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| Id: | 18514
| | Autor: | Bala, Lakshmi; Anand, Sukumar; Sinha, Sudhir
| | Título: | Enhancement of human T cell response to a peptide epitope of 38kDa antigen of Mycobacterium tuberculosis by liposomes
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| | Fonte: | s.l; s.n; 2002. 9 p. graf.
| | Resumo: | Diagnosis of tuberculosis a problem, specially in the regions harboring an abundance of both pathogenic and non-pathogenic mycobacteria. This study was undertaken to assess in such a situation the predictive value of proliferative T cell response to a peptide epitope ('38G') of the 38 kDa membrane protein of Mycobacterium tuberculosis. 3[H]-thymidine incorporation assays were done with peripheral blood mononuclear cells of tuberculoid leprosy and pulmonary tuberculosis patients. The donors were also classified as PPD responders (Stimulation Index, SI> 3) or non-responders (SI < or = 3) on the basis of their T cell response to the 'Purified Protein Derivative (PPD)' of M. tuberculosis. 38G peptide was used in either free or liposome-associated form prepared by the technique of 'Dehydration-rehydration Vesicles' (Kirby and Gregoriadis, 1984). While free peptide failed to induce a positive response in study subjects, its liposomal form was T cell stimulatory and distinguished, to certain extent, between PPD responders (corresponding SI > 3 in 54 per cent subjects) and non-responders (SI > 3 in 29 per cent subjects). However, it did not differentiate between leprosy and tuberculosis. The study supports use of liposomes as adjuvant vehicles for antigenic peptides designed to activate human T cells. (AU).
| | Descritores: | ANTIGENOS DE BACTERIAS/admin
ANTIGENOS DE BACTERIAS/quim
ANTIGENOS DE BACTERIAS/genet
HANSENIASE TUBERCULOIDE/diag
HANSENIASE TUBERCULOIDE/imunol
EPITOPOS/admin
EPITOPOS/quim
EPITOPOS/genet
DIAGNOSTICO DIFERENCIAL
MYCOBACTERIUM TUBERCULOSIS/quim
MYCOBACTERIUM TUBERCULOSIS/genet
MYCOBACTERIUM TUBERCULOSIS/imunol
DADOS DE SEQUÊNCIA MOLECULAR
SEQUÊNCIA DE AMINOACIDOS
LINFOCITOS T/imunol
TESTE TUBERCULINICO
TUBERCULOSE PULMONAR/diag
TUBERCULOSE PULMONAR/imunol
TRANSFORMACAO LINFOCITICA
- LIPOSSOMOS
PESO MOLECULAR
| | Limites: | ESTUDO COMPARATIVO
HUMANO
IN VITRO
SUPPORT, NON-U.S. GOV'T
| | Meio Eletrônico: | - .
| | Localização: | BR191.1; 09061/s |
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