Database : HANSEN Search on : CULTURA [Subject descriptor] References found : 23 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 18453 Author: Venturini, James Title: Hanseniase Dimorfa: perfil de citocinas em sobrenadante de cultura de células mononucleares e in situ^ipt Leprosy bordeline: profile of cytokines in the culture supernatant of mononuclear cells and in situ- Source: Botucatu; s.n; 2008. 180 p. ilus, tab, graf. Thesis: Present for Universidade Estadual Paulista. Faculdade de Medicina de Botucatu presented for the degree Mestre. Abstract: Visando contribuir para o melhor entendimento da participação das citocinas na hanseníase dimorfa, o presente estudo investigou a produção desses mediadores in vitro e in situ em pacientes dimorfos-tuberculóides (HDT) e dimorfos-virchovianos(HDV). Foram avaliados 7 pacientes HDT e 12 HDV, virgens de tratamento, além de 19 indivíduos sádios (grupo controle). Culturas de células mononucleares do sangue periférico (PBMC) foram estimuladas ou não com estímulos inespecíficos e específicos do M. Leprae (antígeno inteiro e sonicado) e após 48 horas o sobrenadante foi recolhido para dosagens das citocinas TNF-a, IFN-y, IL-10 e TGF-B1. Biópsias das lesões cutâneas foram submetidas aos procedimentos histológicos por meio da coloração com hematoxilina-Eosina e Faraco-Fite; os cortes foram submetidos, ainda, à detecção in situ de iNOS, IL-10 e TGF-B1 por imunoistoquímica. A quantificação de citocinas em sobrenadante de PBMC revelou que pacientes HDT produziram níveis menores de TGF-B1 e pacientes HDV, níveis menores de IL-10...(AU)^ipt. Descriptors: Hanseníase Dimorfa/imunol Hanseníase Dimorfa/patol Técnicas de Cultura de Células/métodos Citocinas/bios Citocinas/imunol - Interleucina-10/bios  Interleucina-10/imunol Limits: Humanos Location: BR191.1; WC335.102.32, V568h

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Id: 18235 Author: Roy, A. N Title: Some interesting observations on the cultivation Mycobacterium leprae ..- Source: s.l; s.n; 1966. 2 p. . Abstract: A preliminary report on cultivation of Mycobacterium leprae in artificial media is reported.The work suggests that the modified medium used in this work may help in cultivating Mycobacteria leprae (AU). Descriptors: MYCOBACTERIUM LEPRAE/cresc MYCOBACTERIUM LEPRAE/isol MEIOS DE CULTURA/quim - MICROSCOPIA/util Limits: IN VITRO Location: BR191.1; 09280/s

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Id: 16607 Author: Hanks, John H. Title: Behavior of leprosy bacilli in complex liquid media with highly available sources of nutrient and acessory substances. Source: Int. J. Lepr;9(3):275-298, July-Sept. 1941. tab. Abstract: Completely homogenized suspensions of fifteen leprous nodules were inoculated into complex natural media such as blood, serum, leprous tissue extracts, chick embryo juice, and sterile egg yolk suspensions. These substances were supplemented with a variety of proteolytic digests, autolysates, peptones, products derived from acid-fast bacilli, yeast extract and simpler sources of nitrogen and carbon. Each inoculated medium also contained all of the nutrient substances present in the lepromata. Diphtheroid bacilli were recovered from three of six cases showing ulcers of the skin. One culture of a streptothrix and two of acid-fast bacilli were also obtained from one of the ulcerated cases. A third acid-fast microorganism was cultivated from a patient who showed no ulcers. These acid-fast cultures were not tubercle bacilli. By quantitative microscopic studies in liquid media from which aliquot portions were removed for comparison with control smears or formalinized suspensions, it was learned that the leprosy bacilli did not multiply in any of the 109 nutritional combinations tested, or in the presence of added carbon dioxide and oxygen. Growth did not occur in media shown to be suitable for the cultivation of human blood monocytes or of fibroblasts from lepromata. (AU). Descriptors: MYCOBACTERIUM LEPRAE/isol MEIOS DE CULTURA Location: BR191.1

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Id: 16500 Author: Degotte, J; Dubois, A. Title: Epidemiological leprosy survey in the Nepoko, Kibali-Ituri District, Belgian Congo. Source: Int. J. Lepr;8(4):421-444, Oct.-Dec. 1940. ilus, mapas, tab, graf. Descriptors: HANSENIASE/clas HANSENIASE/etnol HANSENIASE/epidemiol HANSENIASE/genet HANSENIASE/hist ISOLAMENTO DE PACIENTES/hist - TOPOGRAFIA MEDICA  CONDICOES SOCIAIS  CULTURA Limits: HUMANO Location: BR191.1

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Id: 13501 Author: Haregewoin, Abebe; Godal, Tore; Mustafa, Abu Salim; Belehu, Ayele; Yemaneberhan, Tabebe Title: T-cell conditioned media reverse T-cell unresponsiveness in lepromatous leprosy ..- Source: s.l; s.n; May 26, 1983. 3 p. . Abstract: In some subjects the infective agent of leprosy, Mycobacterium leprae, causes disseminated (lepromatous) disease. Such subjects have a major role in the transmission of the disease and show deficient T-cell responses both in vivo and in vitro to M. leprae, but not to other antigens. Numerous studies have recently shown that T cells with functional capabilities after initial triggering with antigen can be maintained in a state of continuous proliferation in vitro when cultured in medium containing interleukin 2 (IL-2). Here we have studied the effect of IL-2 rich T-cell conditioned medium on lepromatous peripheral blood mononuclear cells. Our results show that although lepromatous T cells fail to produce IL-2 after exposure to M. leprae they can respond by proliferation to M. leprae in the presence of T-cell conditioned medium, suggesting that the unresponsiveness in lepromatous leprosy results from a deficiency in the production of IL-2 or related factors and not a lack of M. leprae-reactive T cells.(AU). Descriptors: MEIOS DE CULTURA TOLERÂNCIA IMUNOLOGICA INTERLEUCINA-2/imunol HANSENIASE/imunol TRANSFORMACAO LINFOCITICA LINFOCITOS T/imunol Limits: HUMANO SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 01401/s

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Id: 13415 Author: Nelson, D. S; Penrose, J. M; Waters, M. F; Pearson, J. M; Nelson, M Title: Depressive effect of serum from patients with leprosy on mixed lymphocyte reactions ..- Source: s.l; s.n; dec. 1975. 8 p. tab. Abstract: Mixed leucocyte cultures, from two normal donors, were set up in media containing human serum from one of the following sources: (a) a pool of normal group AB donors; (b) Chinese, Malay or Indian patients with untreated leprosy; (c) the same patients after effective anti-leprosy treatment; (d) control Chinese, Malay or Indian subjects. Transformation was estimated by measuring the incorporation of tritiated thymidine in the last 24 hr of a 7-day culture period. Transformation was impaired in sera from treated lepromatous patients, but was less impaired or not impaired at all in sera from treated lepromatous patients. The loss of depressive activity after treatment was more marked in Chinese and Indian than in Malay patients. Transformation was also impaired, though to a lesser extent, in sera from patients with untreated tuberculoid leprosy; it was still impaired in sera from treated tuberculoid patients. There was no evidence of specificity in impairment of mixed lymphocyte reactivity and lymphocytotoxic antibodies appeared to play no role. The incidences of hepatitis B antigen and antibody and of anti-nuclear factor were not notably high.(AU). Descriptors: ANTICORPOS ANTINUCLEARES ESPECIFICIDADE DE ANTICORPOS DAPSONA/uso terap GRUPOS ETNICOS ANTIGENOS DA HEPATITE B HANSENIASE/quimioter HANSENIASE/imunol TESTE DE CULTURA MISTA DE LINFOCITOS TRANSFORMACAO LINFOCITICA Limits: HUMANO MASCULINO FEMININO CRIANÇA ADULTO MEIA-IDADE IDOSO ADOLESCENTE Electronic Medium: http://www.ilsl.br Location: BR191.1; 00021/s

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Id: 13315 Author: Haregewoin, Abebe; Godal, Tore; Mustafa, Abu Salim; Belehu, Ayele; Yemaneberhan, Tabebe Title: T-cell conditioned media reverse T-cell unresponsiveness in lepromatous leprosy ..- Source: s.l; s.n; may 1983. 3 p. ilus, tab. Abstract: In some subjects the infective agent of leprosy, Mycobacterium leprae, causes disseminated (lepromatous) disease. Such subjects have a major role in the transmission of the disease and show deficient T-cell responses both in vivo and in vitro to M. leprae, but not to other antigens. Numerous studies have recently shown that T cells with functional capabilities after initial triggering with antigen can be maintained in a state of continuous proliferation in vitro when cultured in medium containing interleukin 2 (IL-2). Here we have studied the effect of IL-2 rich T-cell conditioned medium on lepromatous peripheral blood mononuclear cells. Our results show that although lepromatous T cells fail to produce IL-2 after exposure to M. leprae they can respond by proliferation to M. leprae in the presence of T-cell conditioned medium, suggesting that the unresponsiveness in lepromatous leprosy results from a deficiency in the production of IL-2 or related factors and not a lack of M. leprae-reactive T cells.(AU). Descriptors: MEIOS DE CULTURA TOLERÂNCIA IMUNOLOGICA INTERLEUCINA-2/imunol HANSENIASE/imunol LINFOCITOS T/imunol - TRANSFORMACAO LINFOCITICA Limits: HUMANO SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 01176/s

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Id: 12244 Author: Rambukkana, Anura; Zanazzi, George; Tapinos, Nikos; Salzer, James L Title: Contact-dependent demyelination by mycobacterium leprae in the absence of immune cells ..- Source: s.l; s.n; May 2002. 5 p. ilus, graf. Descriptors: APOPTOSE AXONIOS AXONIOS LINFOCITOS B ADERENCIA BACTERIANA DIVISAO CELULAR COCULTURA DOENÇAS DESMIELINIZANTES GANGLIOS ESPINHAIS GENES RAG-1 GLICOLIPIDIOS HANSENIASE HANSENIASE HANSENIASE HANSENIASE MACROFAGOS CAMUNDONGOS CAMUNDONGOS ENDOGAMICOS C57BL CAMUNDONGOS KNOCKOUT MUTAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE BAINHA DE MIELINA BAINHA DE MIELINA DEGENERAÇAO NEURAL FIBRAS NERVOSAS MIELINIZADAS NEURONIOS CÉLULAS DE SCHWANN CÉLULAS DE SCHWANN NERVO CIATICO NERVO CIATICO LINFOCITOS T CULTURA DE TECIDO Limits: ANIMAL Location: BR191.1; 08647/s

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Id: 11749 Author: Rambukkana, Amura; Zanazzi, George; Tapinos, Nikos; Salzer, James L Title: Contact-dependent demyelination by Mycobacterium leprae in the absence of immune cells ..- Source: s.l; s.n; 2002. 5 p. ilus. Descriptors: HANSENIASE HANSENIASE HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE AXONIOS AXONIOS ADERENCIA BACTERIANA DIVISAO CELULAR COCULTURA DOENÇAS DESMIELINIZANTES GANGLIOS ESPINHAIS GENES RAG-1 GLICOLIPIDIOS MACROFAGOS MUTAÇAO BAINHA DE MIELINA BAINHA DE MIELINA DEGENERAÇAO NEURAL - FIBRAS NERVOSAS MIELINIZADAS  NEURONIOS  CÉLULAS DE SCHWANN  CÉLULAS DE SCHWANN  NERVO CIATICO  NERVO CIATICO  LINFOCITOS T  CULTURA DE TECIDO Location: BR191.1; 08525/s

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Id: 11029 Author: Elissen, Marielle C. C. A Title: Beliefs of leprosy patients about their illness: a study in the province of South Sulawesi, Indonesia ..- Source: s.l; s.n; 1991. 4 p. . Descriptors: ATITUDE FRENTE A SAUDE CULTURA INDONÉSIA ISLAMISMO HANSENIASE QUESTIONARIOS Location: BR191.1; 07207/s

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Id: 10162 Author: Ishaque, M; Sticht-Groh, V; Tologa, D Title: Susceptibility of severe combined immunodeficient mice to Mycobacterium leprae ..- Source: s.l; s.n; 1996. 8 p. tab. Descriptors: CONTAGEM DE COLONIA MICROBIANA MEIOS DE CULTIVO CONDICIONADOS MEIOS DE CULTURA PÉ HOSPEDEIRO IMUNOCOMPROMETIDO HANSENIASE HANSENIASE CAMUNDONGOS CAMUNDONGOS SCID MYCOBACTERIUM LEPRAE CAMUNDONGOS NUS Limits: ESTUDO COMPARATIVO ANIMAL Location: BR191.1; 07075/s

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Id: 10081 Author: Dhople, Arvind M Title: Factors influencing the in vitro growth of Mycobacterium leprae: effect of inoculum ..- Source: s.l; s.n; 1998. 11 p. tab. Descriptors: HANSENIASE HANSENIASE ADENOSINA TRIFOSFATO RADIOISOTOPOS DE COBALTO MEIOS DE CULTURA FIGADO FIGADO BAÇO BAÇO TIMIDINA MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE Location: BR191.1; 07140/s

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Id: 9974 Author: Kato, L; Szejtli, J; Szente, L Title: Water soluble complex of palmitic acid in media for cultivation of leprosy-derived psychrophilic mycobacteria from mycobacterium leprae infected tissues ..- Source: s.l; s.n; 1993. 12 p. tab. Descriptors: ANIMAIS TATUS TÉCNICA DE TIPAGEM BACTERIANA MEIOS DE CULTURA TESTES INTRADÉRMICOS HANSENIASE HANSENIASE CAMUNDONGOS MYCOBACTERIUM MYCOBACTERIUM MICOBACTERIOSE MICOBACTERIOSE MYCOBACTERIUM LEPRAE ACIDOS PALMITICOS SOLUBILIDADE TEMPERATURA AMBIENTE Location: BR191.1; 06618/s

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Id: 9694 Author: Gailly, Ch; David, Fr; Sandra, P; Laneelle, M. A; Cocito, C Title: Lipid composition of leprosy-derived corynebacteria, a distinct group of corynebacteria, and of a reference corynebacterium ..- Source: s.l; s.n; 1993. 18 p. ilus, tab, graf. Descriptors: PAREDE CELULAR CROMATOGRAFIA EM CAMADA DELGADA CORYNEBACTERIUM CORYNEBACTERIUM CORYNEBACTERIUM MEIOS DE CULTURA ACIDOS GRAXOS FRACIONAMENTO HANSENIASE LIPIDIOS LIPIDIOS FRAGMENTOMETRIA DE MASSA ACIDOS MICOLICOS ACIDOS ESTEARICOS Limits: ESTUDO COMPARATIVO Location: BR191.1; 06656/s

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Id: 9503 Author: Dhople, Arvind Madhav; Lamoureux, Laurie Christine Title: Factors influencing the in vitro growth of Mycobacterium leprae: effect of oxygen ..- Source: s.l; s.n; 1991. 8 p. tab. Descriptors: ADENOSINA TRIFOSFATO AEROBIOSE CONTAGEM DE COLONIA MICROBIANA MEIOS DE CULTURA MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM PHLEI MYCOBACTERIUM PHLEI OXIGENIO CONSUMO DE OXIGENIO TIMIDINA Limits: ESTUDO COMPARATIVO Location: BR191.1; 06359/s

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Id: 6925 Author: Kato, Lazlo. Title: Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from Mycobacterium leprae infected tissues. Source: Hansen. Int;19(1):17-27, jun. 1994. tab. Abstract: Cultivation trials for Mycobacterium leprae resulted in growth of Mycobacterium psychrophilum (L). Media were inoculated with host grown Mycobacterium leprae cells from armadillo tissues, Nu mice foot pads or human lepromata. Cultures were obtained in liquid and on semisolid multifactoria 1 media containing water soluble palmitic acid or its salts. Ammonium thioglycolate and Napalmitate served as carbon and energy sources. The water soluble palmitic acid remained in perfect solution following sterilization in the autoclave, thus easily accessible to the cells. The cyclodextrin-Fe complex served as a siderophore to grow the obtained leprosy derived psychrophilic cells. The leprosy derived cultures and subcultures grew opimally at+10 degrees Celssius but deteriorated rapidly at + 32 degrees Celsius, in the multifactorial media. No growth occurred in 7H9 media...(AU). Descriptors: HANSENIASE - MYCOBACTERIUM LEPRAE  MYCOBACTERIUM LEPRAE  MEIOS DE CULTURA  CULTURA Electronic Medium: http://hansen.bvs.ilsl.br/textoc/hansenint/v01aov20/1994/PDF/v19n1/v19n1a03.pdf / en Location: BR191.1

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Id: 6842 Author: Souza-Araujo, Heraclides Cesar de Title: Studies upon leprosy. II. Attempts to cultivate the Mycobacterium leprae (Coccothrix leprae Lutz, 1886). Isolating an actinomyces from a leproma. The actinomyces lepromatis ..- Source: s.l; s.n; 1928. 8 p. . Descriptors: HANSENIASE - MYCOBACTERIUM LEPRAE  CULTURA Location: BR191.1; 05167/s

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Id: 6839 Author: Souza-Araujo, Heraclides Cesar de Title: Cultura nao-cromogenica de um bacilo acido-alcool-resistente isolado do muco nasal de uma leprosa. Sua virulencia para animais de laboratorio ..- Source: s.l; s.n; 1952. 10 p. ilus. Descriptors: HANSENIASE - CULTURA  VIRULENCIA Location: BR191.1; 05134/s

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Id: 3767 Author: Massalski, Wandalin Kazimierz; Shukla, Rahma Raksha; Lewenstein, Wieslaw; Wierzbicki, Rafal Title: Culture of Hansen's bacilli in vitro in a case of lepromatous leprosy ..- Source: s.l; s.n; 1978. 2 p. ilus. Descriptors: HANSENIASE MEIOS DE CULTURA MYCOBACTERIUM LEPRAE Limits: HUMANO RELATO DE CASO ADOLESCENCIA Location: BR191.1; 02606/s

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Id: 3763 Author: Turcotte, R; Ishaque, M Title: Impairment of virulence of in vitro subcultures of Mycobacterium lepraemurium ..- Source: s.l; s.n; 1983. 3 p. tab. Descriptors: HANSENIASE MYCOBACTERIUM LEPRAEMURIUM MICOBACTERIOSE MICOBACTERIOSE TÉCNICAS BACTERIOLOGICAS MEIOS DE CULTURA CAMUNDONGOS ENDOGAMICOS C3H CAMUNDONGOS ENDOGAMICOS C57BL ESPECIFICIDADE DE ESPÉCIES VIRULENCIA Location: BR191.1; 02602/s

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