Database : HANSEN Search on : ELISA/METODOS [Subject descriptor] References found : 23 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19661 Author: Roche, Paul W; Failbus, Sarah S; Britton, Warwick J; Cole, Robert. Title: Rapid method for diagnosis of leprosy by measurements of antibodies to the M. leprae 35-kDa protein: comparison with PGL-1 antibodies detected by ELISA and "Dipstick" methods. Source: Int. J. Lepr;67(3):279-286, Sept., 1999. ilus, graf. Abstract: A new rapid immuno-chromatographic test card for the detection of antibodies to the Mycobacterium leprae 35-kD protein is described. The new assay is compared in the same group of subjects with a direct enzyme ELISA method for 35-kD antibodies and with assays for anti-phenolic glycolipid-I (PGL-I) antibodies using a standard ELISA as well as the recently described [quot ]dipstick[quot ] method. Good concordance was found between the rapid methods and the corresponding ELISA methods. The detection of untreated paucibacillary leprosy by the 35-kD test card was 59% compared with 27% for the PGL-I dipstick; however, the specificity for the 35-kD test card was 90% compared with 100% for the PGL-I dipstick in an endemic population. The potential application of these new, rapid serologic methods for the diagnosis of leprosy under field conditions is discussed. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/fisiol Anexina A3/imunol ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a07.pdf / en Location: BR191.1

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Id: 19245 Author: Wu, Qinxue; Yin, Yueping; Zhang, Liangfen; Chen, Xiaohong; Yu, Yanhau; Li, Zhicheng; Yu, Hua; Lu, Chengzhi; Feng, Suying; Li, Xiaojie; Huo, Wei; Ye, Ganyun. Title: A study on a possibility of predicting early relapse in leprosy using a ND-O-BSA based ELISA. Source: Int. J. Lepr;70(1):1-8, Mar.,2002. tab. Abstract: Serological methods have been used for detecting infection with Mycobacterium leprae. We have applied a serological test to explore the possibility it could detect a bacterial relapse among patients who have been cured with chemotherapy. More specifically we used an indirect enzyme-linked immunosorbant assay (ELISA) using the natural disaccharide (ND) of the phenolic glycolipid antigen of M. leprae linked to bovine serum albumin as antigen. Antibody levels were measured in sera from normal controls, active leprosy cases, cured leprosy patients, and relapsing leprosy patients. We correlated antibody levels with the type of leprosy, the bacterial index, and with relapse among cured leprosy patients. In our hands, the ND-ELISA, when applied to screening for infection with M. leprae, had excellent sensitivity, specificity, positive and negative predictive values, and both a low false positive rate and a low false negative rate. Antibody levels gradually increased among active patients from the tuberculoid to the lepromatous end of the leprosy spectrum. There was a year-by-year fall in antibody levels in patients responding to chemotherapy. Antibody levels and the bacterial index were correlated using the Spearman's rank correlation method. Serial antibody levels were measured in 666 leprosy patients after being cured with dapsone monotherapy. Over a three year follow up, 95 multibacillary patients became antibody positive and 12 of them had bacterial relapses of their disease. In contrast, among 335 cases that remained antibody negative, only one relapse was seen. Among 44 paucibacillary cured patients who became antibody positive, there was one relapse. There were 192 such patients who remained antibody negative and one relapsed. The risk of relapse is 6.7 times higher among cured multibacillary patients compared to cured paucibacillary patients. Overall, the cumulative relapse rate among antibody positive cases was 13.7%, compared to 0.4% among antibody negative patients. We conclude that the ND-ELISA is a useful tool both for screening for early infection with M. leprae and for predicting a relapse in cured patients, particularly in cured multibacillary patients. (AU)^ien. Descriptors: Hanseníase/imunol Hanseníase/fisiopatol ELISA/instrum ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n1/v70n1a01.pdf / en Location: BR191.1

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Id: 18159 Author: Arruda, Maria Sueli P; *.Coelho, Kunie I.R; *.Montenegro, Mario R*. Title: Experimental paracoccidioidomycosis of hamster inoculated in the cheek pouch ..- Source: Netherlands; s.n; 1994. 7 p. ilus, tab, graf. Abstract: We comparaed the granuloma morphology and immune response of hamsters inoculated with Paracoccidioides brasiliensis (Pb) into the cheek pouch, which lacks lymphatic drainage, and the ffotpad, which is rich in lymphatics. Our objective was to better understand the modulation of Pb granuloma in an immunocompetent animal inoculated in an immunologically privileged site. The humoral immune response (ELISA) and cell mediated immunity (footpad test) became positive on days 7 and 14, respectively in animal inoculated into footpad and on days 35 and 60 in animals inoculated into the pouch. Typical epithelioid granulomas were observed at both sites on day 14. The number of fungi gradually decreased from the beginning of the experiment in footpad lesions, but only after day 35 in pouch granulomas, when cell mediated immunity was detectable. The results indicate that typical epithelioid paracoccidioidomycotic granulomas may develop in the absence of a detectable immune response; however, they are incapable of controlling fungal reproduction. Lack of lymphatic drainage delays the appearance of a detectable immune response, but with time fungi escape from the pouch, elicit an immune response and reach other organs. Our results further indicate the importance of the lymphatics in the pathogenesis of paracoccidioidomycosis. (AU). Descriptors: PARACOCCIDIOIDOMICOSE/compl PARACOCCIDIOIDOMICOSE/diag PARACOCCIDIOIDOMICOSE/microbiol PARACOCCIDIOIDOMICOSE/fisiopatol GRANULOMA/microbiol GRANULOMA/fisiopatol BOCHECHA/anorm BOCHECHA/les BOCHECHA/microbiol - ELISA/instrum  ELISA/métodos  ELISA/tend  ELISA/vet Limits: ESTUDO COMPARATIVO CAMUNDONGOS Location: BR191.1; 09421/S

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Id: 12230 Author: Salotra, P; Sreenivas, G; Nasim, A. A; Subba Raju, B. V; Ramesh, V Title: Evaluation of enzyme-linked immunosorbent assay for diagnosis of post-kala-azar dermal leishmaniasis with crude or recombinant k39 antigen ..- Source: s.l; s.n; Mar. 2002. 4 p. tab, graf. Descriptors: ANTICORPOS ANTIPROTOZOARIOS ELISA ESTUDOS DE AVALIAÇAO LEISHMANIA DONOVANI LEISHMANIA DONOVANI LEISHMANIA DONOVANI LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL PROTEINAS DE PROTOZOARIOS SENSIBILIDADE E ESPECIFICIDADE Limits: ANIMAL Location: BR191.1; 08713/s

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Id: 11466 Author: Cho, Sang-Nae; Cellona, Roland V; Villahermosa, Laarni G; Fajardo Junior, Tranquilino T; Balagon, M. Victoria F; Abalos, Rodolfo M; Tan, Esterlina V; Walsh, Gerald P; Kim, Joo-Deuk; Brennan, Patrick J Title: Detection of phenolic glycolipid I of mycobacterium leprae in sera fron leprosy patients before and after start of multidrug therapy ..- Source: s.l; s.n; 2001. 5 p. ilus, tab, graf. Descriptors: ANTIGENOS DE BACTÉRIAS ELISA GLICOLIPIDIOS LEPROSTATICOS HANSENIASE HANSENIASE HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE ESTUDOS RETROSPECTIVOS Location: BR191.1; 08345/s

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Id: 11463 Author: Chatterjee, Mitali; Jaffe, Charles L; Sundar, Shyam; Basu, Debasis; Sen, Sandeep; Mandal, Chitra Title: Diagnostic and prognostic potential of a competitive enzyme-linked immunosorbent assay for leishmaniasis in India ..- Source: s.l; s.n; 1999. 5 p. ilus, tab, graf. Descriptors: ANTICORPOS MONOCLONAIS ANTICORPOS ANTIPROTOZOARIOS GLUCONATO DE ANTIMONIO E SODIO ANTIPROTOZOARIOS REAÇOES CRUZADAS ELISA INDIA LEISHMANIA DONOVANI LEISHMANIOSE CUTANEA LEISHMANIOSE CUTANEA LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL HANSENIASE HANSENIASE PROGNOSTICO MALARIA MALARIA TUBERCULOSE TUBERCULOSE Limits: ANIMAL Location: BR191.1; 08342/s

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Id: 11451 Author: Agdamag, Abraham T; Endoh, Masumi; Kawatsu, Kunio; Izumi, Shinzo Title: A serologic mycobacteria leprae gelatin particle agglutination (MLPA) in the diagnosis of leprosy: comparison with conventional enzyme-linked immunoassay and bacterial index ..- Source: s.l; s.n; 1996. 6 p. tab. Descriptors: TESTES DE AGLUTINAÇAO TÉCNICAS BACTERIOLOGICAS ELISA GELATINA HANSENIASE REPRODUTIBILIDADE DE RESULTADOS PELE Limits: ESTUDO COMPARATIVO Location: BR191.1; 08334/s

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Id: 10314 Author: Spierings, Eric; Vlieger, Monique de; Brand, Anneke; Klatser, Paul R; Ottenhoff, Tom H. M Title: Antibodies to sulfatide in leprosy and leprosy reactions ..- Source: s.l; s.n; 1999. 5 p. ilus, graf. Descriptors: ANTICORPOS ELISA HANSENIASE HANSENIASE HANSENIASE HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA HANSENIASE TUBERCULOIDE HANSENIASE TUBERCULOIDE IMUNOGLOBULINA G/BL IMUNOGLOBULINA M/BL SULFOGLICOESFINGOLIPIDEOS/*IM Location: BR191.1; 07416

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Id: 10206 Author: Brennan, Patrick J; Chatterjee, Delphi; Fujiwara, Tsuyoshi; Cho, Sang-Nae Title: Leprosy-specific neoglycoconjugates: synthesis and application to serodiagnosis of leprosy ..- Source: s.l; s.n; 1994. 11 p. ilus, tab. Descriptors: ANTIGENOS DE BACTÉRIAS SEQUENCIA DE CARBOIDRATOS ELISA GLICOCONJUGADOS GLICOCONJUGADOS GLICOLIPIDIOS GLICOLIPIDIOS IGM HANSENIASE HANSENIASE HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE Location: BR191.1; 06980/s

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Id: 10085 Author: Rafi, Abdolnasser Title: IgG response to some mycobacterial antigens in selected leprosy patients ..- Source: s.l; s.n; 1998. 5 p. tab, graf. Descriptors: HANSENIASE HANSENIASE HANSENIASE FORMAÇAO DE ANTICORPOS ANTIGENOS DE BACTÉRIAS ELISA GLICOLIPIDIOS IMUNOTERAPIA ATIVA MYCOBACTERIUM Location: BR191.1; 07144/s

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Id: 10009 Author: Klatser, Paul R Title: Laboratory techniques for diagnosis ..- Source: s.l; s.n; 1994. 3 p. . Descriptors: ELISA HANSENIASE HANSENIASE HANSENIASE REAÇAO EM CADEIA POR POLIMERASE SENSIBILIDADE E ESPECIFICIDADE TESTES CUTANEOS Location: BR191.1; 06905/s

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Id: 9793 Author: Kifayet, A; Shahid, F; Lucas, S; Hussain, R Title: Erythema nodosum leprosum is associated with up-regulation of polyclonal IgG1 antibody synthesis ..- Source: s.l; s.n; 1996. 7 p. ilus, tab, graf. Descriptors: HANSENIASE LEPROMATOSA ELISA ERITEMA NODOSO SOROS IMUNES UP-REGULATION (FISIOLOGIA) Location: BR191.1; 06755/s

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Id: 9583 Author: Pessolani, Maria Cristina Vidal; Peralta, Jose Mauro; Rumjanek, Franklin D; Gomes, Harrison Magdinier; Marques, Maria Angela de Melo; Almeida, Elza Carmen Cerqueira; Saad, Maria Helena Feres; Sarno, Euzenir Nunes Title: Serology and leprosy: immunoassays comparing immunoglobulin G antibody responses to 28 and 30 kilodalton proteins purified from mycobacterium bovis BCG ..- Source: s.l; s.n; 1991. 6 p. tab, graf. Descriptors: HANSENIASE MYCOBACTERIUM BOVIS ANTICORPOS ANTIBACTÉRIAS ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS ELISA GLICOLIPIDIOS PESO MOLECULAR Location: BR191.1; 06494/s

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Id: 9528 Author: Repka, Joao Carlos D; Fabricio, Lincoln H; Brand, Luiz Renato; Lemos, Alberto L; Penteado Filho, Sergio R; Stahlke, Paulo H Title: Imunologia na hanseniase: (II) estudo sorologico pelo metodo imunoenzimatico (ELISA), contra o glicoconjugado sintetico do mycobacterium leprae em contactos profissionais com hansenianos Immunology in leprosy: (II) serologic study bu enzyme linked immune sorbent assay (ELISA) against yhe synthetic glycoconjugate of mycobacterium leprae in professional contacts with leprosy patients- Source: s.l; s.n; 1991. 8 p. tab, graf. Descriptors: MYCOBACTERIUM LEPRAE HANSENIASE HANSENIASE HANSENIASE ELISA ELISA TESTES IMUNOLOGICOS TESTES IMUNOLOGICOS Location: BR191.1; 06205/s

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Id: 9498 Author: Pal, Abhijit; Mukerji, Krishna; Basu, Debashsih; Naskar, Kshudiram; Mallick, K. K; Ghosh, Dilip K Title: Evaluation of direct agglutination test (DAT) and ELISA for serodiagnosis of visceral leishmaniasis in India ..- Source: s.l; s.n; 1991. 4 p. ilus, tab. Descriptors: TESTES DE AGLUTINAÇAO DIAGNOSTICO DIFERENCIAL ELISA LEISHMANIOSE VISCERAL BAÇO Location: BR191.1; 06354/s

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Id: 9274 Author: Cho, Sang-Nae; Cellona, Roland V; Villahermosa, Laarni G; Fajardo Junior, Tranquilino T; Balagon, M. Victoria F; Abalos, Rodolfo M; Tan, Esterlina V; Walsh, Gerald P; Kim, Joo-Deuk; Brennan, Patrick J Title: Detection of phenolic glycolipid I of Mycobacterium leprae in sera from leprosy patients before and after start of multidrug therapy ..- Source: s.l; s.n; 2001. 5 p. ilus, tba, graf. Descriptors: HANSENIASE HANSENIASE HANSENIASE ANTIGENOS DE BACTÉRIAS ELISA LEPROSTATICOS MYCOBACTERIUM LEPRAE Location: BR191.1; 06258/s

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Id: 9248 Author: Madrid, Marta Cecilia Saenz Title: Deteccao sorologica de anticorpos anti-HIV 1 em pacientes com hanseniase ?- Source: Sao Paulo; s.n; 1996. 97 p. ilus, tab, graf. Thesis: Present for Universidade de Sao Paulo. Faculdade de Medicina presented for the degree Mestre. Abstract: Foi realizada a deteccao sorologica dos anticorpos anti-HIV 1 em um grupo de 59 pacientes com hanseniase multibacilar (Grupo A), 20 dos quais apresentaram surto reacional tipo II. Foi incluida tambem a analise retrospectiva da mesma sorologia correspondente a 104 pacientes (Grupo B) com formas multi e paucibacilares de hanseniase. As amostras de ambos os grupos foram testadas utilizando dois tipos de ELISA para o HIV 1. A prevalencia da infeccao pelo HIV 1 foi significativamente superior entre os pacientes com hanseniase (1,84 por cento) que entre os doadores de sangue (Grupo Controle - 0,28 por cento). Resultados falsamente positivos nao foram detectados nos testes de ELISA utilizados(AU). Descriptors: HANSENIASE ANTICORPOS ANTI-HIV ELISA Location: BR191.1; WC335.102, M267d

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Id: 9129 Author: Itano, Eiko N; Somens, Carlos C; Kaminami, Mari S; Ferreira, Claudete S. A; Mazzarim, Aparecida M; Vieira, Aline A Title: Caracterizacao parcial de receptores soluveis E presentes em soros de pacientes com hanseniase Partial characterization of E-soluble receptor (Rs) presente in Hansen's disease (HD) patients sera- Source: s.l; s.n; 1995. 6 p. ilus, graf. Descriptors: HANSENIASE ERITROCITOS ANTIGENOS CD2 SORO ANTILINFOCITARIO IMUNOSSUPRESSAO ELISA CROMATOGRAFIA Location: BR191.1; 06085/s

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Id: 9120 Author: Torrela, Adriana; Solis, Rosa L; Perez, Esther; Medina, Yadira; Kerguelen, Carlos; Olaya, Patricia Title: Anti M. leprae IgM antibody determination by ultramicroimmunoenzimatic (UMELISA HANSEN) for the diagnosis and monitoring leprosy ..- Source: Sao Paulo; s.n; 1998. 8 p. tab, graf. Descriptors: ANTICORPOS ANTIBACTÉRIAS ELISA GLICOPEPTIDIOS IGM HANSENIASE MYCOBACTERIUM LEPRAE OVINOS FATORES DE TEMPO Location: BR191.1; 06055/s

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Id: 8946 Author: Brasil, Mitie Tada L. R. F; Oliveira, Luiz Roberto de; Mello, Carmen Silva de; Nakamura, Paulo Mutuko; Manini, Marli P; Steiner, Denise; Rotta, Osmar. Title: Estudo da sensibilidade e especificidade do teste Elisa Anti PGL-1 no Estado de Sao Paulo / Sensibility study and specificity of ELISA test anti PGL-1 at Säo Paulo State Source: Hansen. Int;22(2):35-43, jul. - dez. 1997. tab, graf. Abstract: Os testes sorológicos para diagnóstico de hanseníase, usando o glicolipídeo-fenólico, considerado antígeno específico do M. leprae têm aberto algumas possibilidades de estudo do comportamento epidemiológico desta doença. Foi realisado um estudo para medir a sensibilidade e especificidade de um teste de ELISA anti PGL-1, usando material e técnica proveniente de Cuba (UMELISA). Foram testados 84 doentes e 112 controles sadios. A sensibilidade do teste foi maior para o grupo de doentes multibacilares, sendo mais alto entre os casos classificados como virchovianos (V), seguido dos dimorfos (D). No grupo de multibacilares, considerando o limiar de reatividade de 0,200, observou-se que apenas 75,0(por cento) de pacientes V e 50,0(por cento) de D foram positivos ao teste e, no limiar de 0,300, apenas 64,3(por cento) dos V e 40,0(por cento) dos D ainda mostravam positividade. Os doentes indeterminados apresentaram maior proporçäo de soropositivos do que os tuberculóides e isto talvez traduza a polarizaçäo de alguns casos para formas multibacilares. A especificidade do teste foi de 87,5(por cento) no limiar de 0,200 e de 99,1(por cento) no de 0,300. Este teste, à semelhança de outros, apresentou alta especificidade e baixa sensibilidade, resultando em grande percentual de falso-negativos. Para uma doença em que os testes sorológicos säo escassos, apesar de näo se recomendar o uso indiscriminado do teste para triagem de casos na populaçäo geral, a comunidade de seu aprimoramento tecnológico deve ser estimulada. Conseguir-se-ia, assim, avançar na pesquisa de instrumentos cada vez mais sensíveis e específicos para melhorar o diagnóstico precoce e, desse modo intervir mais oportunamente na cadeia de transmissäo. Descriptors: HANSENÍASE/imunologia COMPOSTOS FENÓLICOS/análise ELISA/instrumentação ELISA/métodos Limits: ESTUDO COMPARATIVO Electronic Medium: http://hansen.bvs.ilsl.br/textoc/hansenint/v21aov29/1997/PDF/v22n2/v22n2a05.pdf / pt Location: BR191.1

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