Database : HANSEN Search on : PROTEINAS [Subject descriptor] References found : 139 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19934 Author: Roger, Michel; Levee, Geraldine; Chanteau, Suzanne; Gicquel, Brigitte; Schurr, Erwin. Title: No evidence for linkage between leprosy susceptibility and the human natural resistance-associated macrophage protein 1 (NRAMP1) gene in French Polynesia. Source: Int. J. Lepr;65(2):197-202, Jun. 1997. tab. Abstract: In order to determine whether a human homolog (NRAMP1) to a murine candidate gene for resistance to mycobacteria influences susceptibility to human disease, we analyzed data from seven multicase leprosy families (84 individuals) from French Polynesia for linkage markers within the NRAMP1 gene and leprosy per se. Individual family members were typed at nine polymorphic loci within NRAMP1. In addition, three physically linked, polymorphic microsatellite markers-D2S104, D2S173 and D2S1471-were also typed. Linkage analyses were done using affected sibpair and LOD score methods employing different modes of inheritance with full and reduced penetrance. The results of this study strongly suggest that NRAMP1 is not linked to leprosy susceptibility in the French Polynesian families tested. (AU)^ien. Descriptors: Hanseníase/epidemiol Hanseníase/genet Proteínas de Transporte/genet Proteínas de Membrana/genet Limits: Humanos Masculino Feminino Location: BR191.1

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Id: 19620 Author: Thomas, Mathew M; Jacob, Mary; Chandi, Sushil M; George, Soshamma; Pulimood, Susanne; Jeyaseelan, L; Job, Charles K. Title: Role of S-100 staining in differentiating leprosy from other granulomatous diseases of the skin. Source: Int. J. Lepr;67(1):1-5, Mar., 1999. ilus, tab, graf. Abstract: Since Mycobacterium leprae are rarely demonstrable in the tuberculoid spectrum of leprosy, a confirmatory diagnosis of leprosy can be made on the basis of finding active destruction of cutaneous nerves by granulomatous inflammation in a skin biopsy. Immunoperoxidase staining for S-100 protein, which is a marker for Schwann cells, was used to delineate nerves in lesional skin biopsies of 25 patients with tuberculoid and borderline tuberculoid leprosy as well as 15 controls with nonleprous granulomatous inflammation. Four different patterns of nerve damage were observed: infiltrated, fragmented, absent, and intact. All of the nonleprous granulomatous dermatoses showed only intact nerves, either inside or outside the granuloma, and so S-100 staining can be used to rule out leprosy. (AU)^ien. Descriptors: Proteínas S100/imunol Hanseníase/imunol Hanseníase/fisiopatol Doença Granulomatosa Crônica/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n1/v67n1a01.pdf / en Location: BR191.1

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Id: 19364 Author: Suneetha, Lavanya M; Vardhini, Deena; Suneetha, Sujai; Balasubramanian, A. S; Job, C. K; Scollard, David. Title: Biochemical aspects of mycobacterium leprae binding proteins: a review of their role in pathogenesis. Source: Int. J. Lepr;69(4):341-348, Dec., 2001. tab. Descriptors: Mycobacterium leprae/quim Mycobacterium leprae/genet Mycobacterium leprae/imunol Proteínas/quim Proteínas/sint quim Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n4/v69n4edt.pdf / en Location: BR191.1

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Id: 19362 Author: Gupta, U. D; Katoch, K; Sharma, R. K; Singh, H. B; Natragan, M; Singh, D; Sharma, V. D; Chauhan, D. S; Das, Ram; Srivastava, K; Katoch, V. M. Title: Analysis of quantitative relationship between viability determination in leprosy by MFP, ATP bioluminescence and gene amplification assay. Source: Int. J. Lepr;69(4):328-334, Dec., 2001. tab. Abstract: Two hundred twenty-one untreated, borderline lepromatous/lepromatous (BL/LL) leprosy patients have been investigated for viability by the mouse foot pad method (MFP), adenosine triphosphate (ATP) and polymerase chain reaction (PCR). The biopsies were collected at the beginning of and 12/24 months after treatment. The patient group was treated with a) immunotherapy (BCG/Mw) + MDT; b) MDT + pyrazinamide; c) control MDT; d) MDT + minocycline 100 mg once a month supervised + ofloxacin 400 mg once a month supervised. Biopsies were divided in three parts for use in the mouse foot pad, molecular and ATP investigations. In untreated and treated patients (at 12 and 24 months), there was a general agreement among all three techniques, and PCR and ATP showed higher positivity as compared to MFP. Further, there was good correlation among the viable biomass estimated by bacillary ATP levels, PCR assay and growth in mouse foot pads. The positivity was observed by MFP as well as PCR assay (18-kDa and 36-kDa) from all of the specimens when the ATP content was more than 3.6 pg/million. When the ATP content was below 3.5 pg/million, the positive takes in MFP decreased but the PCR positivity correlated with ATP bioluminescence up to 0.04 pg/million. When the ATP content was even lower, the uptake in the MFP was possibly a matter of chance, while PCR positivity was observed in 96% of the cases. For specimens with undetectable ATP, positivity was seen in 1% of the cases, showing the inability of ATP bioluminescence method to detect low background due to host ATP. PCR signals in some cases could be due to the higher sensitivity of the method or persistence of DNA after bacterial death in some cases. On the whole, the PCR methods even though targeting DNA have shown good correlations with biomass which confirm their usefulness in monitoring therapeutic responses in leprosy. (AU)^ien. Descriptors: Análise Quantitativa/métodos Trifosfato de Adenosina/uso diag Trifosfato de Adenosina/imunol Proteínas Luminescentes/uso diag Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n4/v69n4a04.pdf / en Location: BR191.1

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Id: 19266 Author: Lew, Wook; Chang, Soo Kyoung; Kwahck, Ho; Tada, Yayoi; Nakamura, Koichiro; Tamaki, Kunihiko. Title: Serum monocyte chemoattractant protein-1 is elevated in lepromatous leprosy patients with high bacterial indices. Source: Int. J. Lepr;70(2):129-131, Jun. 2002. graf. Descriptors: Proteínas Quimioatraentes de Monócitos/imunol Proteínas Quimioatraentes de Monócitos/fisiol Hanseníase Virchowiana/imunol Hanseníase Virchowiana/fisiopatol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2002/pdf/v70n2/v70n2cor06.pdf / en Location: BR191.1

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Id: 18226 Author: Vardhini, Deena; Suneetha, Sujai; Ahmed, Niyaz; Joshi, D. S. M; Karuna, S; Magee, X; Vijayalakshmi, D. S. R; Sridhar, V; Karunakar, K. V; Archelos, Juan J; Suneetha, Lavanya M Title: Comparative proteomics of the Mycobacterium leprae binding protein myelin P0: its implication in leprosy and other neurodegenerative diseases ..- Source: s.l; s.n; 2004. 8 p. ilus, tab. Abstract: Mycobacterium leprae, the causative agent of leprosy invades Schwann cells of the peripheral nerves leading to nerve damage and disfigurement, which is the hallmark of the disease. Wet experiments have shown that M. leprae binds to a major peripheral nerve protein, the myelin P zero (P0). This protein is specific to peripheral nerve and may be important in the initial step of M. leprae binding and invasion of Schwann cells which is the feature of leprosy. Though the receptors on Schawann cells, cytokines, chemokines and antibodies to M. leprae have been identified the molecular mechanism of nerve damage and neurodegeneration is not clearly defined. Recently pathogen and host protein/nucleotide sequence similarities (molecular mimicry) have been implicated in neurodegenerative diseases. The approach of the present study is to utilise bioinformatic tools to understand leprosy nerve damage by carrying out sequence and structural similarity searches of myelin P0 with leproma and other genomic database. Since myelin P0 is unique to peripheral nerve, its sequence and structural similarities in other neuropathogens have also been noted. Comparison of myelin P0 with the M. leprae proteins revealed two characterised proteins, Ferrodoxin NADP reductase and a conserved membrane protein, which showed similarity to the query sequence. Comparison with the entire genomic database (www.ncbi.nlm.nih.gov) by basic local alignment search tool for proteins (BLASTP) and fold classification of structure-structure alignment of proteins (FSSP) searches revealed that myelin P0 had sequence/structural similarities to the poliovirus receptor, coxsackie-adenovirus receptor, anthrax protective antigen, diphtheria toxin, herpes simplex virus, HIV gag-1 peptide, and gp120 among others. These proteins are known to be associated directly or indirectly with neruodegeneration. Sequence and structural similarities to the immunoglobin regions of myelin P0 could have implications in host-pathogen interactions, as it has homophilic adhesive properties. Although these observed similarities are not highly significant in their percentage identity, they could be functionally important in molecular mimicry, receptor binding and cell signaling events involved in neurodegeneration. (AU). Descriptors: Sequência de Aminoácidos Proteínas de Bactérias/GE/ME Biologia Computacional Hanseníase/*ME/MI Proteínas de Membrana/* Modelos Moleculares Mimetismo Molecular Dados de Sequência Molecular Mycobacterium leprae/*GE/ME Proteína P0 da Mielina/CH/*GE/ME Doenças Neurodegenerativas/*ME Ligação Proteica Conformação Proteica Proteômica/* Receptores Virais/CH/ME Limits: HUMANO Location: BR191.1; 09195/S

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Id: 18184 Author: Block, Walter D Title: Serum electrophoretic studies on patients with familial primnary systemic amyloidosis ..- Source: s.l; s.n; 1956. 8 p. ilus, tab. Descriptors: Amiloidose Familiar/diag Amiloidose Familiar/imunol ELETROFORESE DAS PROTEINAS SANGUÍNEAS/métodos ELETROFORESE DAS PROTEINAS SANGUÍNEAS/estatíst - SOROGLOBULINAS/anal  PROTEINAS SANGUÍNEAS/anal Limits: HUMANO Location: BR191.1; 00857/s

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Id: 17691 Author: Scollard, D. M; Adams, L. B; Gillis, T. P; Krahenbuhl, J. L; Truman, R. W; Williams, D. L Title: The continuing challenges of leprosy ..- Source: s.l; s.n; 2006. 44 p. ilus, tab. Abstract: Leprosy is best understood as two conjoined diseases. The first is a chronic mycobacterial infection that elicits an extraordinary range of cellular immune responses in humans. The second is a peripheral neuropathy that is initiated by the infection and the accompanying immunological events. The infection is curable but not preventable, and leprosy remains a major global health problem, especially in the developing world, publicity to the contrary notwithstanding. Mycobacterium leprae remains noncultivable, and for over a century leprosy has presented major challenges in the fields of microbiology, pathology, immunology, and genetics; it continues to do so today. This review focuses on recent advances in our understanding of M. leprae and the host response to it, especially concerning molecular identification of M. leprae, knowledge of its genome, transcriptome, and proteome, its mechanisms of microbial resistance, and recognition of strains by variable-number tandem repeat analysis. Advances in experimental models include studies in gene knockout mice and the development of molecular techniques to explore the armadillo model. In clinical studies, notable progress has been made concerning the immunology and immunopathology of leprosy, the genetics of human resistance, mechanisms of nerve injury, and chemotherapy. In nearly all of these areas, however, leprosy remains poorly understood compared to other major bacterial diseases. (AU). Descriptors: Antiinfecciosos/TU Proteínas de Bactérias/ME Vacinas Bacterianas Modelos Animais de Doenças Suscetibilidade à Doença/IM Resistência Bacteriana a Drogas Genes Bacterianos/GE Predisposição Genética para Doença Genoma Bacteriano Imunidade Celular Imunidade Natural/GE Hansenostáticos/PD/TU Hanseníase/*/DI/MI/TH Mycobacterium leprae/*/CH/DE/IP/PH Nervos Periféricos/MI Doenças do Sistema Nervoso Periférico/MI/PA Reação em Cadeia da Polimerase Research Support, N.I.H., Extramural Células de Schwann/IM/MI Limits: HUMANO ANIMAL CAMUNDONGOS SUPPORT, NON-U.S. GOV'T Location: BR191.1; 09365/S

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Id: 17690 Author: Rambukkana, Anura Title: Mycobacterium leprae-induced demyelination: a model for early nerve degeneration ..- Source: s.l; s.n; 2004. 8 p. ilus, tab. Abstract: The molecular events that occur at the early phase of many demyelinating neurodegenerative diseases are unknown. A recent demonstration of rapid demyelination and axonal injury induced by Mycobacterium leprae provides a model for elucidating the molecular events of early nerve degeneration which might be common to neurodegenerative diseases of both infectious origin and unknown etiology. The identification of the M. leprae-targeted Schwann cell receptor, dystroglycan, and its associated molecules in myelination, demyelination and axonal functions suggests a role for these molecules in early nerve degeneration. (AU). Descriptors: Axônios/IM/PA/UL Doenças Auto-Imunes Desmielinizantes do Sistema Nervoso Central/ET/*IM/MI/PP Distroglicanas/*IM Complexo de Proteínas Associadas Distrofina/IM Hanseníase/CO/*IM Mycobacterium leprae/*IM Bainha de Mielina/*IM Células de Schwann/*IM/MI/PA Limits: HUMANO ANIMAL CAMUNDONGOS SUPPORT, U.S. GOV'T, NON-P.H.S. SUPPORT, U.S. GOV'T, P.H.S. Location: BR191.1; 09364/S

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Id: 17616 Author: Lopez-Hurtado, Marcela; Flores-Medina, Saul; Díaz-García, Francisco J; Guerra-Infante, Fernando M Title: Partial characterization of phagocytic activity in neutrophils of the nine-banded armadillo Dasypus novemcinctus ..- Source: s.l; s.n; 2005. [7] p. tab. Descriptors: Animais Tatus/*IM Atividade Bactericida do Sangue Quimiotaxia de Leucócito/DE Endocitose Escherichia coli/IM Humanos In Vitro Cinética Hanseníase/IM Monócitos/IM N-Formilmetionina Leucil-Fenilalanina/PD Neutrófilos/DE/*IM Proteínas Opsonizantes/IM Fagocitose/* Location: BR191.1; 09349/S

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Id: 17564 Author: Kramme, Stefanie; Bretzel, Gisela; Panning, Marcus; Kawuma, Joseph; Drosten, Christian Title: Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR ..- Source: s.l; s.n; 2004. 5 p. tab, graf. Abstract: Real-time PCR technology has improved molecular diagnostics of many pathogens, but no such test is available for Mycobacterium leprae. In this report we describe the establishment and the pre-clinical evaluation of such an assay. The test achieved a theoretical analytical sensitivity limit of 194 M. leprae cells per skin biopsy specimen and facilitated quantification of mycobacteria in tissue over a range of 54-54,000,000 cells per sample. In punch skin biopsies from 39 untreated Ugandan patients with newly diagnosed leprosy, the clinical diagnosis could be confirmed in 88.9% of multibacillary and 33.3% of paucibacillary (microscopically negative) patients. Real-time detection thus did not increase the clinical sensitivity of PCR as compared to conventional protocols, in spite of its evidently high analytical sensitivity. On the other hand, as still no culture system exists for M. leprae, the assay appears to be a robust tool for detection of the bacterium in selected clinical situations, as well as for quantitation in experimental settings. (AU). Descriptors: Antígenos de Bactérias/GE Proteínas de Bactérias/GE Biópsia DNA Bacteriano/CH/IP Hanseníase/*DI/MI Dados de Sequência Molecular Mycobacterium leprae/GE/*IP Reação em Cadeia da Polimerase/*MT Sensibilidade e Especificidade Alinhamento de Sequência Análise de Sequência de DNA Pele/MI Uganda Limits: Research Support, Non-U.S. Gov't Humanos Location: BR191.1; 09344/s

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Id: 17561 Author: Geluk, A; van Meijgaarden, K. E; Franken, K. L. M. C; Wieles, B; Arend, S. M; Faber, W. R; Naafs, B; Ottenhoff, T. H. M Title: Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis ..- Source: s.l; s.n; 2004. 5 p. tab, graf. Abstract: Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all tested M. bovis bacille Calmette-Guérin (BCG) strains but present in M. leprae, M. tuberculosis, M. bovis, M. kansasii, M. africanum and M. marinum. In this study, the homologue of CFP-10 in M. leprae (ML0050) is identified and characterized. Interferon-gamma production in response to this homologue by T cells from leprosy patients, TB patients and unexposed controls shows that CFP-10 of M. leprae is a potent antigen that crossreacts with CFP-10 of M. tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases. (AU). Descriptors: Sequência de Aminoácidos Antígenos de Bactérias/IM Proteínas de Bactérias/*IM Reações Cruzadas/IM Interferon Tipo II/IM/SE Hanseníase/*IM Ativação Linfocítica/IM Dados de Sequência Molecular Mycobacterium leprae/*IM Mycobacterium tuberculosis/*IM Homologia de Sequência Linfócitos T/IM/ME Tuberculose/*IM Limits: Research Support, Non-U.S. Gov't Humanos Estudo Comparativo Animais Location: BR191.1; 09340/s

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Id: 17559 Author: Save, M. P; Shetty, V. P; Shetty, K. T; Antia, N. H Title: Alterations in neurofilament protein(s) in human leprous nerves: morphology, immunohistochemistry and Western immunoblot correlative study ..- Source: s.l; s.n; 2004. 16 p. ilus, tab. Abstract: Using a specific antibody (SMI 31), the state of phosphorylation of high and medium molecular weight neurofilaments (NF-H and NF-M) was studied in 22 leprous and four nonleprous human peripheral nerves by means of immunohistochemistry, sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and Western immunoblot (WB). The results thus obtained were compared with morphological changes in the respective nerves studied through light and electron microscopy. Many of the leprous nerves showing minimal pathology revealed lack of or weak staining with SMI 31, denoting dephosphorylation. Remyelinated fibres stained intensely with SMI 31 antibody. The WB analysis of Triton X-100 insoluble cytoskeletal preparation showed absence of regular SMI 31 reactive bands corresponding to 200 and 150 kDa molecular weight (NF-H and NF-M, respectively) in 10 nerves. Three of the 10 nerves revealed presence of NF protein bands in SDS-PAGE but not in WB. Presence of additional protein band (following NF-M) was seen in four nerves. Two nerves revealed NF-H band but not NF-M band and one nerve showed trace positivity. In the remaining five nerves presence of all the three NF bands was seen. Thus, 77.3% (17/22) of human leprous nerves studied showed abnormal phosphorylation of NF protein(s). The ultrastructural study showed abnormal compaction and arraying of NF at the periphery of the axons in the fibres with altered axon to myelin thickness ratio (atrophied fibres) as well as at the Schmidt-Lantermann (S-L) cleft region. Such NF changes were more pronounced in the severely atrophied axons suggesting a direct correlation. The observed well-spaced NF in the remyelinated fibres under ultrastructural study was in keeping with both intense SMI 31 staining and presence of NF triplet bands seen in WBs in four of leprous nerves that showed a large number of regenerating fibres suggesting reversal of changes with regeneration. Findings in the present study suggest that atrophy, that is, the reduction in axonal calibre and paranodal demyelination, seen in leprous nerves may result from dephosphorylation of NF-H and NF-M proteins. (AU). Descriptors: Doença de Alzheimer/PA Esclerose Amiotrófica Lateral/PA Atrofia Axônios/PA Western Blotting Citoesqueleto/ME/PA Doenças Desmielinizantes/PA Eletroforese em Gel de Poliacrilamida Imunohistoquímica Hanseníase/*ME Fibras Nervosas/PA Proteínas de Neurofilamentos/*ME Neurônios/*ME/*PA/UL Inclusão em Parafina Fosforilação Limits: Research Support, Non-U.S. Gov't Humanos Location: BR191.1; 09338/s

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Id: 17318 Author: Black, Gillian F; Weir, Rosemari E; Chaguluka, Steven D; Warndorff, David; Crampin, Amelia C; Mwaungulu, Lorren; Sichali, Lifted; Floyd, Sian; Bliss, Lyn; Jarman, Elizabeth; Docovan, Linda; Andersen, Peter; Britton, Warwick; Hewinson, Glyn; Huygen, Kris; Paulsen, Jean; Sing, Mahavir; Prestidge, Ross; Fine, Paul E. M; Dockrell, Hazel M Title: Gama interferon responses induced by a panel of recombinant and purified mycobacterial antigens in healthy, non-Mycobacterium bovis BCG-vaccinated Malawian young adults ..- Source: s.l; s.n; 2003. 10 p. graf. Abstract: We have previously shown that young adults living in a rural area of northern Malawi showed greater gamma interferon (IFN-gamma) responses to purified protein derivatives (PPD) prepared from environmental mycobacteria than to PPD from Mycobacterium tuberculosis. In order to define the mycobacterial species to which individuals living in a rural African population have been exposed and sensitized, we tested T-cell recognition of recombinant and purified antigens from M. tuberculosis (38 kDa, MPT64, and ESAT-6), M. bovis (MPB70), M. bovis BCG (Ag85), and M. leprae (65 kDa, 35 kDa, and 18 kDa) in >600 non-M. bovis BCG-vaccinated young adults in the Karonga District of northern Malawi. IFN-gamma was measured by enzyme-linked immunosorbent assay (ELISA) in day 6 supernatants of diluted whole-blood cultures. The recombinant M. leprae 35-kDa and 18-kDa and purified native M. bovis BCG Ag85 antigens induced the highest percentages of responders, though both leprosy and bovine tuberculosis are now rare in this population. The M. tuberculosis antigens ESAT-6 and MPT64 and the M. bovis antigen MPB70 induced the lowest percentages of responders. One of the subjects subsequently developed extrapulmonary tuberculosis; this individual had a 15-mm-diameter reaction to the Mantoux test and responded to M. tuberculosis PPD, Ag85, MPT64, and ESAT-6 but not to any of the leprosy antigens. We conclude that in this rural African population, exposure to M. tuberculosis or M. bovis is much less frequent than exposure to environmental mycobacteria such as M. avium, which have antigens homologous to the M. leprae 35-kDa and 18-kDa antigens. M. tuberculosis ESAT-6 showed the strongest association with the size of the Mantoux skin test induration, suggesting that among the three M. tuberculosis antigens tested it provided the best indication of exposure to, or infection with, M. tuberculosis. (AU). Descriptors: Antígenos de Bactérias/*IM Vacina BCG/*IM Interferon Tipo II/*BI Hanseníase/EP Malauí/EP Mycobacterium/GE/*IM Mycobacterium bovis/IM Mycobacterium leprae/IM Mycobacterium tuberculosis/IM Proteínas Recombinantes de Fusão/IM Especificidade de Espécies Linfócitos T/IM Teste Tuberculínico Tuberculose/IM Vacinação Limits: Adolescente Adulto Criança Estudo Comparativo Feminino Humano Masculino População Rural Location: BR191.1; 00224/s

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Id: 13951 Author: Kiszewski, C. A; Becerril, E; Baquera, J; Aguilar, L. D; Hernández-Pando, R Title: Expression of transforming growth factor-beta isoforms and their receptors in lepromatous and tuberculoid leprosy ..- Source: s.l; s.n; 2003. 7 p. ilus, tab, graf. Abstract: Leprosy is an infectious disease with two polar forms, tuberculoid leprosy (TT) and lepromatous leprosy (LL), that are characterized by strong cell-mediated immunity (CMI) and CMI anergy, respectively. Transforming growth factor-beta (TGF-beta) belongs to a family of pleiotropic cytokines (TGF-beta1, TGF-beta2 and TGF-beta3) that participate in the control of cell differentiation and proliferation, as well as tissue repair. This cytokine family is unique because it suppresses CMI. In this study, we compared the expression of the three TGF-beta isoforms and their receptors in skin biopsies from LL and TT patients (LL = 20; TT = 20) using immunohistochemistry and automated morphometry. The percentage of cells immunostained for the three TGF-beta isoforms and cells positive for the three TGF-beta receptors in the inflammatory infiltrate located in the papillary dermis, reticular dermis and periadnexal tissue were significantly higher in LL than that in TT, with macrophages being the most common and strongest immunoreactive cells. Some lymphocytes, fibroblasts, keratinocytes and epithelial cells from sweat glands and hair roots were also positive. In situ reverse-transcription polymerase chain reaction corroborated the capacity of these cells to synthesize TGF-beta1 and TGF-beta receptor 2. This high expression of TGF-beta isoforms and their receptors could contribute to CMI anergy and other clinical characteristic features of leprosy, like skin atrophy. (AU). Descriptors: HANSENIASE VIRCHOWIANA/imunol HANSENIASE VIRCHOWIANA/metab HANSENIASE TUBERCULOIDE/imunol HANSENIASE TUBERCULOIDE/metab MYCOBACTERIUM LEPRAE ISOFORMAS DE PROTEINAS HIBRIDIZACAO IN SITU RNA MENSAGEIRO/quim RNA MENSAGEIRO/genet RECEPTORES DO FATOR DE CRESCIMENTO TRANSFORMADOR BETA/bios RECEPTORES DO FATOR DE CRESCIMENTO TRANSFORMADOR BETA/imunol PELE/citol PELE/imunol REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE REVERSA IMUNOHISTOQUIMICA BIOPSIA FATOR DE CRESCIMENTO TRANSFORMADOR BETA/bios FATOR DE CRESCIMENTO TRANSFORMADOR BETA/genet FATOR DE CRESCIMENTO TRANSFORMADOR BETA/imunol Limits: HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09092/s

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Id: 13904 Author: Contreras Dueñas, Félix; Miguel, S; Roldan, A; Guillén, Javier; Terencio, J; Tarabini, J. Title: Proteinas plasmaticas en la lepra. Source: Revista de Leprologia Fontilles;3(6):467-478, Julio, 1954. tab, graf. Descriptors: HANSENIASE/sangue PROTEINAS/clas Location: BR191.1

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Id: 13722 Author: Maeda, Yumi; Makino, Masahiko; Crick, Dean C; Mahapatra, Sebabrata; Srisungnam, Sopa; Takii, Takemasa; Kashiwabara, Yoshiko; Brennan, Patrick J Title: Novel 33-kilodalton lipoprotein from Mycobacterium leprae ..- Source: s.l; s.n; 2002. 6 p. tab, graf. Abstract: A novel Mycobacterium leprae lipoprotein LpK (accession no. ML0603) was identified from the genomic database. The 1,116-bp open reading frame encodes a 371-amino-acid precursor protein with an N-terminal signal sequence and a consensus motif for lipid conjugation. Expression of the protein, LpK, in Escherichia coli revealed a 33-kDa protein, and metabolic labeling experiments and globomycin treatment proved that the protein was lipidated. Fractionation of M. leprae demonstrated that this lipoprotein was a membrane protein of M. leprae. The purified lipoprotein was found to induce production of interleukin-12 in human peripheral blood monocytes. The studies imply that M. leprae LpK is involved in protective immunity against leprosy and may be a candidate for vaccine design. (AU). Descriptors: SEQUENCIA DE AMINOACIDOS ANTIGENOS DE BACTERIAS/GE/*IM/IP PROTEINAS DE BACTERIAS/GE/*IM/IP CLONAGEM MOLECULAR ESCHERICHIA COLI EXPRESSAO GENICA GENES BACTERIANOS INTERLEUCINA-12/*BI LIPOPROTEINAS/GE/*IM/IP PROTEINAS DE MEMBRANA/GE/*IM/IP DADOS DE SEQUENCIA MOLECULAR PESO MOLECULAR MONOCITOS/IM MYCOBACTERIUM LEPRAE/GE/*IM/IP PRECURSORES DE PROTEINAS/GE/*IM/IP ANALISE DE SEQUENCIA DE DNA Limits: HUMANO SUPPORT, NON-U.S. GOV'T SUPPORT, U.S. GOV'T, P.H.S. Location: BR191.1; 09059/s

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Id: 13720 Author: Brophy, Peter J Title: Subversion of schwann cells and the leper's bell ..- Source: s.l; s.n; May 3, 2002. 2 p. ilus. Descriptors: AXONIOS/PH MORTE CELULAR DIFERENCIACAO CELULAR MEMBRANA CELULAR/ME COCULTURA PROTEINAS CITOESQUELETO/ME DOENCAS DESMIELINIZANTES/*MI DISTROFINA/ME GLICOLIPIDEOS/ME LAMININA/ME HANSENIASE/*MI/PA/*PP GLICOPROTEINAS DE MEMBRANA/ME PROTEINAS DE MEMBRANA/ME MYCOBACTERIUM LEPRAE/GD/ME/*PY BAINHA DE MIELINA/*PH CELULAS DE SCHWANN/CY/*MI/*PH Limits: ANIMAL HUMANO CAMUNDONGOS Location: BR191.1; 09065/s

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Id: 13719 Author: Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P Title: Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..- Source: s.l; s.n; 2003. 5 p. tab. Abstract: To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue PROTEINAS DE BACTERIAS/genet HANSENIASE/diag HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/isol REACAO EM CADEIA DA POLIMERASE/métodos PELE/microbiol - SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO  PRIMERS DO DNA Limits: ESTUDO COMPARATIVO HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09117/s

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Id: 13709 Author: Cardoso, Fernando L. L; Antas, Paulo R. Z; Milagres, Alexandre S; Geluk, Annemieke; Franken, Kees L. M. C; Oliveira, Eliane B; Teixeira, Henrique C; Nogueira, Susie A; Sarno, Euzenir N; Klatser, Paul; Ottenhoff, Tom H. M; Sampaio, Elizabeth P Title: T-cell responses to the Mycobacterium tuberculosis-specific antigen ESAT-6 in Brazilian tuberculosis patients ..- Source: s.l; s.n; 2002. 8 p. tab, graf. Abstract: The Mycobacterium tuberculosis-specific ESAT-6 antigen induces highly potent T-cell responses and production of gamma interferon (IFN-gamma), which play a critical role in protective cell-mediated immunity against tuberculosis (TB). In the present study, IFN-gamma secretion by peripheral blood mononuclear cells (PBMCs) in response to M. tuberculosis ESAT-6 in Brazilian TB patients was investigated in relation to clinical disease types, such as pleurisy and cavitary pulmonary TB. Leprosy patients, patients with pulmonary diseases other than TB, and healthy donors were assayed as control groups. Sixty percent of the TB patients indeed recognized M. tuberculosis ESAT-6, as did 50 per cent of the leprosy patients and 60 per cent of the non-TB controls. Nevertheless, the levels of IFN-gamma in response to the antigen ESAT, but not to antigen 85B (Ag85B) and purified protein derivative (PPD), were significantly lower in controls than in patients with treated TB or pleural or cavitary TB. Moreover, according to Mycobacterium bovis BCG vaccination status, only 59 per cent of the vaccinated TB patients responded to ESAT in vitro, whereas 100 per cent of them responded to PPD. Both CD4 and CD8 T cells were able to release IFN-gamma in response to ESAT. The present data demonstrate the specificity of ESAT-6 of M. tuberculosis and its ability to discriminate TB patients from controls, including leprosy patients. However, to obtain specificity, it is necessary to include quantitative IFN-gamma production in response to the antigen as well, and this might limit the use of ESAT-6-based immunodiagnosis of M. tuberculosis infection in an area of TB endemicity. (AU). Descriptors: ANTIGENOS DE BACTERIAS/DU/GE/*IM LINFOCITOS T CD4-POSITIVOS/*IM LINFOCITOS T CD8-POSITIVOS/*IM INTERFERON TIPO II/BI MYCOBACTERIUM TUBERCULOSIS/*IM PROTEINAS RECOMBINANTES/IM TUBERCULINA/IM TUBERCULOSE PLEURAL/DI/*IM/MI TUBERCULOSE PULMONAR/DI/*IM/MI Limits: FEMININO HUMANO MASCULINO SUPPORT, NON-U.S. GOV'T Location: BR191.1; 09070/s

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