Database : HANSEN Search on : BIOPSIA/UTIL [Subject descriptor] References found : 4 [refine] Displaying: 1 .. 4   in format [Detailed]

page 1 de 1

1 / 4

HANSEN

select to print

Id: 18419 Author: Patrocinio, Lucas Gomes; Goulart, Isabela Maria Bernardes; Goulart, Luiz Ricardo; Patrocinio, Jose Antonio; Ferreira, Frederico Regerio; Fleury, Raul Negrao Title: Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction ..- Source: s.l; s.n; 2005. 6 p. ilus, tab. Abstract: Several discoveries about leprosy indicate that Mycobacterium leprae transmission mainly accurs by inhalation, and the nose is major port of entry and exit. The present study evaluated the clinical application of PCR for detection of M. leprae DNA in nasal mucosa biopsies in untreated leprosy patients (52) and their contacts (99) from the State Reference Center in Sanitary Dermatology and Leprosy, Uberlandia, MG, Brazil. PCR detection of a 372-base pair DNA fragment from M. leprae was accomplished in 36 (69.2%) patients, from which 34 (91.9%) of them were multibacillaries. Furhermore, PCR was positive in 3 (16.7%) of 18 slit-skin smear negative, 4 (25.0%) of 16 skin lesion BI negative, 8 (33.3%) of 24 nasal mucosa BI negative patients, and 10 of 99 contacts (10.1%). The presence of bacilli in 10.1% of the contats may potentially reflect an occult leprosy, and reflect an occult leprosy, and these patients must be accompanied, followed by a chemoprophylaxy treatment. Considering all PCR results against clinical and BI classification of patients and controls, we have found a sensitivity of 69.2%, a specificity of 89.9% and an accuracy of 82.8%. It has been demonstrated here through PCR of nasal biopsies that the bacillus invades the mucosa, passing through the nasal inferior turbinate to reach peripheral blood. Therefore, the molecular investigation of invasive nasal biopsies by PCR tests has proven to be useful in defining patients of higher risk of transmission and risk-group contacts, which is an important step ti reach the World Health Organization objective towards the elimination of leprosy as a public health problem (AU). Descriptors: MUCOSA NASAL/imunol MUCOSA NASAL/les MUCOSA NASAL/microbiol MUCOSA NASAL/patol MYCOBACTERIUM LEPRAE/isol MYCOBACTERIUM LEPRAE/ultraest REACAO EM CADEIA DA POLIMERASE/estatíst REACAO EM CADEIA DA POLIMERASE/tend REACAO EM CADEIA DA POLIMERASE/util - BIOPSIA/estatíst  BIOPSIA/tend  BIOPSIA/util  HANSENIASE/clas  HANSENIASE/diag  HANSENIASE/microbiol  HANSENIASE/patol Limits: HUMANO Location: BR191.a; 09307/s

2 / 4

HANSEN

select to print

Id: 18329 Author: Fasal, P Title: Leprosy occurs everywhere ?- Source: San Francisco; s.n; 1965. 7p p. ilus. Abstract: Leprosy can occur at all ages, in both sexes and in every race. It can be found in any geographic area and can simulate many diseases, especially those affecting the skin. The lepromatous type indicates less host resistance than the tuberculoid type. Histopathologic examination of a properly stained specimen obtained by skin biopsy is the most important diagnostic procedure. Treatment consist of long-term chemotherapy with sulfone drugs. Descriptors: HANSENIASE/clas HANSENIASE/compl HANSENIASE/diag HANSENIASE/epidemiol HANSENIASE/imunol HANSENIASE/terap HANSENIASE/transm HANSENIASE DIMORFA/diag HANSENIASE TUBERCULOIDE/diag HANSENIASE VIRCHOWIANA/diag HANSENIASE/patol DAPSONA/admin DAPSONA/uso terap - DISTRIBUICAO ESPACIAL  ANTIGENO DE MITSUDA/admin  ANTIGENO DE MITSUDA/anal  BIOPSIA/util Limits: HUMANO Location: BR191.1; 00198/s

3 / 4

HANSEN

select to print

Id: 18202 Author: Walsh, Douglas S; Lane, Joshua E; Abalos, Rodolfo M; Myint, Khin Saw Aye Title: Tunel and limited immunophenotypic analysis of apoptosis in paucibacillary leprosy lesions ..- Source: s.l; s.n; 2004. 5 p. ilus. Abstract: Some mycobacterial infections, such as tuberculosis, are characterized by apoptosis in infected or by-stander mononuclear immune cells. For localized (paucibacillary, PB) and diseminated (multibacillary, MB) leprosy, characterized by polarized Thl-like vs, Th2-like immune responses, respectivelly little is known about lesional apoptosis. We analyzed sections of paraffin-embedded, untreated leprosy lesions from 21 patients by an indirect immunofluorescent terminal deoxynucleotide-transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay. Some TUNEL (+) PB sections were then reacted with phycoerythein-conjugated (red)antibodies against T cells, monocytes, or antigen-presenting (Langerhans) cells. TUNEL (+) bodies were detected in 9 of 16 PB lesions (56%) and in 1 of 5 MB lesions (20%). Some TUNELL (+) bodies in PB disease were CD3+ (T cell), as well as CD4+ (T-helper) or cd8+ (T-cytotoxic) Apoptosis characterizes PB and MB leprosy lesions and may be more freqeunt in PB disease. In PB disease, some TUNEL (+) bodies may derive from T cells (AU). Descriptors: HANSENIASE DIMORFA/imunol HANSENIASE VIRCHOWIANA/imunol HANSENIASE TUBERCULOIDE/imunol HANSENIASE/imunol IMUNOHISTOQUIMICA/util MYCOBACTERIUM LEPRAE/patogen HANSENIASE/patol HANSENIASE DIMORFA/patol HANSENIASE VIRCHOWIANA/patol HANSENIASE TUBERCULOIDE/patol - APOPTOSE/imunol  APOPTOSE/fisiol  IMUNOHISTOQUIMICA/normas  BIOPSIA/util Limits: HUMANO Location: BR191.1; 09200/s

4 / 4

HANSEN

select to print

Id: 9527 Author: Boros, Luis Fernando; Fleury, Raul Negrao; Assis, Gerson Francisco de; Ura, Somei Title: Diagnostico da amiloidose secundaria por biopsia de glandula salivar menor de mucosa labial em pacientes com hanseniase virchoviana Secondary amyloidosis diagnosis by biopsy of labial salivary gland in lepromatous leprosy patients- Source: Bauru; s.n; 1996. 8 p. ilus, tab. Descriptors: AMILOIDOSE GLANDULAS SALIVARES MENORES HANSENIASE PATOLOGIA BUCAL BIOPSIA BIOPSIA MUCOSA BUCAL Location: BR191.1; 06203/s

---

page 1 de 1

Refine the search

Database : HANSEN

Advanced form

Search for : Free form

		Search	in field
1			WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type
2	and or and not		WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type
3	and or and not		WordsTitle wordsAbstract wordsAuthorInstitutional authorSubject descriptorLimitsLanguageCountry, year publicationJournalType of literatureBibliographic levelMonography in seriesPublication typeConferenceUnique identifierLocationElectronic supportEntry dateRecord type

Search engine: iAH powered by WWWISIS

IAH - © BIREME/PAHO/WHO Latin American and Caribbean Center on Health Sciences Information