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| Id: | 13416
| | Author: | Bjune, G
| | Title: | In vitro lymphocyte stimulation in leprosy; simultaneous stimulation with Mycobacterium leprae antigens and phytohaemagglutinin
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| | Source: | s.l; s.n; jun. 1979. 9 p. tab, graf.
| | Abstract: | Peripheral blood lymphocytes from 105 subjects with different forms of leprosy and healthy contacts of leprosy patients weere stimulated in vitro different preparations of mycobacterial antugens alone or in combination with a suboptimal dose of phytohaemagglutinin (PHA). In nearly all individuals sonicated leprosy bacilli and PHA together gave a lower 3H-thymidine incorporation than didi the same dose of PHA alone. There was no difference in the degree of inhibition seen in the different patient groups or the healthy contacts. High doses of whole, washed Mycobacterium leprae, combined with PHA led to an increased thymidine incorporation in borderline tuberculoid leprosy patients who had experienced a reversal reaction, and in healthy contacts with less than 6 months exposure did not show an augmentation of the PHA-induced thymidine incorporation. The inhibition exerted by sonicated M. leprae was dose-dependent, seen even with very low doses of antigen, and was not due to direct Cytotoxicity, M. bovis, strain BCG, was weakly suppressive effect. There was no correlation between the suppressive effective of M. leprae antigens and the other mtcobacteria neither was there any correlation with the responses to the mycobacterial antigens alone. Many lepromatous leprosy patients showed significant suppression of background incorporation with addition of M. leprae antigens. This paper discusses wheter the apparent `non-responsiveness` in lepromatous leprosy could be due to active suppressor mechanisms operative in vitro.(AU).
| | Descriptors: | ANTIGENOS DE BACTERIAS/imunol
ANTIGENOS DE SUPERFÍCIE/imunol
HANSENIASE/imunol
HANSENIASE/metab
TRANSFORMACAO LINFOCITICA
TIMIDINA/metab
FITOHEMAGLUTININAS/farmacol
MYCOBACTERIUM LEPRAE/imunol
RELACAO DOSE-RESPOSTA IMUNOLOGICA
LINFOCITOS/metab
| | Limits: | HUMANO
| | Electronic Medium: | http://www.ilsl.br
| | Location: | BR191.1; 00020/s |
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