands, the ND-ELISA, when applied to screening for infection with M. leprae, had excellent sensitivity, specificity, positive and negative predictive values, and both a low false positive rate and a low false negative rate. Antibody levels gradually increased among active patients from the tuberculoid to the lepromatous end of the leprosy spectrum. There was a year-by-year fall in antibody levels in patients responding to chemotherapy. Antibody levels and the bacterial index were correlated using the Spearman's rank correlation method. Serial antibody levels were measured in 666 leprosy patients after being cured with dapsone monotherapy. Over a three year follow up, 95 multibacillary patients became antibody positive and 12 of them had bacterial relapses of their disease. In contrast, among 335 cases that remained antibody negative, only one relapse was seen. Among 44 paucibacillary cured patients who became antibody positive, there was one relapse. There were 192 such patients who remained antibody negative and one relapsed. The risk of relapse is 6.7 times higher among cured multibacillary patients compared to cured paucibacillary patients. Overall, the cumulative relapse rate among antibody positive cases was 13.7%, compared to 0.4% among antibody negative patients. We conclude that the ND-ELISA is a useful tool both for screening for early infection with M. leprae and for predicting a relapse in cured patients, particularly in cured multibacillary patients. (AU)^ien. Descriptors: Hanseníase/imunol Hanseníase/fisiopatol ELISA/instrum ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n1/v70n1a01.pdf / en Location: BR191.1

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