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Id: 27279 Autor: Barrera, Silvia de la; Finiasz, Marta; Fink, Susana; Valdez, Raul; Bottasso, Oscar; Balina, Luis Maria; Sasiain, Maria del Carmen. Título: Differential development of CD4 and CD8 cytotoxic T cells (CTL) in PBMC across the leprosy spectrum IL-6 with IFN-gamma or IL-2 generate CTL in multibacillary patients. Fonte: Int. J. Lepr;65(1):45-55, Mar., 1997. tab, graf. Resumo: In the present study we evaluated the contribution of CD4 and CD8 T cells on the antigen-specific cytotoxic activity induced by whole Mycobacterium leprae in leprosy patients and normal controls (N) as well as the modulation of this activity by some cytokines. Peripheral blood mononuclear cells (PBMC) from N or from leprosy patients were stimulated with antigen in the presence or absence of cytokines for 7 days. M. leprae-stimulated PBMC were depleted of CD4 or CD8 antigen-bearing cells and employed as effector cells in a 4-hr [31Cr]-release assay against autologous M. leprae-pulsed macrophages. Our results demonstrate that both CD4 and CD8 T cells contribute to M. leprae-induced cytotoxic activity, with differences observed in paucibacillary (PB) and multibacillary (MB) patients. CD8-mediated cytotoxic activity is higher than that of CD4 cells in PB patients, while in MB patients CD4 cytotoxicity is predominant. Our data also demonstrate that the generation of CD4 and CD8 cytotoxic T lymphocytes (CTL) can be modulated differentially by interleukin-4 (IL-4), IL-6, gamma interferon (IFN-gamma), or IL-2. Although MB patients developed the lowest CTL response, cytokines such as IL-6 plus IL-2 or IFN-gamma were able to generate both CD4 and CD8 cytotoxic T cells from MB patients. In PB patients, IL-6 plus IFN-gamma displayed the highest stimulation on CD8 effector cells. Thus, an important role may be assigned to IL-6, together with IL-2 or IFN-gamma, in the differentiation of M. leprae-specific CTL effector cells. (AU)^ien. Descritores: Linfócitos T CD4-Positivos/imunol Linfócitos T CD8-Positivos/imunol Hanseníase Dimorfa/imunol Hanseníase Virchowiana/imunol Hanseníase Tuberculóide/imunol Localização: BR191.1

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Id: 27150 Autor: Ramos, Teresa; Zalcberg-Quintana, Ilana; Appelberg, Rui; Sarno, Euzenir N; Silva, Manuel T. Título: T-helper cell subpopulations and the immune spectrum of leprosy. Fonte: Int J Lep;57(1):73-81, Mar. 1989. . Descritores: Hanseníase/genet Hanseníase/imunol Linfócitos T Auxiliares-Indutores/clas Linfócitos T Auxiliares-Indutores/imunol Limites: Humanos Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n1/v57n1edt01.pdf - en. Localização: Br191.1

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Id: 27141 Autor: Mustafa, Abu Salim; Qvigstad, Erik. Título: HLA-DR-restricted antigen induced proliferation and cytotoxicity mediated by CD4+ T-cell clones from subjects vaccinated with killed M. leprae. Fonte: Int J Lep;57(1):1-11, Mar. 1989. ^btab. Resumo: Thirteen CD4+ T-cell clones raised against Mycobacterium leprae from three M. leprae-vaccinated subjects were studied for major histocompatibility complex (MHC) restriction in proliferative and cytotoxicity assays. These T-cell clones recognized at least nine different epitopes, ranging from M. leprae-specific to broadly crossreactive. Restriction studies with a panel of antigen-presenting cells (APCs) suggest that all of the T-cell clones recognized antigens in the context of the DR locus. Three T-cell clones with three different reactivities from a DR1, 2-positive subject responded to M. leprae in proliferation and cytotoxicity when the antigen was presented in the context of DR1-positive APCs. Four T-cell clones responding to M. leprae-specific or crossreactive epitopes from the second donor, who was DR4,DW4; DR4,Dw14-positive, and a single M. leprae-specific T-cell clone from the third subject, who was DR3,4:Dw4, recognized the antigens in the presence of Dw4 APCs. Four crossreactive T-cell clones from the second subject responded in the presence of Dw14-positive APCs, and one limited crossreactive clone recognized the antigen in the context of DR4 and DR7-positive cells, suggesting that its response was restricted by a common determinant. The T-cell clones that recognize the 65-kDa, 18-kDa, and 13B3 recombinant M. leprae antigens in proliferative assays were cytotoxic for autologous adherent cells pulsed with the respective antigens^ien. Descritores: Hanseníase/imunol Mycobacterium leprae/imunol Linfócitos/imunol Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n1/v57n1a01.pdf - en. Localização: Br191.1

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Id: 26662 Autor: Huizar-López, Rosario; Santerre, Ann; Madrid-Marina, Vicente; Peralta-Zaragoza, Oscar; Villalobos-Arámbula, Alma; Islas-Rodríguez, Alfonso. Título: AP-1 Is present in nuclear extracts of lymphocytes from lepromatous leprosy patients. Fonte: Int. J. Lepr;70(4):274-277, Dec., 2002. ilus, tab. Descritores: Hanseníase Virchowiana/imunol Hanseníase Virchowiana/fisiopatol Antígenos CD95/genet Antígenos CD95/imunol Linfócitos/imunol Linfócitos/fisiol Limites: Humanos Meio Eletrônico: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n4/v70n4cor.pdf - en. Localização: BR191.1

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Id: 25255 Autor: Reinherz, Ellis L; Schlossman, Stuart F Título: The differentiation and function of human T lymphocytes ..- Fonte: s.l; s.n; 1980. 7 p. tab, graf. Descritores: ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS B/anal ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS B/sangue ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS B/imunol ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS T/anal ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS T/sangue ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS T/imunol ANTIGENOS SECUNDARIOS DE ESTIMULACAO DE LINFOCITOS/anal ANTIGENOS SECUNDARIOS DE ESTIMULACAO DE LINFOCITOS/sangue ANTIGENOS SECUNDARIOS DE ESTIMULACAO DE LINFOCITOS/imunol LINFOCITOS/sangue LINFOCITOS/clas LINFOCITOS/imunol - INTERLEUCINA-1/anal  INTERLEUCINA-1/sangue  INTERLEUCINA-1/imunol  ANTICORPOS MONOCLONAIS/bios  ANTICORPOS MONOCLONAIS/isol  ANTICORPOS MONOCLONAIS/farmacocin  IMUNOFLUORESCÊNCIA/util Limites: HUMANO ANIMAL Localização: BR191.1; 00799/s

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Id: 25126 Autor: Quiroga, Maria F; Martinez, Gustavo J; Pasquinelli, Virginia; Costas, Monica A; Bracco, Maria M; Malbran, Aleajndro; Olivares, Liliana M; Sieling, Peter A; Garcia, Veronica E Título: Activation of signaling lymphocytic activation molecule triggers a signaling cascade that enhances Th1 responses in human intracellular infection ..- Fonte: s.l; s.n; 2004. 10 p. graf. Resumo: T cell production of IFN-gamma contributes to host defense against infections by intracellular pathogens, including mycobacteria. Lepromatous leprosy, the dissminated from of infection caused by Mycobacterium leprae, is characterized by loss of cellular response against the pathogen and diminished Th1 cytokine production. Relieving bacterial burden in Ag-unresponsive patients might be achieved through alternative receptors that stimulate IFN-gamma production. We have previously shown that ligation of signaling lymphocytic activation molecule (SLAM) enhances IFN-gamma in mycobacterial infection; therefore, we investigated molecular pathways leading from SLAM activation to IFN-gamma production in human leprosy. The expression of the SLAM-associated protein (an inhibitory factor for IFN-gamma induction) on M. leprae-stimulated cells from leprosy patients was inversely correlated to IFN-gamma production. Howevwe, SLAM ligation or exposure of cell from lepromatous patients to a proinflammatory microenvironment down-regulated SLAM-associated protein expression. Moreover. SALAM activation induced a sequence of signaling proteins, including activation of the NF-kB complex, phosphorylation of Stat1, and induction of T-bet expression, resulting in the promotion a cascade of molecular events during signaling through SLAM in leprosy that cooperate to induce INF-gamma production and strongly suggest that SLAM might be a focal point for therapeutic modulation of the cell cytokine responses in diseases characterized by dysfunctional Th2 responses (AU). Descritores: HANSENIASE/imunol HANSENIASE/microbiol LINFOCITOS T/imunol LINFOCITOS T/microbiol INTERLEUCINAS/sangue INTERLEUCINAS/imunol - LINFOCITOS/imunol  LINFOCITOS/microbiol  MYCOBACTERIUM LEPRAE/imunol  MYCOBACTERIUM LEPRAE/patogen Limites: HUMANO Localização: BR191.1; 09192/s

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Id: 24504 Autor: Brennan, Patrick J. Título: Mycobacterium leprae: the significance of our knowledge of its composition and antigenicity. Fonte: Hansen. int;(n.esp):103-110, Jun. 1998. . Conferência: Apresentado em: Congresso da Associação Brasileira de Hansenologia, IX, Foz do Iguaçu, 04-08 junho 1997. Descritores: MYCOBACTERIUM LEPRAE/cresc MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/fisiol LINFOCITOS T CD4-POSITIVOS/citol LINFOCITOS T CD4-POSITIVOS/fisiol Limites: ESTUDO COMPARATIVO HUMANO Localização: BR191.1

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Id: 23970 Autor: Geluk, Annemieke; Ottenhoff, Tom H. M Título: HLA and leprosy in the pre and postgenomic eras ..- Fonte: s.l; s.n; 2006. 7 p. graf. Resumo: Leprosy has intrigued immunologists for many decades. Despite minimal genetic variation between Mycobacterium leprae isolates worldwide, two completely different forms of the disease can develop in the susceptible human host: localized, tuberculoid, or paucibacillary leprosy, which can heal spontaneously, and disseminating, lepromatous, or multibacillary leprosy, which is progressive if untreated. The questions which host factors regulate these very different outcomes of infection, by what mechanisms, and whether these can be used to combat disease remain unanswered. Leprosy has been one of the very first human diseases in which human leukocyte antigen (HLA) genes were demonstrated to codetermine disease outcome. Jon van Rood was among the earliest researchers to recognize the potential of this ancient disease as a human model to dissect the role of HLA in disease. Decades later, it is now clear that HLA molecules display highly allele-specific peptide binding capacity. This restricts antigen presentation to M. leprae-reactive T cells and controls the magnitude of the ensuing immune response. Furthermore, specific peptide/HLA class II complexes can also determine the quality of the immune response by selectively activating regulatory (suppressor) T cells. All these factors are believed to contribute to leprosy disease susceptibility. Despite the global reduction in leprosy disease prevalence, new case detection rates remain invariably high, demonstrating that treatment alone does not block transmission of leprosy. Better tools for early detection of preclinical M. leprae infection, likely the major source of unidentified transmission, therefore is a priority. Newly developed HLA-based bioinformatic tools now provide novel opportunities to help combat this disease. Here, we describe recent work using HLA-DR peptide binding algorithms in combination with recently elucidated genome sequences of several different mycobacteria. Using this postgenomic HLA-based approach, we were able to identify 12 candidate genes that were unique to M. leprae and were predicted to contain T cell epitopes restricted via several major HLA-DR alleles. Five of these antigens (ML0576, ML1989, ML1990, ML2283, ML2567) were indeed able to induce significant T cell responses in paucibacillary leprosy patients and M. leprae-exposed healthy controls but not in most multibacillary leprosy patients, tuberculosis patients, or endemic controls...(AU). Descritores: Motivos de Aminoácidos Anticorpos Antibacterianos/BL Antígenos de Bactérias/IM Sítios de Ligação Epitopos de Linfócito T Genes Classe II do Complexo de Histocompatibilidade (MHC) Genoma Bacteriano Glicolipídeos/IM Antígenos HLA-DR/*GE/IM Hanseníase/DI/*IM/MI Hanseníase Virchowiana/DI/IM/MI Hanseníase Tuberculóide/DI/IM/MI Mycobacterium leprae/GE/*IM Linfócitos T/IM/MI Limites: HUMANO Research Support, Non-U.S. Gov´t Localização: BR191.1; 09362/S

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Id: 23196 Autor: Geluk, A; van Meijgaarden, K. E; Franken, K. L. M. C; Wieles, B; Arend, S. M; Faber, W. R; Naafs, B; Ottenhoff, T. H. M Título: Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis ..- Fonte: s.l; s.n; 2004. 5 p. tab, graf. Resumo: Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all tested M. bovis bacille Calmette-Guérin (BCG) strains but present in M. leprae, M. tuberculosis, M. bovis, M. kansasii, M. africanum and M. marinum. In this study, the homologue of CFP-10 in M. leprae (ML0050) is identified and characterized. Interferon-gamma production in response to this homologue by T cells from leprosy patients, TB patients and unexposed controls shows that CFP-10 of M. leprae is a potent antigen that crossreacts with CFP-10 of M. tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases. (AU). Descritores: Sequência de Aminoácidos Antígenos de Bactérias/IM Proteínas de Bactérias/*IM Reações Cruzadas/IM Interferon Tipo II/IM/SE Hanseníase/*IM Ativação Linfocítica/IM Dados de Sequência Molecular Mycobacterium leprae/*IM Mycobacterium tuberculosis/*IM Homologia de Sequência Linfócitos T/IM/ME Tuberculose/*IM Limites: Research Support, Non-U.S. Gov't Humanos Estudo Comparativo Animais Localização: BR191.1; 09340/s

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Id: 23106 Autor: Kennedy, Cornelis; Lien, Roel A. M. Chin; Stolz, Ernst; Joost, Theodoor; Naafs, Bernard Título: Leprosy and human immunodeficiency virus infection: a closer look at the lesions ..- Fonte: s.l; s.n; 1990. 2 p. . Descritores: Síndrome de Imunodeficiência Adquirida/*CO/PA Infecções por HIV/*CO/PA Ceratinócitos/PA Células de Langerhans/PA Hanseníase Dimorfa/CO/*PA Hanseníase Virchowiana/CO/*PA Linfócitos T Citotóxicos/PA Linfócitos T Auxiliadores-Indutores/PA Linfócitos T Supressores-Efetores/PA Limites: FEMININO ADULTO Humanos Localização: BR191.1; 09234/s

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Id: 22741 Autor: Black, Gillian F; Weir, Rosemari E; Chaguluka, Steven D; Warndorff, David; Crampin, Amelia C; Mwaungulu, Lorren; Sichali, Lifted; Floyd, Sian; Bliss, Lyn; Jarman, Elizabeth; Docovan, Linda; Andersen, Peter; Britton, Warwick; Hewinson, Glyn; Huygen, Kris; Paulsen, Jean; Sing, Mahavir; Prestidge, Ross; Fine, Paul E. M; Dockrell, Hazel M Título: Gama interferon responses induced by a panel of recombinant and purified mycobacterial antigens in healthy, non-Mycobacterium bovis BCG-vaccinated Malawian young adults ..- Fonte: s.l; s.n; 2003. 10 p. graf. Resumo: We have previously shown that young adults living in a rural area of northern Malawi showed greater gamma interferon (IFN-gamma) responses to purified protein derivatives (PPD) prepared from environmental mycobacteria than to PPD from Mycobacterium tuberculosis. In order to define the mycobacterial species to which individuals living in a rural African population have been exposed and sensitized, we tested T-cell recognition of recombinant and purified antigens from M. tuberculosis (38 kDa, MPT64, and ESAT-6), M. bovis (MPB70), M. bovis BCG (Ag85), and M. leprae (65 kDa, 35 kDa, and 18 kDa) in >600 non-M. bovis BCG-vaccinated young adults in the Karonga District of northern Malawi. IFN-gamma was measured by enzyme-linked immunosorbent assay (ELISA) in day 6 supernatants of diluted whole-blood cultures. The recombinant M. leprae 35-kDa and 18-kDa and purified native M. bovis BCG Ag85 antigens induced the highest percentages of responders, though both leprosy and bovine tuberculosis are now rare in this population. The M. tuberculosis antigens ESAT-6 and MPT64 and the M. bovis antigen MPB70 induced the lowest percentages of responders. One of the subjects subsequently developed extrapulmonary tuberculosis; this individual had a 15-mm-diameter reaction to the Mantoux test and responded to M. tuberculosis PPD, Ag85, MPT64, and ESAT-6 but not to any of the leprosy antigens. We conclude that in this rural African population, exposure to M. tuberculosis or M. bovis is much less frequent than exposure to environmental mycobacteria such as M. avium, which have antigens homologous to the M. leprae 35-kDa and 18-kDa antigens. M. tuberculosis ESAT-6 showed the strongest association with the size of the Mantoux skin test induration, suggesting that among the three M. tuberculosis antigens tested it provided the best indication of exposure to, or infection with, M. tuberculosis. (AU). Descritores: Antígenos de Bactérias/*IM Vacina BCG/*IM Interferon Tipo II/*BI Hanseníase/EP Malauí/EP Mycobacterium/GE/*IM Mycobacterium bovis/IM Mycobacterium leprae/IM Mycobacterium tuberculosis/IM Proteínas Recombinantes de Fusão/IM Especificidade de Espécies Linfócitos T/IM Teste Tuberculínico Tuberculose/IM Vacinação Limites: Adolescente Adulto Criança Estudo Comparativo Feminino Humano Masculino População Rural Localização: BR191.1; 00224/s

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Id: 22738 Autor: Kirkaldy, A. A; Musonda, A. C; Khanolkhar-Young, S; Suneetha, S; Lockwood, D. N. J Título: Expression of CC and CXC chemokines and chemokine receptors in human in human leprosy skin lesions ..- Fonte: s.l; s.n; 2003. 7 p. ilus, tab, graf. Resumo: We have investigated the expression of chemokines and their receptors in leprosy skin lesions using immunohistochemistry. Skin biopsies from 25 leprosy patients across the leprosy spectrum, 11 patients undergoing type I reversal reactions and four normal donors were immunostained by ABC peroxidase method using antibodies against CC and CXC chemokines and their receptors. Using an in situ hybridization technique we have also studied the expression of monocyte chemoattractant protein 1 (MCP-1), RANTES and interleukin (IL)-8 chemokines mRNA in leprosy skin lesions. Chemokines and receptor expression was detected in all leprosy skin biopsies. Expression of CC chemokines MCP-1 (P < 0.01) and RANTES (P < 0.01) were elevated significantly in borderline tuberculoid leprosy in reversal reaction compared to non-reactional borderline tuberculoid leprosy, but there was no difference in the expression of IL-8 chemokine. Surprisingly, there was no significant difference in the expression of CC (CCR2 and CCR5) and CXC (CXCR2) chemokine receptors across the leprosy spectrum. Similarly, there was no significant difference in the expression of mRNA for MCP-1, regulated upon activation normal T cell expressed and secreted (RANTES) and IL-8 chemokines. Here, the presence of a neutrophil chemoattractant IL-8 in leprosy lesions, which do not contain neutrophils, suggests strongly a role of IL-8 as a monocyte and lymphocyte recruiter in leprosy lesions. These results suggest that the chemokines and their receptors, which are known to chemoattract T lymphocytes and macrophages, are involved in assembling the cellular infiltrate found in lesions across the leprosy spectrum. (AU). Descritores: Linfócitos T CD4-Positivos/IM Linfócitos T CD8-Positivos/IM Quimiocinas/*AN/GE Imunohistoquímica/MT Hibridização In Situ/MT Interleucina-8/GE Hanseníase/*IM Macrófagos/IM Proteína-1 Quimioatraente de Monócito/GE RANTES/GE RNA Mensageiro/AN Receptores CCR5/AN Receptores de Quimiocinas/*AN/GE Receptores de Interleucina-8B/AN Limites: Humano Pele/*IM Estatísticas não Paramétricas Localização: BR191.1; 00253/s

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Id: 18951 Autor: Maeda, Yumi; Gidoh, Masaichi; Ishii, Norihisa; Mukai, Chifumi; Makino, Masahiko Título: Assessment of cell mediated immunogenicity of Mycobacterium leprae-derived antigens ..- Fonte: s.l; s.n; 2003. 9 p. tab, graf. Resumo: The antigenicity of Mycobacterium leprae (M. leprae)-derived cell membrane fraction was examined using human dendritic cells (DCs). Immature DCs internalized and processed the cell membrane components, and expressed M. leprae-derived antigens (Ags) on their surface. The expression of MHC class II, CD86, and CD83 Ags on DCs and CD40 ligand (L)-associated IL-12 p70 production from DCs were up-regulated by the membrane Ags. Moreover these stimulated DCs induced significantly higher level of interferon-gamma (IFN-gamma) production by autologous CD4(+) and CD8(+) T cells than those pulsed with equivalent doses of live M. leprae or its cytosol fraction. Both subsets of T cells from tuberculoid leprosy patients also produced several fold more IFN-gamma than those from normal individuals. Furthermore, the intracellular perforin production in CD8(+) T cells was up-regulated in an Ag-dose dependent manner. These results suggest that M. leprae membrane Ags might be useful as the vaccinating agents against leprosy. (AU). Descritores: ANTIGENOS DE BACTERIAS/imunol ANTIGENOS DE SUPERFÍCIE/imunol LINFOCITOS T CD4-POSITIVOS/imunol LIGANTE A CD40/fisiol LINFOCITOS T CD8-POSITIVOS/imunol CELULAS DENDRITICAS/imunol INTERFERON TIPO II/bios INTERLEUCINA-12/bios TRANSFORMACAO LINFOCITICA GLICOPROTEINAS DE MEMBRANA/bios MYCOBACTERIUM LEPRAE/imunol Limites: HUMANO SUPPORT, NON-U.S. GOV'T SUPPORT, U.S. GOV'T, P.H.S. Meio Eletrônico: - . Localização: BR191.1; 09083/s

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Id: 18575 Autor: Cheadle, Eleanor J; Selby, Peter J; Jackson, Andrew M Título: Mycobacterium bovis bacillus Calmette-Guérin-infected dendritic cells potently activate autologous T cells via a B7 and interleukin-12-dependent mechanism ..- Fonte: s.l; s.n; 2003. 10 p. graf. Resumo: Mycobacteria are potent adjuvants, can survive intracellularly and have been safely used for many years as vaccines against tuberculosis and leprosy. They are thus important potential vectors for recombinant vaccines. Many of their adjuvant properties are mediated following phagocytosis by dendritic cells (DC), which are in turn critical for priming naïve T cells. Although the maturation of DC in response to mycobacteria, such as Mycobacterium bovis bacillus Calmette-Guérin (BCG), is well described the subsequent responses of autologous T cells to mycobacterium-infected DC remains uncharacterized. In our experiments DC infected with BCG expressed more co-stimulatory molecules than tumour-necrosis factor-alpha (TNF-alpha) -treated DC and stimulated more potent mixed leucocyte reactions. When autologous T cells were co-cultured with BCG-exposed DC they became highly activated, as determined by display of CD25, CD54 and CD71 on both CD4+ and CD8+ cells. In contrast, the response of T cells to TNF-alpha-matured DC was significantly less. Cytokine production from T cells cultured with BCG-exposed DC was enhanced with elevated secretion of interleukin-2 (IL-2), IL-10 and interferon-gamma (IFN-gamma) and was produced by both CD4+ and CD8+ lymphocytes as determined by intracellular staining. In particular, IFN-gamma secretion was increased from 50 pg/ml to 25 000 pg/ml and IL-10 secretion increased from 20 pg/ml to 300 pg/ml in BCG-exposed DC co-cultures. Blocking antibodies to B7.1 and B7.2 or IL-12 significantly reduced the secretion of IFN-gamma and reductions were also seen in the expression of CD25 and CD71 by CD4+ cells. These data demonstrate that mycobacterially infected DC are particularly potent activators of autologous T cells compared to TNF-alpha-exposed DC and that the resultant T cells are functionally superior. (AU). Descritores: ANTIGENOS CD/metab ANTIGENOS CD80/imunol ANTIGENOS DE DIFERENCIACAO DE LINFOCITOS B/metab LINFOCITOS T CD4-POSITIVOS/imunol LINFOCITOS T CD8-POSITIVOS/imunol CITOCINAS/bios CELULAS DENDRITICAS/imunol CELULAS DENDRITICAS/microbiol MOLECULA 1 DE ADESAO INTERCELULAR IMUNOFENOTIPAGEM INTERLEUCINA-12/imunol TRANSFORMACAO LINFOCITICA/imunol MYCOBACTERIUM BOVIS/imunol RECEPTORES DA INTERLEUCINA-2/metab LINFOCITOS T/imunol FATOR DE NECROSE TUMORAL/imunol REGULACAO PARA CIMA/imunol Limites: HUMANO SUPPORT, NON-U.S. GOV'T Meio Eletrônico: - . Localização: BR191.1; 08996/s

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Id: 18557 Autor: Cardoso, Fernando L. L; Antas, Paulo R. Z; Milagres, Alexandre S; Geluk, Annemieke; Franken, Kees L. M. C; Oliveira, Eliane B; Teixeira, Henrique C; Nogueira, Susie A; Sarno, Euzenir N; Klatser, Paul; Ottenhoff, Tom H. M; Sampaio, Elizabeth P Título: T-cell responses to the Mycobacterium tuberculosis-specific antigen ESAT-6 in Brazilian tuberculosis patients ..- Fonte: s.l; s.n; 2002. 8 p. tab, graf. Resumo: The Mycobacterium tuberculosis-specific ESAT-6 antigen induces highly potent T-cell responses and production of gamma interferon (IFN-gamma), which play a critical role in protective cell-mediated immunity against tuberculosis (TB). In the present study, IFN-gamma secretion by peripheral blood mononuclear cells (PBMCs) in response to M. tuberculosis ESAT-6 in Brazilian TB patients was investigated in relation to clinical disease types, such as pleurisy and cavitary pulmonary TB. Leprosy patients, patients with pulmonary diseases other than TB, and healthy donors were assayed as control groups. Sixty percent of the TB patients indeed recognized M. tuberculosis ESAT-6, as did 50 per cent of the leprosy patients and 60 per cent of the non-TB controls. Nevertheless, the levels of IFN-gamma in response to the antigen ESAT, but not to antigen 85B (Ag85B) and purified protein derivative (PPD), were significantly lower in controls than in patients with treated TB or pleural or cavitary TB. Moreover, according to Mycobacterium bovis BCG vaccination status, only 59 per cent of the vaccinated TB patients responded to ESAT in vitro, whereas 100 per cent of them responded to PPD. Both CD4 and CD8 T cells were able to release IFN-gamma in response to ESAT. The present data demonstrate the specificity of ESAT-6 of M. tuberculosis and its ability to discriminate TB patients from controls, including leprosy patients. However, to obtain specificity, it is necessary to include quantitative IFN-gamma production in response to the antigen as well, and this might limit the use of ESAT-6-based immunodiagnosis of M. tuberculosis infection in an area of TB endemicity. (AU). Descritores: ANTIGENOS DE BACTERIAS/DU/GE/*IM LINFOCITOS T CD4-POSITIVOS/*IM LINFOCITOS T CD8-POSITIVOS/*IM INTERFERON TIPO II/BI MYCOBACTERIUM TUBERCULOSIS/*IM PROTEINAS RECOMBINANTES/IM TUBERCULINA/IM TUBERCULOSE PLEURAL/DI/*IM/MI TUBERCULOSE PULMONAR/DI/*IM/MI Limites: FEMININO HUMANO MASCULINO SUPPORT, NON-U.S. GOV'T Localização: BR191.1; 09070/s

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Id: 18514 Autor: Bala, Lakshmi; Anand, Sukumar; Sinha, Sudhir Título: Enhancement of human T cell response to a peptide epitope of 38kDa antigen of Mycobacterium tuberculosis by liposomes ..- Fonte: s.l; s.n; 2002. 9 p. graf. Resumo: Diagnosis of tuberculosis a problem, specially in the regions harboring an abundance of both pathogenic and non-pathogenic mycobacteria. This study was undertaken to assess in such a situation the predictive value of proliferative T cell response to a peptide epitope ('38G') of the 38 kDa membrane protein of Mycobacterium tuberculosis. 3[H]-thymidine incorporation assays were done with peripheral blood mononuclear cells of tuberculoid leprosy and pulmonary tuberculosis patients. The donors were also classified as PPD responders (Stimulation Index, SI> 3) or non-responders (SI < or = 3) on the basis of their T cell response to the 'Purified Protein Derivative (PPD)' of M. tuberculosis. 38G peptide was used in either free or liposome-associated form prepared by the technique of 'Dehydration-rehydration Vesicles' (Kirby and Gregoriadis, 1984). While free peptide failed to induce a positive response in study subjects, its liposomal form was T cell stimulatory and distinguished, to certain extent, between PPD responders (corresponding SI > 3 in 54 per cent subjects) and non-responders (SI > 3 in 29 per cent subjects). However, it did not differentiate between leprosy and tuberculosis. The study supports use of liposomes as adjuvant vehicles for antigenic peptides designed to activate human T cells. (AU). Descritores: ANTIGENOS DE BACTERIAS/admin ANTIGENOS DE BACTERIAS/quim ANTIGENOS DE BACTERIAS/genet HANSENIASE TUBERCULOIDE/diag HANSENIASE TUBERCULOIDE/imunol EPITOPOS/admin EPITOPOS/quim EPITOPOS/genet DIAGNOSTICO DIFERENCIAL MYCOBACTERIUM TUBERCULOSIS/quim MYCOBACTERIUM TUBERCULOSIS/genet MYCOBACTERIUM TUBERCULOSIS/imunol DADOS DE SEQUÊNCIA MOLECULAR SEQUÊNCIA DE AMINOACIDOS LINFOCITOS T/imunol TESTE TUBERCULINICO TUBERCULOSE PULMONAR/diag TUBERCULOSE PULMONAR/imunol TRANSFORMACAO LINFOCITICA - LIPOSSOMOS  PESO MOLECULAR Limites: ESTUDO COMPARATIVO HUMANO IN VITRO SUPPORT, NON-U.S. GOV'T Meio Eletrônico: - . Localização: BR191.1; 09061/s

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Id: 18512 Autor: Yassin, A; Shennawy, M. el; Enany G. el; Wassef, N. F; Shoeb, S Título: Leprosy of the upper respiratory tract. A clinical bacteriological, histopathological and histochemical study of twenty cases ..- Fonte: s.l; s.n; 1975. 7 p. ilus, tab. Resumo: Twenty cases clinically diagnosed as leprosy were thoroughly examined for E.N.T. lesions. These lesions were subjected to bacteriological, histopathological and histochemical studies. The results have been tabulated and discussed with special stress on some findings which are of help in diagnosing the disease. (AU). Descritores: EPITELIO/patol PARALISIA FACIAL/etiol HISTOCITOQUIMICA HANSENIASE/diag HANSENIASE/microbiol HANSENIASE/patol LARINGE/patol LINFOCITOS MYCOBACTERIUM LEPRAE/isol METAPLASIA/etiol MUCOSA NASAL/patol INFECCOES RESPIRATORIAS/microbiol INFECCOES RESPIRATORIAS/patol FARINGE/patol - RINITE ATROFICA/etiol  CELULAS PLASMATICAS Limites: HUMANO MASCULINO FEMININO CRIANÇA ADULTO MEIA-IDADE IDOSO ADOLESCENTE Meio Eletrônico: - . Localização: BR191.1. 9119/s

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Id: 18511 Autor: Kalaiselvi, K; Rajaguru, P; Palanivel, M; Usharani, M. V; Ramu, G Título: Chromosomal aberration, micronucleus and Comet assays on peripheral blood lymphocytes of leprosy patients undergoing multidrug treatment. (Mutagenesis, 17, 309-312, 2002) ..- Fonte: s.l; s.n; 2003. 1 p. tab. Resumo: To evaluate the genetic damage in leprosy patients, we carried out the alkaline Comet assay and chromosomal aberration (CA) and micronucleus (MN) tests in peripheral blood lymphocytes of 50 leprosy patients receiving multidrug treatment (MDT) and 50 healthy individuals. The Comet assay showed statistically higher mean values for length to width ratios of DNA mass (P < 0.01) and for mean frequencies of tailed cells (P < 0.001) in cells of leprosy patients than in those of controls. Similarly, the mean frequencies of micronucleated cells (per 1000 cytochalasin B-induced binucleated cells) were significantly greater (P < 0.001) in leprosy patients (19.92 +/- 2.564) than in controls (1.6 +/- 0.231). A statistically significant 10-fold increase in the frequency of CAs (11.16 +/- 0.411) was observed in leprosy patients compared with controls (1.28 +/- 0.242). In multiple regression analyses, when patients and controls were considered together, disease factor alone significantly influenced the genotoxicity markers. In the control group, age and alcohol consumption significantly influenced MN and length to width ratios and CA frequency, respectively. However, in MDT-treated leprosy patients none of the other confounding factors (sex, age, smoking and alcohol drinking) significantly affected the extent of genetic damage.(AU). Descritores: ENSAIO EM COMETA CONSUMO DE BEBIDAS ALCOOLICAS ABERRACOES CROMOSSÔMICAS HANSENIASE/sangue HANSENIASE/quimioter LINFOCITOS/ef drogas LINFOCITOS/fisiol TESTES PARA MICRONUCLEOS FATORES SEXUAIS TABAGISMO Limites: MASCULINO FEMININO ADULTO HUMANO Meio Eletrônico: - . Localização: BR191.1; 09120/s

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Id: 18482 Autor: Kalaiselvi, K; Rajaguru, P; Palanivel, M; Usharani, M. V; Ramu, G Título: Chromosomal aberration, micronucleus and Comet assays on peripheral blood lymphocytes of leprosy patients undergoing multidrug treatment ..- Fonte: s.l; s.n; 2002. 4 p. ilus, tab. Resumo: To evaluate the genetic damage in leprosy patients, we carried out the alkaline Comet assay and chromosomal aberration (CA) and micronucleus (MN) tests in peripheral blood lymphocytes of 50 leprosy patients receiving multidrug treatment (MDT) and 50 healthy individuals. The Comet assay showed statistically higher mean values for length to width ratios of DNA mass (P < 0.01) and for mean frequencies of tailed cells (P < 0.001) in cells of leprosy patients than in those of controls. Similarly, the mean frequencies of micronucleated cells (per 1000 cytochalasin B-induced binucleated cells) were significantly greater (P < 0.001) in leprosy patients (19.92 +/- 2.564) than in controls (1.6 +/- 0.231). A statistically significant 10-fold increase in the frequency of CAs (11.16 +/- 0.411) was observed in leprosy patients compared with controls (1.28 +/- 0.242). In multiple regression analyses, when patients and controls were considered together, disease factor alone significantly influenced the genotoxicity markers. In the control group, age and alcohol consumption significantly influenced MN and length to width ratios and CA frequency, respectively. However, in MDT-treated leprosy patients none of the other confounding factors (sex, age, smoking and alcohol drinking) significantly affected the extent of genetic damage. (AU). Descritores: CONSUMO DE BEBIDAS ALCOOLICAS ABERRACOES CROMOSSÔMICAS ENSAIO EM COMETA HANSENIASE/sangue HANSENIASE/quimioter LINFOCITOS/ef drogas LINFOCITOS/fisiol TESTES PARA MICRONUCLEOS FATORES SEXUAIS TABAGISMO Limites: HUMANO MASCULINO FEMININO ADULTO Meio Eletrônico: - . Localização: BR191.1; 09045/s

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Id: 18428 Autor: Bjune, Gunnar Título: Significance of immune reactions in leprosy ..- Fonte: s.l; s.n; Jun-Jul. 1980. 20 p. . Resumo: The disease Leprosy is a chronic infectious disease caused by Mycobacterium leprae. The only known natural host for this mycobacterium is the human being. A mycobacterial disease described in wild armadillos in Louisiana as being indistinguishable from leprosy (walsh et al. 1975), is of questionable significance for the epidemiology in humans (Fillice et al. 1977).(AU). Descritores: IMUNOSSUPRESSAO TECNICAS IMUNOLOGICAS LINFOCITOS T/imunol ERITEMA NODOSO/imunol HANSENIASE/genet HANSENIASE/imunol HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/cresc Limites: HUMANO Meio Eletrônico: - . Localização: BR191.1; 00262/s

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